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Öğe Mitigating drought stress in fenugreek through synergistic effects of alanine and potassium-enriched biochar(Springer Science and Business Media LLC, 2025-02-03) Wanzheng Ma; Xinyu Fang; Mengru Qiu; Misbah Hareem; Zeki Erden; Çağdaş Can Toprak; Abdullah A. AlarfajDrought stress adversely affects plant growth, development, and yield. It can decrease seed germination, biomass accumulation, root proliferation, chlorophyll contents, and stomatal conductance. To overcome this critical issue, researchers suggest employing environmentally friendly approaches. The exogenous application of alanine (AL) acts as an osmolyte, which helps balance the cellular water under drought stress. It can also improve root architecture, biomass accumulation, and plant fertilizer use efficiency. Applying biochar can improve soil structure, water, and nutrient retention in soil, which are allied factors in enhancing plant growth under drought stress. Furthermore, the enrichment of potassium (K) in biochar also increases its availability to plants, directly regulating the stomatal conductance to alleviate drought stress. That's why the current study aims to explore the combined effect of AL and potassium-enriched biochar (KBC) on fenugreek cultivated under drought stress. Four levels of AL (control, 2mM, 4mM, and 6mM) were applied with 0%, 1%, and 2% KBC in three replicates. Results showed that 2mM AL + 2%KBC showed significant improvement in plant length (5.24%), plant fresh weight (25.36%), plant dry weight (16.23%), chlorophyll a (7.80%), chlorophyll b (15.83%), total chlorophyll (10.65%) over the control under drought stress. A significant increase in shoot N, P, and K concentration compared to control under drought stress also validated the effectiveness of 2mM AL + 2%KBC. In conclusion, 2mM AL + 2%KBC is an effective amendment for alleviating drought stress in fenugreeks. Under drought stress, growers are recommended to apply 2mM AL + 2%KBC to achieve better fenugreek growth.Öğe Distribution of Heat Shock Proteins 27, 60, 70, 90 in Testis and Epididymis of the Domestic Cats (Felis catus) and Dogs (Canis lupus familiaris).(Wiley, 2025-02) Banu Kandil; Alev Gürol BayraktarogluThis study aimed to examine the immunoexpression of HSP27, HSP60, HSP70, and HSP90 in the testis and epididymis of domestic cats (Felis catus) and dogs (Canis lupus familiaris). Testis and epididymis tissues from 6 adult cats and 6 adult dogs were used in this study. Immunohistochemical staining was done to determine the expression of HSPs. In cats and dogs, while HSP60 was detected only in Leydig cells, HSP90 was determined only in spermatogonia. HSP27 was observed only in smooth muscle cells of blood vessels. HSP70 was not detected in spermatocytes, spermatids, Leydig cells, or Sertoli cells, whereas HSP70 was determined in peritubular myoid cells. In addition, unlike cats, HSP70 was observed in spermatogonia of dog testes. HSP27 was determined in basal cells of the epididymal epithelium and smooth muscle cells of the ductal wall in all sections of the epididymis. However, no HSP60 was observed in the epididymis. While HSP70 was not detected in the epididymis of the cats, HSP70 was observed in basal cells of all sections of the epididymis of the dogs. While the epididymal epithelial cells showed HSP90 immunoreactivity in all parts of the epididymis, the smooth muscle cells of the ductal wall exhibited HSP90 immunoreactivity only in the cauda epididymidis. The findings of this study indicate that HSP27, HSP60, HSP70, and HSP90 exhibit different immunoexpression patterns in the testis and epididymis of cats and dogs and that these proteins play important roles in maintaining the reproductive functions of cats and dogs.Öğe Evaluation of the Accuracy and Performance of Two Commercial Pregnancy‐Associated Glycoprotein Tests for Early Pregnancy Detection in Cows(Wiley, 2025-01-28) Tarik Safak; Kenan Çağrı Tümer; Yağmur İpek Alp; Taha Yasin Özen; Oznur Yilmaz‐KocThe aim of this study was to determine and compare the diagnostic accuracies of two commercial pregnancy-associated glycoprotein tests, Alertys OnFarm Pregnancy Test (AOPT) and Alertys Milk Pregnancy Test (AMPT), for early pregnancy diagnosis in dairy cattle. Holstein cows (n = 124) were used in the study. Whole blood samples were collected from the jugular vein 28 days after fixed-time artificial insemination (FTAI). In addition, teats of these cows were swabbed and milk samples were collected into sterile Falcon tubes. AOPT was performed on the farm within 2 h after