A Novel Quantitative Volumetric Spreading Index Definition and Assessment of Astrocyte Spreading In Vitro

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dc.authorid0000-0003-1824-5260en_US
dc.contributor.authorTİRYAKİ, VOLKAN MÜJDAT
dc.contributor.authorAYRES, VIRGINIA M
dc.contributor.authorAHMED, IJAZ
dc.contributor.authorSHREIBER, DAVID I
dc.date.accessioned2019-11-29T05:34:20Z
dc.date.available2019-11-29T05:34:20Z
dc.date.issued2017en_US
dc.departmentMühendislik Fakültesien_US
dc.description.abstractA novel quantitative volumetric spreading index (VSI) is defined that depends on the total distance between object voxels and the contact surface plane in three-dimensional (3D) space. The VSI, which ranges from 0 to 1, is rotationally invariant around the zaxis. VSI can be used to quantify the degree of individual cell spreading, which is important for analysis of cell interactions with their environment. The VSIs of astrocytes cultured on a nanofibrillar surface and three different comparative planar surfaces have been calculated from confocal laser scanning microscope z-series images, and the effects of both culture surface and immunoreactivity on the degree of cell spreading were investigated. VSI calculations indicated a statistical correlation between increased reactivity, based on immunolabeling for glial fibrillary acidic protein, and decreased cell spreading. Further results provided a quantitative measure for the increased spreading of quiescent-like and reactive-like astrocytes on planar substrates functionalized with poly-L-lysine.en_US
dc.identifier.citationTiryaki, VM, Ayres, VM, Ahmed, I, Shreiber, DI. A novel quantitative volumetric spreading index definition and assessment of astrocyte spreading in vitro. Cytometry: Part A, 2017, 91A(8): 794-799 .en_US
dc.identifier.doi10.1002/cyto.a.23183
dc.identifier.endpage799en_US
dc.identifier.pmid28746797
dc.identifier.scopus2-s2.0-85026360573
dc.identifier.scopusqualityQ1
dc.identifier.startpage794en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12604/2103
dc.identifier.volume91Aen_US
dc.identifier.wosWOS:000408333700008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTİRYAKİ, VOLKAN MÜJDAT
dc.language.isoenen_US
dc.publisherInternational Society for Advancement of Cytometryen_US
dc.relation.ispartofCytometry Part A
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKG_20241224
dc.subjectCell morphologyen_US
dc.subjectQuantitative cell spreadingen_US
dc.subject3D shape descriptoren_US
dc.subjectImmunoreactivityen_US
dc.subjectConfocal laser scanning microscopeen_US
dc.titleA Novel Quantitative Volumetric Spreading Index Definition and Assessment of Astrocyte Spreading In Vitroen_US
dc.typeArticleen_US

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