Covalent immobilization of aspergillus niger amyloglucosidase (ANAG) with ethylenediamine-functionalized and glutaraldehyde-activated active carbon (EFGAAC) obtained from sesame seed shell
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/restrictedAccess
Özet
This study was aimed the covalently immobilization of Aspergillus niger amyloglucosidase (ANAG) onto
activated carbon (AC) obtained from sesame seed shell. AC was firstly functionalized with ethylenediamine,
and after then activated with glutaraldehyde. 99.80% immobilization yield and 99.83% activity
yield were obtained as the result of optimization of immobilization conditions (pH and molarity of immobilization
buffer, AC amount, and reaction time). The optimum pH (5.5) and the optimum temperature
range (55–60 C) for ANAG were not affected by immobilization. After immobilization, Vmax value
decreased from 1464.1 lmol D-glucose/L.min to 1342.3 lmol D-glucose/L.min, while Km value decreased
from 116.3 g maltodextrin/L to 109.9 g maltodextrin/L. The immobilized enzyme retained 99.30% and
98.30% of its initial activity, respectively after twenty repeated uses and after twenty days of storage
in 5 mL sodium phosphate buffer (0.1 M, pH 5.5) at +4 C in a refrigerator. Finally, glucose syrup was produced
from maltodextrin solution having 1% (w/v) concentration by using the immobilized ANAG.
Maltodextrin was completely converted to glucose after four hours. Consequently, it can be said that
the immobilized ANAG obtained in this study can be used in the industrial production of glucose syrup.
Açıklama
Anahtar Kelimeler
Activated carbon, Amyloglucosidase, Covalent immobilization
Kaynak
International Journal of Biological Macromolecules
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
142
Sayı
2020
Künye
Aslan, Y., Sharif, Y. M., & Şahin, Ö. (2020). Covalent immobilization of Aspergillus niger amyloglucosidase (ANAG) with ethylenediamine-functionalized and glutaraldehyde-activated active carbon (EFGAAC) obtained from sesame seed shell. International journal of biological macromolecules, 142, 222-231.