whole blood collection. Milk samples for AMPT analysis were taken to the laboratory and analysed within 2 h. Transrectal ultrasonography was performed on the 32nd day after FTAI as a reference test. Comparative evaluation was made according to the AOPT and AMPT results, 28 days after FTAI. The sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV) for the AOPT were 92.4%, 80.0%, 87.9%, 89.0% and 85.7% and for AMPT were 97.5%, 82.2%, 91.9%, 90.6% and 94.9% respectively. Cohen's kappa statistic showed a 91.9% agreement (kappa = 0.820, p < 0.001) between the reference test and AMPT, and an 87.9% agreement (kappa = 0.735, p < 0.001) between the reference test and AOPT. AOPT and AMPT offers a reliable, non-invasive (in milk) and practical approach to pregnancy diagnosis in cows. These methods enable early pregnancy detection and can be easily integrated into farm routines, enhancing reproductive management and overall herd productivity.Öğe Different essential oils can inhibit Candida albicans biofilm formation on acrylic resin by suppressing aspartic proteinase: In vitro and in silico approaches(Springer Science and Business Media LLC, 2025-01-28) Nurdan Polat Sağsöz; Leyla Güven; Bahri Gür; Canan Vejselova Sezer; Mustafa Cengiz; Figen Orhan; Özlem BarışIntroduction: Cymbopogon martini, Syzygium aromaticum, and Cupressus sempervirens are used for antimicrobial purposes in the worldwide. Both their extracts and essential oil contents are rich in active ingredients. Objective: The aim of this study was to investigate the inhibitory effect of Cymbopogon martini essential oil (CMEO), Syzygium aromaticum essential oil (SAEO) and Cupressus sempervirens essential oil (CSEO) on Candida albicans biofilm formation on heat-polymerized polymethyl methacrylate (PMMA) samples in vitro and in silico. Materials and methods: Essential oil contents with anticandidal potential were determined by Gas Chromatography-Mass Spectrometry. Following C. albicans adhesion, PMMA samples were treated independently with Corega® and each essential oil. The anticandidal activity of the essential oils was determined by spectrophotometric absorbance measurement at 600 nm, taking into account the cultures of each sample. The cytotoxicity evaluation of essential oils was performed by MTT Colorimetric assay. The software package AutoDockTools (1.5.6) was used for the in silico studies. The effect of essential oil content on the inhibition of Secreted aspartic proteinase (SAP2) was evaluated considering the Ligand@SAP2 complex formation. Results: 2% of CMEO and 5% of SAEO exhibited higher anticandidal activity than Corega® (p < 0.05), whereas Corega® had higher anticandidal activity than 2% and 5% of CSEO (p < 0.05). The cytotoxicity of essential oils on NIH/3T3 cells after 24 h was found to be 2.41 for CSEO, 2.84 for CMEO, and 2.85 µg/mL for SAEO. The results of the in silico study showed that citronellol from CMEO, chavibetol (m-eugenol) from SAEO and β-pinene from CSEO each had the highest effect on the inhibition of SAP2. The highest binding affinity value was found for citronellol at -5.3 kcal/mol. Conclusions: The biofilm formation of C. albicans onto acrylic resin was inhibited by CMEO, SAEO and CSEO at a concentration of 2% through in vitro assay. The most effective inhibition was determined to be due to citronellol in CMEO through in silico analysis.Öğe Chemical profiling and antioxidant potential of propolis from Pervari, Siirt: discovery of tannic acid and key bioactive compounds(Informa UK Limited, 2025-01-24) Erkan Sırma; Mehmet Fidan; Mesut Gök; İbrahim Tegin; Mustafa Abdullah Yılmaz; Erdal YabalakPropolis, a natural product with remarkable therapeutic potential, has gained attention for its antimicrobial, antioxidant, and anti-inflammatory properties. In this study, propolis samples from Sarıyaprak, Kovanağzı, and Çemikari in Pervari, Siirt province, were analysed comprehensively. The evaluation included wax composition, DPPH and FRAP assays, total phenolic and flavonoid content, and pollen analysis. Phytochemical compounds were identified using GC-MS and LC-MS/MS techniques. Among the regions, Çemikari samples exhibited the highest antioxidant activity and phenolic and flavonoid contents, with wax content averaging 60% across all locations. Key compounds identified via RSH-GC/MS included α-pinene, β-pinene, nonadecanal, and nonanal, while GC-MS revealed borneol, sedrenol, and benzyl alcohol. Significant phenolic compounds such as gallic acid, caffeic acid, and tannic acid (reported for the first time in Turkish propolis) were also detected. The high borneol concentration, with its strong anti-inflammatory properties, underscores the unique traits of these samples and their promising antioxidant potential for further research.