Effect of presynchronization with prostaglandin F2? before the 5-d timed AI protocol on ovarian responses and pregnancy in dairy heifers

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dc.authoridDe Souza Ribeiro, Eduardo/0000-0002-4201-7383
dc.authoridKarakaya-Bilen, Ebru/0000-0003-4837-1858
dc.authoridSantos, Jose Eduardo/0000-0003-3403-1465
dc.contributor.authorKarakaya-Bilen, Ebru
dc.contributor.authorRibeiro, Eduardo S.
dc.contributor.authorBisinotto, Rafael S.
dc.contributor.authorGumen, Ahmet
dc.contributor.authorSantos, Jose Eduardo P.
dc.date.accessioned2024-12-24T19:27:46Z
dc.date.available2024-12-24T19:27:46Z
dc.date.issued2019
dc.departmentSiirt Üniversitesi
dc.description.abstractThe objectives were the determine the effects of presynchronization with PGF(2)(alpha) 2 days before the 5-d timed artificial insemination (Al) protocol on ovarian responses and pregnancy per AI (P/AI) in dairy heifers. The hypothesis was that PGF(2)(alpha )m would induce responsive heifers to be in proestrus at the initiation of the timed Al protocol, which was expected to improve ovulatory responses and P/AI. Weekly cohorts of Holstein heifers were blocked by age and, within block, randomly assigned to remain as control (CON; n = 255) or receive PGF(2)(alpha) on experiment Day -10 (PG; n = 255). All heifers were subjected to the 5-d timed AI protocol (Day -8, GnRH + intravaginal progesterone controlled internal drug release insert; Day -3, PGF(2)(alpha) and insert removal; Day -2, PGF(2)(alpha); and Day 0, GnRH and AI). A subset of 22 blocks of heifers (n = 43) had their ovaries scanned by ultrasonography on experiment Days -8, -3, 0, and 2 and blood was sampled and analyzed for concentrations of progesterone on experiment Days -8, -7, -5, and -3. Pregnancy was diagnosed on experiment Days 32 and 60. On the day of the first GnRH of the timed AI protocol, PG heifers had smaller concentration of progesterone in plasma (CON = 4.5 +/- 0.5 vs. PG = 0.5 +/- 0.5 ng/mL), but larger follicular diameter (CON = 9.1 +/- 0.5 vs. PG = 11.0 +/- 0.5 mm), and a greater proportion of them had a follicle with at least 8.0 mm in diameter (CON = 61.9 vs. PG = 90.9%) than CON heifers, which resulted in increased ovulation to GnRH (CON = 19.0 vs. PG = 86.3%). Ovulation to the initial GnRH of the protocol increased as the concentration of progesterone in plasma decreased, from less than 20% when progesterone was greater than 5.0 ng/mL to more than 65% when progesterone was less than 1.0 ng/mL. More CON than PG heifers spontaenously ovulated before the day of timed AI. Detection of estrus on the day of timed AI did not differ between treatments (CON = 50.9 vs. PG = 46.6%), but P/AI on Days 32 (CON = 52.9 vs. PG = 61.1%) and 60 (CON = 49.0 vs. PG = 57.1%) after inseminationtended to be greater for PG than CON; however, the benefit to presynchronization was observed in heifers inseminated with conventional (CON = 54.7 vs. PG = 67.4%), but not in heifers inseminated with sex-sorted semen (CON = 50.9 vs. PG = 52.8%). Administration of PGF(2)(alpha) 2 days before initiating the timed AI protocol induced heifers to be in proestrus, which enhanced ovulation to the initial GnRH and favored pregnancy per AI, particularly in heifers inseminated with conventional semen. (C) 2019 Published by Elsevier Inc.
dc.identifier.doi10.1016/j.theriogenology.2019.03.019
dc.identifier.endpage143
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.pmid31022603
dc.identifier.scopus2-s2.0-85064843459
dc.identifier.scopusqualityQ1
dc.identifier.startpage138
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2019.03.019
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6790
dc.identifier.volume132
dc.identifier.wosWOS:000469159900017
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofTheriogenology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectDairy heifer
dc.subjectProstaglandin
dc.subjectSynchronization
dc.subjectTimed AI
dc.titleEffect of presynchronization with prostaglandin F2? before the 5-d timed AI protocol on ovarian responses and pregnancy in dairy heifers
dc.typeArticle

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