Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats

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dc.authoridbengu, aydin sukru/0000-0002-7635-4855
dc.authoridBayramoglu AKKOYUN, Mahire/0000-0001-5150-5402
dc.contributor.authorTemel, Yusuf
dc.contributor.authorBengu, Aydin Sukru
dc.contributor.authorAkkoyun, Hurrem Turan
dc.contributor.authorAkkoyun, Mahire
dc.contributor.authorCiftci, Mehmet
dc.date.accessioned2024-12-24T19:24:11Z
dc.date.available2024-12-24T19:24:11Z
dc.date.issued2017
dc.departmentSiirt Üniversitesi
dc.description.abstractIn this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2,5-ADP-Sepharose 4B affinity gel. The effects of Ast and Al3+ ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al3+ inhibited the enzyme activity noncompetitively (IC50 values; 0.679mM, K-i values 1.32mM). For in vivo studies, the rats were divided into the groups: control (Cont.), Al, Ast, and Al+Ast. The last three groups were compared with the control group. In Al group, a significant degree of inhibition was observed in the activity of G6PD and 6PGD enzymes when compared with the control group (P<0.05), whereas there was an increase in the activities of G6PD and 6PGD enzymes in Ast and Al+Ast groups (P<0.05).
dc.identifier.doi10.1002/jbt.21954
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue10
dc.identifier.pmid28665548
dc.identifier.scopus2-s2.0-85021718152
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/jbt.21954
dc.identifier.urihttps://hdl.handle.net/20.500.12604/5883
dc.identifier.volume31
dc.identifier.wosWOS:000412328200013
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Biochemical and Molecular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectaluminum
dc.subjectastaxanthin
dc.subjectenzyme purification
dc.subjectglucose-6-phosphate dehydrogenase (G6PD)
dc.subject6-phosphogluconate dehydrogenase (6PGD)
dc.subjectinhibition
dc.subjectrat erythrocyte
dc.titleEffect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats
dc.typeArticle

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