The Effect of Cholesterol-Loaded Cyclodextrin and Resveratrol Compounds on Post-Thawing Quality of Ram Semen.

Basit Öğe Kaydı
dc.contributor.authorAhmet, Eser
dc.contributor.authorRamazan, Arıcı
dc.contributor.authorSelin, Yağcıoğlu
dc.contributor.authorİzem, Sandal Asiye
dc.contributor.authorNur, Ersoy
dc.contributor.authorKamber, Demir
dc.contributor.authorMithat, Evecen
dc.contributor.authorKemal, Ak
dc.date.accessioned2025-01-15T06:51:56Z
dc.date.available2025-01-15T06:51:56Z
dc.date.issued2025-01
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü
dc.description.abstractRam sperm are more vulnerable to freezing than those of most other farm animals. During sperm freezing, the cell membrane loses some of its cholesterol, which regulates signalling mechanisms and prevents premature capacitation. Resveratrol (RES) increases the fluidity of the cell membrane, which becomes peroxidized during freezing and reduces free radicals. In this study, the effectiveness of RES, cholesterol-loaded cyclodextrin (CLC) and their combinations in ram sperm cryopreservation were investigated. The collected semen was divided into two equal volumes: One was diluted with tris-citric acid-glucose medium (TCG) containing CLC, whereas the other was diluted with a CLC-free TCG solution. After examining motility, both groups were further divided into two equal volumes, forming the following working groups: control (no RES, no CLC); RES (20 µg/mL); CLC (2 mg CLC/120 × 10 sperm); and RES + CLC (RES 20 µg/mL + 2 mg CLC/120 × 10 sperm). These groups were diluted with media containing their respective additives. Post-thawing, the samples were analysed for motility, acrosome and membrane integrity, membrane functionality, mitochondrial activity, capacitation status, oxidative stress and DNA integrity. CLC preserved sperm total motility, acrosome and plasma membrane integrity and decreased the rate of early capacitation (p < 0.05). RES had no significant effect on sperm quality before freezing and post-thawing (p > 0.05). However, RES + CLC increased mitochondrial activity post-thawing (p < 0.05). In conclusion, CLC minimized sperm membrane damage caused by cryopreservation in ram sperm. RES alone was ineffective, and the combination of RES and CLC did not yield a positive synergistic effect on ram spermatological parameters.
dc.identifier.citationAhmet, E., Ramazan, A., Selin, Y., İzem, S. A., Nur, E., Kamber, D., ... & Kemal, A. (2025). The Effect of Cholesterol‐Loaded Cyclodextrin and Resveratrol Compounds on Post‐Thawing Quality of Ram Semen. Veterinary Medicine and Science, 11(1), e70172.
dc.identifier.doi10.1002/vms3.70172
dc.identifier.other39792068
dc.identifier.pmid39792068
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/vms3.70172
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8452
dc.identifier.wosqualityQ2
dc.indekslendigikaynakPubMed
dc.institutionauthorEser, Ahmet
dc.institutionauthorid0000-0003-1326-2678
dc.language.isoen
dc.publisherWiley
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcholesterol
dc.subjectcryopreservation
dc.subjectram sperm
dc.subjectresveratrol
dc.subjectspermatological examination
dc.titleThe Effect of Cholesterol-Loaded Cyclodextrin and Resveratrol Compounds on Post-Thawing Quality of Ram Semen.
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Ahmet-Eser-2024.pdf
Boyut:
366.18 KB
Biçim:
Adobe Portable Document Format
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.17 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

PubMed İndeksli Yayın Koleksiyonu
2) Makale
Scopus İndeksli Yayınlar Koleksiyonu
WOS İndeksli Yayınlar Koleksiyonu