Purification of glutathione S-transferase enzyme from liver tissue of shabout (Barbus grypus Heckel) and investigation of the inhibition effect of some metal ions under in vitro conditions
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Tarih
2024-12-16
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Science and Business Media LLC
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Shabout is a fish with high nutritional value and economic potential. This fish is exposed to environmental factors due to the metal toxicity in its habitat and, consequently, its diet. The main purpose of this study was to determine how the detoxification mechanism of shabout is affected by examining the interaction of glutathione s-transferase enzyme with heavy metals. In our study, elemental analysis was first performed with the ICP-OES on water samples taken from three different points to detect metal toxicity in the habitat of the shabout. Then, the GST enzyme from the liver tissue of the shabout was purified for the first time by our team using the glutathione agarose affinity chromatography technique
Açıklama
Shabout is a fish with high nutritional value and economic potential. This fish is exposed to environmental factors due to the metal toxicity in its habitat and, consequently, its diet. The main purpose of this study was to determine how the detoxification mechanism of shabout is affected by examining the interaction of glutathione s-transferase enzyme with heavy metals. In our study, elemental analysis was first performed with the ICP-OES on water samples taken from three different points to detect metal toxicity in the habitat of the shabout. Then, the GST enzyme from the liver tissue of the shabout was purified for the first time by our team using the glutathione agarose affinity chromatography technique
Anahtar Kelimeler
Animals, Cyprinidae, Glutathione Transferase, Liver, Metals, Heavy, Water Pollutants, Chemical
Kaynak
Fish Physiology and Biochemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
51
Sayı
1
Künye
Çiftçi, B., Koçak Mutlu, A., & Akkemik, E. (2025). Purification of glutathione S-transferase enzyme from liver tissue of shabout (Barbus grypus Heckel) and investigation of the inhibition effect of some metal ions under in vitro conditions. Fish Physiology and Biochemistry, 51(1), 1-9.
