A Novel Quantitative Volumetric Spreading Index Definition and Assessment of Astrocyte Spreading In Vitro
Yükleniyor...
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
International Society for Advancement of Cytometry
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
A novel quantitative volumetric spreading index (VSI) is defined that depends on the
total distance between object voxels and the contact surface plane in three-dimensional
(3D) space. The VSI, which ranges from 0 to 1, is rotationally invariant around the zaxis. VSI can be used to quantify the degree of individual cell spreading, which is
important for analysis of cell interactions with their environment. The VSIs of astrocytes cultured on a nanofibrillar surface and three different comparative planar surfaces
have been calculated from confocal laser scanning microscope z-series images, and the
effects of both culture surface and immunoreactivity on the degree of cell spreading
were investigated. VSI calculations indicated a statistical correlation between increased
reactivity, based on immunolabeling for glial fibrillary acidic protein, and decreased
cell spreading. Further results provided a quantitative measure for the increased spreading of quiescent-like and reactive-like astrocytes on planar substrates functionalized
with poly-L-lysine.
Açıklama
Anahtar Kelimeler
Cell morphology, Quantitative cell spreading, 3D shape descriptor, Immunoreactivity, Confocal laser scanning microscope
Kaynak
Cytometry Part A
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
91A
Sayı
Künye
Tiryaki, VM, Ayres, VM, Ahmed, I, Shreiber, DI. A novel quantitative volumetric spreading index definition and assessment of astrocyte spreading in vitro. Cytometry: Part A, 2017, 91A(8): 794-799 .
