Pyometra alters uterine aquaporins related with lipopolysaccharide concentrations and antioxidant enzyme activities in bitches

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dc.authoridTUNC, ARDA SELIN/0000-0002-4813-7626
dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.contributor.authorYazlik, Murat Onur
dc.contributor.authorOzkan, Huseyin
dc.contributor.authorVural, Sevil Atalay
dc.contributor.authorKaya, Ufuk
dc.contributor.authorMustalk, Inci Basak
dc.contributor.authorAltinbas, Ipek Mutluer
dc.contributor.authorTunc, Arda Selin
dc.date.accessioned2024-12-24T19:25:19Z
dc.date.available2024-12-24T19:25:19Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractPyometra is a common life-threatening inflammatory disease with a complex etiopathogenesis that develops during the diestrus stage and can be observed in elderly intact bitches. The present study evaluated five aquaporin (AQP1, AQP2, AQP3, AQP5, and AQP9) transcript abundances and immunolocalization in the uterine tissue, and investigated their relationship with uterine tissue and blood lipopolysaccharide (LPS) concentration, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activity, and nitric oxide (NO) production in dogs suffering from pyometra. The study sampled 36 client-owned intact bitches from different breeds, of which 24 cases were diagnosed with pyometra. Twelve of these bitches in the diestrus stage that presented for elective ovariohysterectomy were used as the control group. Blood samples were collected into tubes without anticoagulant for serum progesterone, LPS concentration, and antioxidant activities at the time of diagnosis. Bacteriological and tissue samples from the uteri were collected after the ovariohysterectomy. The tissue samples were used to determine antioxidant activity, and hor- mone and toxin concentrations. Transcript abundance of uterine AQPs were determined by qPCR, and their presence and localization were determined by by immunohistochemistry. For all pyo- metra samples, the bacteria isolated from the uterine swabs were Escherichia coli. Compared to the control group, AQP1, AQP2, and AQP5 were downregulated more than 2-fold, whereas AQP9 was upregulated nearly 3-fold and AQP3 was upregulated more than 4-fold in the pyometra affected uteri (P<0.05). Uterine AQP1 was moderately negatively correlated with serum LPS concentra- tion (r=-0.568, P<0.01) and tissue NO production (r=-0.407, P<0.05). AQP5 was positively correlated with serum SOD activity (r=0.485, P<0.05) and negatively correlated with serum LPS concentration (r=-0.512, P<0.05). AQP9 was negatively correlated with tissue SOD and serum GPx activity. This is the first study to identify AQP9 transcript abundance and immunolocaliza- tion in canine uterine tissue. Uterine AQP1, AQP2, AQP3, AQP5, and AQP9 transcript abundances were altered in spontaneously developed canine pyometra while AQP transcript abundance was negatively related to serum toxin concentration, NO production, and antioxidant enzyme activity. Further studies should be conducted to determine the role of altered abundances of AQPs tran- scripts in pyometra pathogenesis.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK), Turkiye [120O543]
dc.description.sponsorshipThis study was conducted as a project supported by the Scientific and Technological Research Council of Turkiye (TUBITAK) (Project number: 120O543) ,Turkiye.
dc.identifier.doi10.1016/j.anireprosci.2024.107621
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.pmid39471705
dc.identifier.scopus2-s2.0-85207704812
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2024.107621
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6351
dc.identifier.volume271
dc.identifier.wosWOS:001350223600001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectAquaporin
dc.subjectCanine
dc.subjectOxidative stress
dc.subjectPyometra
dc.titlePyometra alters uterine aquaporins related with lipopolysaccharide concentrations and antioxidant enzyme activities in bitches
dc.typeArticle

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