Öğe Comprehensive Evaluation of Changes in Placentomes in the Second and Third Trimesters of Pregnancy in Cross‐Bred Hamdani Sheep(Wiley, 2025-01) Banu Kandil; Ali Osman Turgut; Davut Koca; Fatma Isbilir; Muhammed Zahid Atli; Barıs Can GuzelBackground: A proper placentation is required for establishment and continuity of pregnancy. In sheep, placentomes are unique structures that enable nutrition and gas exchange between the mother and the foetus. Although placentomes are dynamic formations, there is limited knowledge of changes in placentomes during pregnancy. Objective: This study aimed to identify changes in sheep placentomes in the second and third trimesters of pregnancy using both macroscopic and microscopic methods. Methods: This study investigated 14 healthy cross-breed Hamdani sheep placentomes, comprising seven second and seven third trimesters of pregnancy. The histomorphometric analysis included measurements of capillary number and area in cotyledonary and caruncular regions, while morphometric assessments encompassed placentome dimensions such as number, length, width, and depth. Results: Placentomes were oval and circular in shape in the second and third trimesters. In the second trimester, they were observed as concave structures with thick edges, whereas in the third trimester, they were determined as thin-edged structures with a slight depression in the centre. In the third trimester, foetal and maternal tissues became more intertwined with increased branching of foetal villi and maternal crypts. Placental hematomas and erythrocytes in the cytoplasm of trophoblast cells were more prominent in the third trimester. Statistical analysis revealed no difference in placentome number between the second and third trimesters. However, the dimensions (length, width, and depth) of placentomes were greater in the third trimester compared to the second trimester (p < 0.001). Additionally, while there was no difference in the number of cotyledonary versus caruncular capillaries in the second trimester, cotyledonary capillaries outnumbered caruncular capillaries in the third trimester (p < 0.001). Furthermore, both cotyledonary and caruncular capillary areas increased in the third trimester compared to the second trimester, with the caruncular capillary area being consistently higher than the cotyledonary capillary area in both trimesters (p < 0.05). Conclusion: This study underscores the substantial structural and physiological transformations of placentomes in the second and third trimesters of pregnancy in sheep. These adaptations facilitate efficient flow exchange between the foetus and mother, highlighting the dynamic nature of placental development during late gestation.Öğe Are the Notions of Leader–Member Exchange and Organisational Citizenship Effective in Enhancing Teachers’ Job Performance in Türkiye? A Moderated Mediation Model(MDPI AG, 2025-01-02) Mehmet Sabir ÇevikThis study is correlational and cross-sectional quantitative research that examines the moderating role of working time with the school principal on the effect of leader-member exchange on teachers' job performance through organisational citizenship. Data were collected from 481 teachers in 43 public schools across the western, central, northern, southern, and eastern regions of Türkiye using the convenience sampling method. The Leader-Member Exchange Scale, Organisational Citizenship Scale, and Employee Performance Scale were used as data collection tools. Data were analysed through descriptive analyses, Pearson correlation analysis, and moderator and moderated mediation analyses. The results indicated positive and significant relationships among leader-member exchange, organisational citizenship behaviours, and teachers' job performance. It was found that leader-member exchange indirectly affected teachers' job performance through organisational citizenship, and working time with the school principal served as a moderator in the relationship between leader-member exchange and organisational citizenship. The indirect effect of leader-member exchange on job performance through organisational citizenship was stronger for teachers with shorter working durations with the principal.