Molecular discrimination of G1 and G3 genotypes of Echinococcus granulosus sensu stricto obtained from human, cattle, and sheep using the mitochondrial NADH dehydrogenase subunit 5 marker

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dc.authoridCao, Jianping/0000-0002-1974-0047
dc.authoridAhmed, Haroon/0000-0002-0382-3569
dc.contributor.authorCelik, Figen
dc.contributor.authorSelcuk, Muhammed Ahmed
dc.contributor.authorKilinc, Seyma Gunyakti
dc.contributor.authorKesik, Harun Kaya
dc.contributor.authorAhmed, Haroon
dc.contributor.authorWang, Ying
dc.contributor.authorSimsek, Sami
dc.date.accessioned2024-12-24T19:25:08Z
dc.date.available2024-12-24T19:25:08Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractCystic echinococcosis (CE) is a common zoonotic disease caused by the larval form of Echinococcus granulosus sensu lato. This study determined the genotype and haplotype differences using the NADH dehydrogenase subunit 5 gene in hydatid cyst samples. Human (n = 12), cattle (n = 28), and sheep (n = 31) hydatid cyst isolates were included. Seventy-one genomic DNA samples were successfully extracted, and a 759 bp mitochondrial NADH dehydrogenase subunit 5 gene fragment was amplified by PCR. Following the sequence analysis, E. granulosus sensu stricto isolates were identified as G1 (n = 61) and G3 (n = 10). A total of 23 haplotypes were obtained from the 71 E. granulosus s.s. G1 and G3 samples. The main haplotype was Hap01 (60.56 %), which consisted of the G1 genotype. The second largest haplotype was Hap04, which consisted entirely of the G3 genotype. Hap14 acted as a bridge between the G1 and G3 genotypes. This study identifies G1 as the dominant genotype in humans and farm animals in Turkey. High haplotype and nucleotide diversity in genotypes were observed. Additionally, this is the first report on the phylogeography and gene flow models of the E. granulosus s. s. population in Turkey using the NADH dehydrogenase subunit 5 gene, the best marker distinguishing between G1 and G3 genotypes.
dc.description.sponsorshipFirat University Scientific Research Project Unit [VF.22.25]; Three-Year Public Health Action Plan of Shanghai [GWV-10.1-XK13]
dc.description.sponsorshipThis study was financially supported by the Firat University Scientific Research Project Unit (No. VF.22.25 to Figen Celik) and the Three-Year Public Health Action Plan of Shanghai (No. GWV-10.1-XK13 to JC) . The funders had no role in the study design, data collection and analysis, data interpretation, decision to publish, or preparation of the manuscript.
dc.identifier.doi10.1016/j.actatropica.2024.107124
dc.identifier.issn0001-706X
dc.identifier.issn1873-6254
dc.identifier.pmid38262573
dc.identifier.scopus2-s2.0-85183653229
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.actatropica.2024.107124
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6289
dc.identifier.volume252
dc.identifier.wosWOS:001173415100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofActa Tropica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectEchinococcus granulosus sensu stricto
dc.subjectNADH dehydrogenase subunit 5
dc.subjectG1 and G3 genotypes
dc.subjectSheep
dc.subjectCattle
dc.subjectHuman
dc.titleMolecular discrimination of G1 and G3 genotypes of Echinococcus granulosus sensu stricto obtained from human, cattle, and sheep using the mitochondrial NADH dehydrogenase subunit 5 marker
dc.typeArticle

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