Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain

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dc.authoridBAHCIVAN, Emre/0000-0002-4415-1738
dc.contributor.authorEr, Ayse
dc.contributor.authorCoskun, Devran
dc.contributor.authorBahcivan, Emre
dc.contributor.authorDik, Burak
dc.date.accessioned2024-12-24T19:30:37Z
dc.date.available2024-12-24T19:30:37Z
dc.date.issued2020
dc.departmentSiirt Üniversitesi
dc.description.abstractObjective(s): Prevention of inflammation in early stages will be useful in maintaining vitality of the organism. The objective of this study was to evaluate the effects of doxycycline (DOX) or meloxicam (MLX) monotherapy and combination therapy on the levels of inflammatory mediators in the brain tissues of rats with Escherichia coli lipopolysaccharide (LPS)-induced brain inflammation. Materials and Methods: Seventy-eight rats were divided into the following groups: control (n=6), LPS (0.5 mu g/10 mu l intracranial) (n=18), LPS (0.5 mu g/10 mu l intracranial)+DOX (40 mg/kg intraperitoneal) (n=18), LPS (0.5 mu g/10 mu l intracranial)+MLX (2 mg/kg intraperitoneal) (n=18) and LPS (0.5 mu g/10 mu l intracranial)+DOX (40 mg/kg intraperitoneal)+MLX (2 mg/kg intraperitoneal) (n=18) groups. Brain tissues were harvested from all rats in the control group and from six rats each in the four experimental groups at 1, 3 and 6 hr under anaesthesia. The levels of tumor necrosis factor alpha (TNF alpha), interleukin 4 (IL-4), IL-6, IL-10, IL-17, brain-derived neurotrophic factor (BDNF), matrix metalloproteinase 3 (MMP-3), tissue inhibitor of metalloproteinase 3 (TIMP-3) and cyclooxygenase 2 (COX-2) in the brain tissues were measured using ELISA kits with ELISA device. Results: LPS administration increased proinflammatory cytokines (TNF, IL-6, IL-17), and MMP-3 levels and decreased anti-inflammatory cytokines (IL-10, IL-4), and BDNF levels. The lowest TNF alpha levels were detected in the LPS+MLX group (P<0.05). All the drug treatment groups showed decreased IL-17 and COX-2 levels compared to the LPS groups. Conclusion: DOX or MLX monotherapy exerts neuroprotective effects against brain inflammation by decreasing proinflammatory cytokine levels and by increasing anti-inflammatory cytokines levels.
dc.description.sponsorshipSUBAPK [17401057]
dc.description.sponsorshipThe authors thanks to Dr. Enver Yazar. This study was supported by SUBAPK (17401057). This study was presented in abstract form at 4th International Congress on Veterinary and Animal Sciences (ICVAS), Nevsehir, Turkey. Date: July 12-15, 2018.
dc.identifier.doi10.22038/ijbms.2020.45193.10527
dc.identifier.endpage1334
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.issue10
dc.identifier.pmid33149866
dc.identifier.scopus2-s2.0-85090183213
dc.identifier.scopusqualityQ2
dc.identifier.startpage1328
dc.identifier.urihttps://doi.org/10.22038/ijbms.2020.45193.10527
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7611
dc.identifier.volume23
dc.identifier.wosWOS:000575392900012
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMashhad Univ Med Sciences
dc.relation.ispartofIranian Journal of Basic Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectBrain
dc.subjectDoxycycline
dc.subjectInflammation
dc.subjectMeloxicam
dc.subjectNeuroprotective
dc.titleEffect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain
dc.typeArticle

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