Öğe Effects of topical neutral protamine Hagedorn insulin on corneal wound healing: an experimental study in rabbits(Informa UK Limited, 2025-01-22) Başak Kurt; İsa Özaydin; Lokman Balyen; Sevil Atalay Vural; Uğur Aydin; Özgür ÖzönerCorneal injuries are common in human and veterinary ophthalmology. There are many studies which have investigated the treatment of corneal epithelial defects. This study aimed to investigate the effects of Neutral Protamine Hagedorn (NPH) insulin as an ointment for wound healing in experimental corneal defects. First, a superficial keratectomy was performed on 12 rabbits using a corneal trephine. The animals were divided into two groups, Group I (treated, n = 6) and Group II (vehicle control, n = 6). Insulin ointment was applied topically once daily in the treated group, and saline ointment was applied in the same manner in the vehicle control group. Corneal defects were observed and photographed, and changes in wound surface were recorded on days 7, 14, 21, 30, and 60. In both groups, a significant reduction in the wound surface area was noticeable on the 30th day after defect creation. Between the 30th and 60th days, while the changes in the wound surface area in Group II remained limited, the decrease continued rapidly in Group I. At the end of the study, the corneal opacity score observed was lower in Group I than in Group II. In conclusion, we determined that topical NPH insulin may accelerate corneal wound healing after superficial lamellar keratectomy. A new alternative treatment may be developed for treating corneal wounds through continuing studies on this subject.Öğe Bullying Among Special Education Students(SAGE Publications, 2025-01-21) Halil Aslan; Özgür Erdur-BakerThe purpose of the study is to examine traditional and cyberbullying among the students with special education needs attending special education schools. Additionally, traditional and cyber victimization among special education students have been examined in terms of gender and grade levels. A sample of the present study consists of 295 students with special education needs (177 gifted, 118 deaf) attending segregated special education schools. The Revised Olweus Bully/Victim Questionnaire, the Revised Cyber Bullying Inventory-II, the Strengths and Difficulties Questionnaire, and a demographic data sheet were employed to collect data. Results of the study indicated that of the total 295 students with special education needs, 28.1% of the students with special education needs were bullies and 39.3% were victims of traditional bullying. Furthermore, 13.5% of the students with special education needs were identified as cyberbullies, and 23.3% of them were found as cybervictims. When gender and grade level were examined in regard to traditional and cyber victimization, significant gender differences were found in 9 and 10 grade levels. Male students with special education needs obtained higher scores for victimization than female students with special education needs. The results of the study were discussed in the light of literature.Öğe Utilizing CT and MRI in Assessing Peritumoral Neovascularization in Renal Cell Carcinoma: A Comprehensive Analysis of Histological Subtypes and Tumor Characteristics by Imaging(Bentham Science Publishers Ltd., 2024-12-30) Murat Tepe; Erce Sevin; Ibrahim Inan; Ahmet Aktan; Muzaffer Ayaz; Heba Ibrahim Ali; Senem SenturkOBJECTIVE: There are variations in prognosis and therapeutic approach for renal cell carcinoma among different histological subtypes. This study aims to determine the relationship between radiologically detected peritumoral neovascularization and the histological subtypes of Renal Cell Carcinoma (RCC) and to assess whether extratumoral neovascularization characteristics detected via imaging can contribute to distinguishing these subtypes alongside tumor size and T-stage. MATERIALS AND METHODS: 104 renal tumors from 104 cases consisting of 31 females (29.8%) and 73 males (70.2%) who underwent abdominal CT or MRI and received a histopathological renal cell carcinoma diagnosis were included. Out of 104 cases, 45 (43.27%) cases had a preoperative CT, 52 (50%) cases had a preoperative MRI, and 7 (6.73%) cases had both preoperative CT and MR images. The cases were categorized according to the histopathologic subtypes. The presence of the radiologically visible peritumoral vascularity and its diameter was noted in order to compare with the histopathological subtypes and other morphologic or histopathological findings, including size, presence of cystic component, T score, and Fuhrman grade of the tumor. RESULTS: 104 unilateral renal tumors (median size 5 cm; range 2-26 cm) were included in this study, of which 71 (68.3%) were clear cell, 20 (9.2%) were papillary and 13 (12.5%) were chromophobe