Bioactive Apigenin‐7‐<i>O</i>‐β‐Glucoside and Rosmarinic Acid Molecules From Two <i>Nepeta</i> Species: Bioactivity‐Guided Isolation, In Vitro Evaluations, Pharmacokinetic and In Silico Approaches as Metabolic Enzyme Inhibition Agents

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dc.contributor.authorMesut Gok
dc.contributor.authorYunus Basar
dc.contributor.authorSemiha Yenigun
dc.contributor.authorYasar Ipek
dc.contributor.authorLutfi Behcet
dc.contributor.authorTevfik Ozen
dc.contributor.authorIbrahim Demirtas
dc.date.accessioned2025-04-29T06:44:02Z
dc.date.available2025-04-29T06:44:02Z
dc.date.issued2025-04-27
dc.departmentFakülteler, Veteriner Fakültesi, Gıda Hijyeni ve Teknolojisi Bölümü
dc.description.abstractIntroduction: In this study, apigenin-7-O-β-glucoside (AGL) was isolated from Nepeta nuda; also, rosmarinic acid (RA) was isolated from N. aristata and N. nuda. Objectives: The aim of this study is to investigate the enzyme inhibitory, DNA protective, and antibacterial effects of AGL and RA isolated from two Nepeta species. Material and methods: 1D and 2D NMR spectra and an MS chromatogram were recorded to identify AGL and RA. The antibacterial and DNA protection activities, enzyme inhibition, and kinetics investigated of AGL and RA. Molecular interactions, molecular dynamics (MD) simulations, molecular mechanics Poisson-Boltzmann surface area (MM-PBSA) calculations, density functional theory (DFT), molecular electrostatic potential (MEP) analyses, and prediction of activity spectra for substances (PASS) predictions for RA and AGL were investigated for the first time to evaluate the activity results. Results: In this context, the inhibitory properties of AGL were higher in urease, α-amylase, and tyrosinase, whereas RA has a higher inhibitory activity on lipase, CA, and urease. In addition, AGL and RA showed effective antimicrobial activity against Staphylococcus aureus, while it also was effective DNA and deoxyribose protective activities. The stability of the complex formed by lipase, CA, and urease with RA and by tyrosinase and α-amylase with AGL was determined by MD simulations, and the energy results of RA were evaluated by MM-PBSA analysis. The DFT, MEP analysis, and PASS prediction showed that AGL and RA have a soft structure and can easily exchange electrons. Conclusion: According to the results obtained from the current study, AGL and RA were explored as a drug model.
dc.identifier.doi10.1002/pca.3536
dc.identifier.issn0958-0344
dc.identifier.issn1099-1565
dc.identifier.pmid40288811
dc.identifier.urihttps://doi.org/10.1002/pca.3536
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8637
dc.indekslendigikaynakPubMed
dc.institutionauthorGök, Mesut
dc.institutionauthorid0000-0001-9217-8022
dc.publisherWiley
dc.relation.ispartofPhytochemical Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectNepeta aristata
dc.subjectNepeta nuda
dc.subjectapigenin‐7‐O‐β‐glucoside
dc.subjectin silico study
dc.subjectin vitro bioactivity
dc.subjectrosmarinic acid.
dc.titleBioactive Apigenin‐7‐<i>O</i>‐β‐Glucoside and Rosmarinic Acid Molecules From Two <i>Nepeta</i> Species: Bioactivity‐Guided Isolation, In Vitro Evaluations, Pharmacokinetic and In Silico Approaches as Metabolic Enzyme Inhibition Agents
dc.typejournal-article

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