Covalent immobilization of aspergillus niger amyloglucosidase (ANAG) with ethylenediamine-functionalized and glutaraldehyde-activated active carbon (EFGAAC) obtained from sesame seed shell

Basit Öğe Kaydı
dc.contributor.authorSharif, Yousif Mohammed
dc.contributor.authorŞahin, Ömer
dc.contributor.authorAslan, Yakup
dc.date.accessioned2020-01-20T07:43:03Z
dc.date.available2020-01-20T07:43:03Z
dc.date.issued2020en_US
dc.departmentMühendislik Fakültesien_US
dc.description.abstractThis study was aimed the covalently immobilization of Aspergillus niger amyloglucosidase (ANAG) onto activated carbon (AC) obtained from sesame seed shell. AC was firstly functionalized with ethylenediamine, and after then activated with glutaraldehyde. 99.80% immobilization yield and 99.83% activity yield were obtained as the result of optimization of immobilization conditions (pH and molarity of immobilization buffer, AC amount, and reaction time). The optimum pH (5.5) and the optimum temperature range (55–60 C) for ANAG were not affected by immobilization. After immobilization, Vmax value decreased from 1464.1 lmol D-glucose/L.min to 1342.3 lmol D-glucose/L.min, while Km value decreased from 116.3 g maltodextrin/L to 109.9 g maltodextrin/L. The immobilized enzyme retained 99.30% and 98.30% of its initial activity, respectively after twenty repeated uses and after twenty days of storage in 5 mL sodium phosphate buffer (0.1 M, pH 5.5) at +4 C in a refrigerator. Finally, glucose syrup was produced from maltodextrin solution having 1% (w/v) concentration by using the immobilized ANAG. Maltodextrin was completely converted to glucose after four hours. Consequently, it can be said that the immobilized ANAG obtained in this study can be used in the industrial production of glucose syrup.en_US
dc.description.sponsorshipSiirt Üniversitesi BAP Koordinatörlüğü. Proje Kodu: 2017-SIÜFEB-28en_US
dc.identifier.citationAslan, Y., Sharif, Y. M., & Şahin, Ö. (2020). Covalent immobilization of Aspergillus niger amyloglucosidase (ANAG) with ethylenediamine-functionalized and glutaraldehyde-activated active carbon (EFGAAC) obtained from sesame seed shell. International journal of biological macromolecules, 142, 222-231.en_US
dc.identifier.doi10.1016/j.ijbiomac.2019.09.226
dc.identifier.endpage231en_US
dc.identifier.issue2020en_US
dc.identifier.pmid31726168
dc.identifier.scopus2-s2.0-85075497327
dc.identifier.scopusqualityQ1
dc.identifier.startpage222en_US
dc.identifier.urihttps://reader.elsevier.com/reader/sd/pii/S0141813019322895?token=65DCA89CCFD0237A0C91BB85AC51E4BB6F1367CAE4F4A0F4C93395EC135A78A2FFFA0A9B696AF1F5A602A4FA9B03AB84
dc.identifier.urihttps://hdl.handle.net/20.500.12604/2613
dc.identifier.volume142en_US
dc.identifier.wosWOS:000510955500020
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorŞahin, Ömer
dc.institutionauthorAslan, Yakup
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.snmzKG_20241224
dc.subjectActivated carbonen_US
dc.subjectAmyloglucosidaseen_US
dc.subjectCovalent immobilizationen_US
dc.titleCovalent immobilization of aspergillus niger amyloglucosidase (ANAG) with ethylenediamine-functionalized and glutaraldehyde-activated active carbon (EFGAAC) obtained from sesame seed shellen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Aslan et al.2020-Covalent immobilization ofAspergillus nigeramyloglucosidase (ANAG)with ethylenediamine-functionalized and glutaraldehyde-activatedactive carbon (EFGAAC) obtained from sesame seed shell.pdf
Boyut:
998.22 KB
Biçim:
Adobe Portable Document Format
Açıklama:
makale
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

2) Makale
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WOS İndeksli Yayınlar Koleksiyonu