renal cell carcinomas. Although the presence of peritumoral neovascularization was observed to a lesser degree in papillary carcinomas than clear cell and chromophobe carcinomas, there was no statistically significant difference among histological subtypes and between clear cell and non-clear cell carcinomas according to the frequency of peritumoral neovascularization (p = 0.16 and p = 0.084). The presence of peritumoral neovascularization was significantly associated with tumor size for all tumors and within histological subtypes (p < 0.0001). As the diameter of the tumor increased, the presence of peritumoral neovascularization increased. T stage of tumors was significantly associated with both the presence of peritumoral neovascularization and the largest peritumoral vessel diameter (p < 0.01 and p = 0.002). CONCLUSION: No statistically significant association between the histological subtype of tumors and the frequency of peritumoral neovascularization was found in this study. The frequency of peritumoral neovascularization increased with the size and T stage of the tumor. Additionally, the largest peritumoral vessel diameter increased with the T stage of the tumor. There was no statistically significant relationship between peritumoral vascularity and Fuhrman grade.Öğe Is there a significant relationship between the empowering leadership behaviors of school principals and the psychological resilience of teachers? Understanding the moderating effects of gender and length of time spent with the school principal(Springer Science and Business Media LLC, 2025-01-08) Mehmet Sabir Çevik; Emine DoğanThe psychological resilience of teachers has gained significant attention due to its positive effects on schools and students. This cross-sectional quantitative study investigates the moderating roles of gender and the length of time spent with the school principal in the relationship between principals' empowering leadership behaviors and teachers' psychological resilience. Data were collected from 362 teachers working in public primary, secondary, and high schools in Siirt, T & uuml;rkiye. Descriptive statistics, correlation analysis, and moderation analysis using the bootstrapping method were applied to analyze the data. The findings reveal that the empowering leadership behaviors of school principals positively correlate with teachers' psychological resilience, with variations based on gender and time spent with the principal. Specifically, the relationship is stronger for female teachers compared to male teachers and for those who have spent more time with the school principal. These results offer theoretical insights and practical recommendations for enhancing teacher resilience through leadership practices, highlighting the importance of understanding contextual and demographic factors.Öğe Genome-wide discovery of underlying genetic factors associated with fresh and frozen-thawed semen traits in composite ram breeds exhibiting different cryotolerance.(Elsevier BV, 2025-01-08) Bülbül, Bülent; Doğan, Şükrü; Dayanikli, Cemal; Kirbaş, Mesut; Şengül, Ebru; Kal, Yavuz; Yaman, YalçınFewer studies investigate the effects of underlying genetic factors related to semen characteristics, significantly affecting sheep farm profitability. This study aimed to identify single nucleotide polymorphisms (SNP) and genomic regions associated with fresh and frozen-thawed semen traits in rams with low (Hasak) and high (Hasmer) cryotolerance. Semen collected from 11 (5 Hasak with low and 6 Hasmer with high cryotolerance) rams cryopreserved in 0.25 ml straws in the breeding season. Quality characteristics were determined in fresh, equilibrated, and frozen-thawed semen. A Genome-Wide Association Study (GWAS) was conducted to unveil the genetic structure that might be attributed to cryotolerance in low and high cryotoleranced rams. Fresh (regarding total and progressive motility) and equilibrated semen quality were similar in Hasak and Hasmer rams (p > 0.6). However, the freeze-thawing process had a more pronounced negative effect on ram semen traits in Hasak than in Hasmer (p < 0.05). GWAS revealed 27 SNPs correlated with post-thaw semen parameters. Moreover, network analyses revealed pathways related to sperm ion channels and their activities, providing insights into the intricate molecular mechanisms underlying sperm physiology and emphasizing their role in potentially impacting sperm cryotolerance. The functional significance of detected SNPs and the associated pathways require further exploration.Öğe High-Sensitive Cardiac Troponin I and Th1/Th2 Polarization in Queens With Pyometra.