Genetic diversity and haplotypes of Cysticercus tenuicollis isolates from slaughtered sheep and goats in Elazig and Bingol provinces of Turkey

Basit Öğe Kaydı
dc.authoridMUHAMMED AHMED, SELCUK/0000-0003-1769-4558
dc.authoridCELIK, FIGEN/0000-0002-2188-0196
dc.authoridSimsek, Sami/0000-0002-3567-326X
dc.authoridKESIK, Harun Kaya/0000-0002-8480-8597
dc.contributor.authorKarakoc, Sirvan
dc.contributor.authorKesik, Harun Kaya
dc.contributor.authorCelik, Figen
dc.contributor.authorSelcuk, Muhammed Ahmed
dc.contributor.authorKilinc, Seyma Gunyakti
dc.contributor.authorSimsek, Sami
dc.date.accessioned2024-12-24T19:24:22Z
dc.date.available2024-12-24T19:24:22Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractBackground: The cestode Taenia hydatigena uses canids, primarily dogs, as definitive hosts, whereas the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep and pigs. Objectives: In this study, we aimed to determine the genetic differences and haplotypes of Cysticercus tenuicollis isolates obtained from sheep and goats slaughtered in the Bingol and Elazig provinces of Turkey. Methods: C. tenuicollis isolates were collected from 44 sheep and 26 goats slaughtered in slaughterhouses in Bingol and Elazig. After the isolation of total genomic DNA from C. tenuicollis isolates, the genetic characterization of the partial mitochondrial cytochrome oxidase 1 (CO1) gene region (866 bp) was amplified using specific primers by polymerase chain reaction (PCR), the products were then sequenced, and haplotype and genetic diversity analyses were carried out. Results: As a result of the haplotype network analyses, 34 different haplotypes were detected around the main haplotype (Hap02) arranged in a star-like configuration and separated from other haplotypes by 1-28 mutation steps and covering 22.85% (16/70) of all isolates. Twenty-seven polymorphic fields were detected, 77.77% (21/27) of which were parsimony-informative, and secondary haplotype and nucleotide diversity were observed. Additionally, we detected high intraspecies haplotype diversity (hd: 0.933) and high nucleotide diversity (pi: 0.00383), with 27 different nucleotide variation positions among the haplotypes of the isolates. Tajima's D value was negative, indicating population expansion and/or selection purification. The significantly negative Fu's Fs values indicated recent population expansion or the presence of expected rare haplotypes. Conclusion: The results of this study confirmed that C. tenuicollis isolates clustered in one lineage and were closely related to the relevant reference sequences in different countries, confirming the circulation of C. tenuicollis in different geographical regions.
dc.description.sponsorshipBingl niversitesi
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1002/vms3.1411
dc.identifier.issn2053-1095
dc.identifier.issue4
dc.identifier.pmid38804612
dc.identifier.scopus2-s2.0-85194523059
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/vms3.1411
dc.identifier.urihttps://hdl.handle.net/20.500.12604/5945
dc.identifier.volume10
dc.identifier.wosWOS:001233317700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofVeterinary Medicine and Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectCysticercus tenuicollis
dc.subjectsheep
dc.subjectgoat
dc.subjectmt-CO1
dc.subjectPCR
dc.subjecthaplotype
dc.titleGenetic diversity and haplotypes of Cysticercus tenuicollis isolates from slaughtered sheep and goats in Elazig and Bingol provinces of Turkey
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu