Covalent immobilization of an alkaline protease from Bacillus licheniformis

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dc.contributor.authorAslan, Yakup
dc.contributor.authorÖmerosmanoğlu, Derya
dc.contributor.authorKoç, Eda Öndül
dc.date.accessioned2019-11-28T23:38:31Z
dc.date.available2019-11-28T23:38:31Z
dc.date.issuedAralık, 2018en_US
dc.departmentMühendislik Fakültesien_US
dc.description.abstractObjective: Since the soluble enzymes can not be used in repeated reactions and are not stable in operational conditions and not suitable for continuous processes, this study aimed the covalent immobilization of Bacillus licheniformis protease (BLP) onto Eupergit CM. Methods: Optimum conditions for immobilization were determined by changing the conditions individually. The proteins and L-tyrosine were determined by UV/VIS spectrophotometer. Results: The immobilization resulted in 100% immobilization and 107.7% activity yields. The optimum pH (7–8) and the optimum temperature (70°C) have not changed after immobilization. The Km values for free and immobilized enzyme were 26.53 and 37.59 g/L, while the Vmax values were 2.84 and 3.31 g L-Tyrosine/L · min, respectively. The immobilized enzyme has not lost its initial activity during the repeated 20 uses and 20 days of storage. The milk proteins were hydrolyzed in 2 h by using immobilized enzyme. The pH of the milk dropped from 6.89 to 6.53, the color was clearer but there was no change in the smell or the taste. Conclusion: Consequently, it can be said that the immobilized BLP obtained can be used for industrial purposes.en_US
dc.description.sponsorshipSiirt Üniversitesi BAP Koordinatörlüğü Proje No: 2016-SİÜFEB-03en_US
dc.identifier.doi10.1515/tjb-2017-0155
dc.identifier.endpage604en_US
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85056613292
dc.identifier.scopusqualityQ3
dc.identifier.startpage595en_US
dc.identifier.trdizinid309527
dc.identifier.urihttps://www.degruyter.com/view/j/tjb.2018.43.issue-6/tjb-2017-0155/tjb-2017-0155.xml
dc.identifier.urihttps://hdl.handle.net/20.500.12604/2067
dc.identifier.volume43en_US
dc.identifier.wosWOS:000450015000004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherDeGruyteren_US
dc.relation.ispartofTurkish Journal of Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/restrictedAccessen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.snmzKG_20241224
dc.subjectCow’s milk; Eupergit CM; Immobilization; Protease; Protein; Protein allergy.en_US
dc.titleCovalent immobilization of an alkaline protease from Bacillus licheniformisen_US
dc.title.alternativeBacillus licheniformis’ten bir alkalin proteazın kovalent immobilizasyonuen_US
dc.typeArticleen_US

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