(Veterinary Medicine and Science, 2025-01) Safak, Tarik; Saat, Nevzat; Yilmaz-Koc, Oznur; Turanli, Mert; Ayalp-Erkan, Aslıhan; Risvanli, AliTo determine T helper (Th)1 and Th2 cytokine polarization, as well as high-sensitive cardiac troponin I (hs-cTnI) levels, in cats with pyometra.Öğe The Effect of Cholesterol-Loaded Cyclodextrin and Resveratrol Compounds on Post-Thawing Quality of Ram Semen.(Wiley, 2025-01) Ahmet, Eser; Ramazan, Arıcı; Selin, Yağcıoğlu; İzem, Sandal Asiye; Nur, Ersoy; Kamber, Demir; Mithat, Evecen; Kemal, AkRam sperm are more vulnerable to freezing than those of most other farm animals. During sperm freezing, the cell membrane loses some of its cholesterol, which regulates signalling mechanisms and prevents premature capacitation. Resveratrol (RES) increases the fluidity of the cell membrane, which becomes peroxidized during freezing and reduces free radicals. In this study, the effectiveness of RES, cholesterol-loaded cyclodextrin (CLC) and their combinations in ram sperm cryopreservation were investigated. The collected semen was divided into two equal volumes: One was diluted with tris-citric acid-glucose medium (TCG) containing CLC, whereas the other was diluted with a CLC-free TCG solution. After examining motility, both groups were further divided into two equal volumes, forming the following working groups: control (no RES, no CLC); RES (20 µg/mL); CLC (2 mg CLC/120 × 10 sperm); and RES + CLC (RES 20 µg/mL + 2 mg CLC/120 × 10 sperm). These groups were diluted with media containing their respective additives. Post-thawing, the samples were analysed for motility, acrosome and membrane integrity, membrane functionality, mitochondrial activity, capacitation status, oxidative stress and DNA integrity. CLC preserved sperm total motility, acrosome and plasma membrane integrity and decreased the rate of early capacitation (p < 0.05). RES had no significant effect on sperm quality before freezing and post-thawing (p > 0.05). However, RES + CLC increased mitochondrial activity post-thawing (p < 0.05). In conclusion, CLC minimized sperm membrane damage caused by cryopreservation in ram sperm. RES alone was ineffective, and the combination of RES and CLC did not yield a positive synergistic effect on ram spermatological parameters.Öğe The Relationship of Th/Th Cytokine Polarization at Parturition in Cows and SOCS3 Level With Some Postpartum Diseases.(2025-01) Yilmaz Koc, Oznur; Risvanli, AliTh/Th polarisation and suppressor of cytokine signalling-3 (SOCS3) are important indicators of the humoral and cellular immune system activity in cows. The aim of this study was to determine the correlation of postpartum diseases with the levels of Th/Th polarisation and SOCS3 at the time of parturition. The study examined 180 cows (90 with normal parturition [NP] and 90 with dystocia [D]). Blood samples were taken from the cows once at the time of calving. Two subgroups were created among cows with NP: those without the postpartum disease (NP [-], n = 45) and those with postpartum disease (NP [+], n = 45). Likewise, two subgroups were created among D cows: those without postpartum disease (D [-], n = 45) and those with postpartum disease (D [+], n = 45). Cytokine analyses were performed using species-specific commercial ELISA kits. In the NP (-) group, it was found that Th/Th cytokine polarisation was in the Th direction due to the increase in the concentration of IFN-γ, TNF-α and IL-2 in four subgroups grouping with different types of parturition and diseases. It was concluded that it would be appropriate to strengthen cellular immunity. In cases of postpartum diseases, Th/Th polarisation shifted towards Th due to the increase in IL-4 and IL-5 concentrations in cows that performed NP and developed mastitis in the postpartum period. These results suggest that it would be beneficial to support the Th aspect (i.e. humoral immunity) in cows that have undergone NP and develop mastitis in the postpartum period.Öğe Identification of significant SNPs for yield-related salt tolerant traits in rice through genome-wide association analysis.