Expression of hCA IX isoenzyme by using sumo fusion partner and examining the effects of antitumor drugs

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dc.contributor.authorYerlikaya, Emrah
dc.contributor.authorErdogan, Orhan
dc.contributor.authorDemirdag, Ramazan
dc.contributor.authorSenturk, Murat
dc.contributor.authorKufrevioglu, Omer Irfan
dc.date.accessioned2019-11-18T10:39:37Z
dc.date.available2019-11-18T10:39:37Z
dc.date.issued2015
dc.departmentBelirleneceken_US
dc.description.abstractObjective: In this study, investigating the effects of inhibition of the enzyme activity of some antitumor drugs and the Cancer-Related Human Carbonic Anhydrase IX (hCA IX) isoenzyme expressing as a SUMO fusion protein in an Escherichia coli expression system were aimed. Methods: hCA IX isoenzyme was expressed using SUMO fusion technology. The fusion protein was expressed in a totally soluble form and the expression was verified by SDS-PAGE analysis. Affinity chromatography was used in the purification processes. The effects of certain antitumor drugs on enzyme activity were investigated in vitro conditions by using esterase activity. IC50 values of drugs showing the inhibitory effect were calculated. Inhibition types and Ki values for antitumor drugs, which inhibit the enzyme, were determined by separately plotting Lineweaver- Burk plots. Results: The molecular weight of the fusion protein was approximately 85kDa. The optimal induction concentration of IPTG and the growth temperature were found to be 1.0mM and 30oC. The fusion protein was purified at approximately 3.07-fold with a yield of 92.58%, and a specific activity of 43707EU/mg proteins by nickel nitrilo-triacetic acid resin chromatography.en_US
dc.identifier.scopus2-s2.0-84939512690
dc.identifier.scopusqualityQ3
dc.identifier.trdizinid206206
dc.identifier.urihttps://hdl.handle.net/20.500.12604/1616
dc.identifier.wosWOS:000362430700009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.relation.publicationcategoryUlusal Hakemli Dergi Makalesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKG_20241224
dc.subjectCloning, expression, cancer, carbonic anhydraseen_US
dc.titleExpression of hCA IX isoenzyme by using sumo fusion partner and examining the effects of antitumor drugsen_US
dc.typeArticleen_US

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