Purification of glutathione S-transferase enzyme from liver tissue of shabout (Barbus grypus Heckel) and investigation of the inhibition effect of some metal ions under in vitro conditions

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dc.contributor.authorBekir Çiftçi
dc.contributor.authorArzu Koçak Mutlu
dc.contributor.authorEbru Akkemik
dc.date.accessioned2025-01-09T11:12:05Z
dc.date.available2025-01-09T11:12:05Z
dc.date.issued2024-12-16
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.descriptionShabout is a fish with high nutritional value and economic potential. This fish is exposed to environmental factors due to the metal toxicity in its habitat and, consequently, its diet. The main purpose of this study was to determine how the detoxification mechanism of shabout is affected by examining the interaction of glutathione s-transferase enzyme with heavy metals. In our study, elemental analysis was first performed with the ICP-OES on water samples taken from three different points to detect metal toxicity in the habitat of the shabout. Then, the GST enzyme from the liver tissue of the shabout was purified for the first time by our team using the glutathione agarose affinity chromatography technique
dc.description.abstractShabout is a fish with high nutritional value and economic potential. This fish is exposed to environmental factors due to the metal toxicity in its habitat and, consequently, its diet. The main purpose of this study was to determine how the detoxification mechanism of shabout is affected by examining the interaction of glutathione s-transferase enzyme with heavy metals. In our study, elemental analysis was first performed with the ICP-OES on water samples taken from three different points to detect metal toxicity in the habitat of the shabout. Then, the GST enzyme from the liver tissue of the shabout was purified for the first time by our team using the glutathione agarose affinity chromatography technique
dc.identifier.citationÇiftçi, B., Koçak Mutlu, A., & Akkemik, E. (2025). Purification of glutathione S-transferase enzyme from liver tissue of shabout (Barbus grypus Heckel) and investigation of the inhibition effect of some metal ions under in vitro conditions. Fish Physiology and Biochemistry, 51(1), 1-9.
dc.identifier.doi10.1007/s10695
dc.identifier.endpage9
dc.identifier.issn0920-1742
dc.identifier.issn1573-5168
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85212277852
dc.identifier.scopusqualityQ3
dc.identifier.startpage1
dc.identifier.urihttps://10.1007/s10695-024-01424-4
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8413
dc.identifier.volume51
dc.identifier.wos001379558700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÇiftçi, Bekir
dc.institutionauthorKoçak Mutlu, Arzu
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofFish Physiology and Biochemistry
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnimals
dc.subjectCyprinidae
dc.subjectGlutathione Transferase
dc.subjectLiver
dc.subjectMetals
dc.subjectHeavy
dc.subjectWater Pollutants
dc.subjectChemical
dc.titlePurification of glutathione S-transferase enzyme from liver tissue of shabout (Barbus grypus Heckel) and investigation of the inhibition effect of some metal ions under in vitro conditions
dc.typejournal-article
oaire.citation.issue1
oaire.citation.volume51

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