(Cell Mol Biol, 2025-01-12) Era, Farzana Mustafa; Raihan, Mohammad Sharif; Jahan, Nusrat; Pandey, Saurabh; Alalawy, Adel I; Al-Duais, Mohammed Ali; Alharbi, Basmah M; Alqurashi, Mohammed; Erden, Zeki; Toprak, Çağdaş Can; Islam, A K M AminulRice salt tolerance is highly anticipated to meet global demand in response to decreasing farmland and soil salinization. Therefore, dissecting the genetic loci controlling salt tolerance in rice for improving productivity is of utmost importance. Here, we evaluated six salt-tolerance-related traits of a biparental mapping population comprising 280 F2 rice individuals (Oryza sativa L.) at the seedling and reproductive stages. We performed a genome-wide association study (GWAS) to identify marker-trait associations under artificially induced salt stress using the 1K RICA chip (Agriplex Genomics, Cedar Avenue, Suite 250, Cleveland, 011444106, USA). We have identified 8 single nucleotide polymorphisms (SNPs) representing eight genomic regions on chromosomes 5, 8, 9, and 10. These were significantly associated with the six salt-tolerance-related traits, no. of tillers per plant (TPP), effective tillers per plant (ETP), spikelet fertility percentage (SFP), field grain number (FGN), grain length breadth ratio (LBR) and thousand-grain weight (TGW). FGN has two significant SNPs (SNP0758 and SNP0759) on Chromosome 9, whereas SFP on chromosomes 8 and 12 (SNP1127 and SNP0966, respectively). Similarly, for TPP (SNP0796), a significant SNP was detected on chromosome 10, and for ETP (SNP0414) on chromosome 5. Two significant SNPs were found in chromosome 12 for LBR (SNP0920) and TGW (SNP0976). Based on all loci, we screened 3 possible candidate genes in chromosomes 8, 9, and 12 between the genomic region of SNP0920 and SNP1127 under salt stress. Interestingly, these genes were involved in protein coding, none of which was previously reported as being involved in plant salt tolerance. Further, the genetic relationship between the mapping population and population structure was classified by STRUCTURE v 2.3. Genotypes with ≥ 80% of shared ancestry were explained into two major clusters (I and II), and < 80% of shared ancestry were categorized as admixtures. An unrooted alpha was developed by TASSEL 5.0, dividing the genotypes into three major groups where 97 individuals were in Cluster 1, cluster 2 consisted of 93 individuals, and the remaining Cluster 3 included 90 individuals. A kinship matrix developed from 860 SNPs indicated group formation and more substantial relatedness among the genotypes with a red zone. Our findings provide valuable information for enhancing the understanding of complicated salt tolerance mechanisms in rice seedlings and the identified candidates potentially used for breeding salt-tolerant genotypes.Öğe Investigation of the presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis in infectious keratoconjunctivitis cases in sheep and goats in Siirt province and evaluation of clinical findings(Polish Academy of Sciences Chancellery, 2024-06-12) A. Gulaydin; O. Gulaydin; M.B. Akgul; N. Sindak; O. YildirimInfectious keratoconjunctivitis is an infectious disease that negatively affects animal welfare causing systemic or local clinical signs in small ruminants and causes significant economic losses in herds. It is important to determine the etiologic agent causing the infection in the development of the protection and control strategies against the disease. The aim of this study was to determine the presence of infectious keratoconjunctivitis cases in small ruminants raised in Siirt province in Türkiye. Infectious keratoconjunctivitis was graded according to the symptoms determined by clinical examination. The presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis was investigated by PCR in swab samples obtained from the animals with keratoconjunctivitis. Infectious keratoconjunctivitis was detected in 263 (19.86%) of 1324 animals examined in the study. Of the animals with infectious keratoconjunctivitis, 163 (61.97%) were sheep and 100 (38.02%) were goats. The detection rate of infectious keratoconjunctivitis was higher in sheep than goats. In 56 (21.29%), 109 (41.44%), 67 (25.47%), and 31 (11.78%) of the cases, findings of stage 1, 2, 3, and 4 infectious keratoconjunctivitis were detected, respectively. Of the eye swab samples taken from 263 animals with infectious keratoconjunctivitis, 5 (1.90%) were positive for Mycoplasma spp. and 6 (2.28%) were positive for M. ovis. It was determined that the distribution of the bacterial agents varied according to the stage of infectious keratoconjunctivitis. No statistically significant correlation was found in the distribution of bacterial agents among identified samples according to species, sex, age, and infectious keratoconjunctivitis stage of the animals. It was thought that the data obtained in the study would contribute to the studies for protection and control by determining the incidence and aetiology of infectious keratoconjunctivitis cases observed in small ruminants. © 2024 The Authors. This is an open access article under the CC BY-NC-ND 4.0 license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en), which allows re-users to copy and distribute the material in any medium or format in unadapted form and for noncommercial purposes, and only so long as attribution is given to the creator.