Wide bovine tick-borne pathogen spectrum: Predominancy of Theileria annulata and the first molecular detection of Ehrlichia minasensis in Turkey

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dc.authoridCeylan, Ceylan/0000-0001-8072-2983
dc.contributor.authorCeylan, Onur
dc.contributor.authorMa, Zhuowei
dc.contributor.authorCeylan, Ceylan
dc.contributor.authorCulha, Muhammed Hudai
dc.contributor.authorGalon, Eloiza May
dc.contributor.authorJi, Shengwei
dc.contributor.authorLi, Hang
dc.date.accessioned2024-12-24T19:24:50Z
dc.date.available2024-12-24T19:24:50Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractVector-borne diseases indulge in severe economic losses in the livestock industry by adversely affecting cattle breeding in tropical and subtropical zone countries, including Turkey, encompassing a wide land area representing diverse climatic conditions. This study aimed to investigate significant bovine tick-borne piroplasm, rickettsia, and some other bacterial agents by genus- or species-specific PCR and nested PCR techniques in Turkey. A total of 210 cattle blood samples were collected from sixteen provinces in different geographical regions of Turkey. PCR analyses were performed targeting the detection of Babesia/Theileria/Hepatozoon sp. 18S rRNA, Babesia/Theileria sp. 18S rRNA (V4), B. bigemina RAP-1a, B. bovis SBP-4, B. ovata AMA-1, B. naoaki AMA-1, T. annulata Tams-1, T. orientalis MPSP, T. mutans 18S rRNA, Anaplasma/Ehrlichia sp. 16S rRNA, A. marginale MSP4, A. bovis 16S rRNA, A. phagocytophilum 16S rRNA, A. capra 16S rRNA, E. ruminantium pSC20, Mycoplasma sp. 16S rRNA, and Coxiella burnetii 16S rRNA genes. Overall, 133 (63.3%) cattle were found to be infected with at least one of the following protozoan or bacterial pathogens; B. bovis, B. bigemina, B. occultans, T. annulata, T. orientalis, A. marginale, A. phagocytophilum, and Mycoplasma sp. The total prevalence of pathogens was determined as follows; 0.5% B. bovis, 0.5% B. bigemina, 1.4% B. occultans, 41.0% T. annulata, 1.4% T. orientalis, 10.5% A. marginale, 13.8% A. phagocytophilum, 0.5% A. bovis, 2.9% Uncultured Anaplasma sp., 0.5% E. minasensis, 0.5% Uncultured Ehrlichia sp., and 23.3% Mycoplasma sp. Moreover, large part of the total infection (n:133) was composed of single infections (63.9%); however, double (24.8%), triple (7.5%), quadruple (2.3%), and quintuple (1.5%) co-infections were also encountered. In addition to some bovine pathogens such as B. occultans, T. orientalis, A. bovis, M. wenyonii, and Candidatus Mycoplasma haemobos, which were rarely reported in Turkey, sequencing and phylogenetic analysis revealed the first detection of Uncultured Ehrlichia sp. (0.5%), and E. minasensis (0.5%) with 100% nucleotide sequence identities. The study also indicates that the spectrum of pathogens harbored by Turkish cattle is quite wide, and these pathogens cause multiple co-infections with various combinations, and T. annulata stands out as the primary bovine pathogen among them.
dc.description.sponsorshipJapanese Society for the Promotion of Science (JSPS) Core-to-Core Program; Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan [18KK0188]
dc.description.sponsorshipThe Japanese Society for the Promotion of Science (JSPS) Core-to-Core Program and Grant-in-Aid for Scientific Research (18KK0188) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan supplied financial support for this study
dc.identifier.doi10.1007/s11259-023-10266-z
dc.identifier.endpage1059
dc.identifier.issn0165-7380
dc.identifier.issn1573-7446
dc.identifier.issue2
dc.identifier.pmid38072901
dc.identifier.scopus2-s2.0-85179349053
dc.identifier.scopusqualityQ2
dc.identifier.startpage1037
dc.identifier.urihttps://doi.org/10.1007/s11259-023-10266-z
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6138
dc.identifier.volume48
dc.identifier.wosWOS:001119656600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofVeterinary Research Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectAnaplasma bovis
dc.subjectBabesia bovis
dc.subjectBabesia occultans
dc.subjectEhrlichia minasensis
dc.subjectTheileria annulata
dc.subjectMycoplasma sp
dc.titleWide bovine tick-borne pathogen spectrum: Predominancy of Theileria annulata and the first molecular detection of Ehrlichia minasensis in Turkey
dc.typeArticle

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