Öğe Investigation of growth traits in Turkish Merino lambs using multi-locus GWAS approaches: Middle Anatolian Merino(Springer Science and Business Media LLC, 2024-12-19) Yalçın Yaman; Şükrü DOĞAN; Mesut KIRBAŞ; A. Taner ÖNALDI; Yavuz KALThis study explored the genetic basis of growth traits in Middle Anatolian Merino lambs using multi-locus genome-wide association study (GWAS) analyses. Descriptive statistics indicated moderate heritability (h² = 0.363) for birth weight (BW) and (h² = 0.309) for weaning weight (WW), both statistically significant (p < 0.001). Strong genetic correlations were observed between WW and BW (rG = 0.922) and WW and Kleiber ratio (KR, rG = 0.896), implying that simultaneous improvements may be possible through targeted selection. Five multi-locus methods (mrMLM, FASTmrMLM, pLARmEB, FASTmrEMMA, and ISIS EM-BLASSO) were used to identify the polygenic basis of the traits. For BW and WW, 20 and 18 significant SNPs (LOD ≥ 5) were detected, respectively, with some SNPs co-detected by multiple methods. In contrast, only 10 significant SNPs were identified for KR, all exclusively by the ISIS EM-BLASSO approach. Pathway analyses within ± 100 Kb of associated SNPs revealed genes and pathways influencing these traits, which could be leveraged in future breeding programs for enhanced growth performance. The identified SNPs, particularly those associated with BW and WW traits, could facilitate genomic selection if validated in larger and more diverse populations.Öğe Investigation of growth traits in Turkish Merino lambs using multi-locus GWAS approaches: Middle Anatolian Merino(Springer Science and Business Media LLC, 2024-12-19) Yalçın Yaman; Şükrü DOĞAN; Mesut KIRBAŞ; A. Taner ÖNALDI; Yavuz KALThis study explored the genetic basis of growth traits in Middle Anatolian Merino lambs using multi-locus genome-wide association study (GWAS) analyses. Descriptive statistics indicated moderate heritability (h² = 0.363) for birth weight (BW) and (h² = 0.309) for weaning weight (WW), both statistically significant (p < 0.001). Strong genetic correlations were observed between WW and BW (rG = 0.922) and WW and Kleiber ratio (KR, rG = 0.896), implying that simultaneous improvements may be possible through targeted selection. Five multi-locus methods (mrMLM, FASTmrMLM, pLARmEB, FASTmrEMMA, and ISIS EM-BLASSO) were used to identify the polygenic basis of the traits. For BW and WW, 20 and 18 significant SNPs (LOD ≥ 5) were detected, respectively, with some SNPs co-detected by multiple methods. In contrast, only 10 significant SNPs were identified for KR, all exclusively by the ISIS EM-BLASSO approach. Pathway analyses within ± 100 Kb of associated SNPs revealed genes and pathways influencing these traits, which could be leveraged in future breeding programs for enhanced growth performance. The identified SNPs, particularly those associated with BW and WW traits, could facilitate genomic selection if validated in larger and more diverse populations.Öğe Modeling and analysis of dengue transmission in fuzzy-fractional framework: a hybrid residual power series approach(Springer Science and Business Media LLC, 2024-12-28) Mubashir Qayyum; Qursam Fatima; Ali Akgül; Murad Khan HassaniThe current manuscript presents a mathematical model of dengue fever transmission with an asymptomatic compartment to capture infection dynamics in the presence of uncertainty. The model is fuzzified using triangular fuzzy numbers (TFNs) approach. The obtained fuzzy-fractional dengue model is then solved and analyzed through fuzzy extension of modified residual power series algorithm, which utilizes residual power series along with Laplace transform. Numerical analysis has also been performed in this study and obtained results are shown as solutions and residual errors for each compartment to ensure the validity. Graphical analysis depict the model’s behavior under varying parameters, illustrating contrasting trends for different values of and examining the impacts of transmission and recovery rates on dengue model in uncertain environment. The current findings highlighted the effectiveness of proposed uncertainty in epidemic system dynamics, offering new insights with potential applications in other areas of engineering, science and medicine.
