Determination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punica granatum)-A Chemical Profiling by LC-MS/MS

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dc.authoridIZOL, Ebubekir/0000-0003-0788-4999
dc.authoridGulcin, ilhami/0000-0001-5993-1668
dc.authoridKARAGECILI, HASAN/0000-0001-6912-3998
dc.contributor.authorKaragecili, Hasan
dc.contributor.authorIzol, Ebubekir
dc.contributor.authorKirecci, Ekrem
dc.contributor.authorGulcin, Ilhami
dc.date.accessioned2024-12-24T19:33:42Z
dc.date.available2024-12-24T19:33:42Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractZivzik pomegranate (Punica granatum) has recently sparked considerable interest due to its nutritional and antioxidant properties. To evaluate the antioxidant capacities of P. granatum juice, ethanol (EEZP), and water (WEZP) extracts from peel and seed, the antioxidant methods of 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS(center dot)+) scavenging, 1,1-diphenyl-2picrylhydrazyl free radical (DPPH center dot) scavenging, Fe3+-2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) reducing, Fe3+ reducing, and Cu2+ reducing methods were used. The antioxidant capacities of samples were compared with the most commonly used synthetic antioxidants, i.e., BHA, BHT, ff-tocopherol, and Trolox. In terms of setting an example, the IC50 values of EEZP for ABTS(center dot)+ and DPPH center dot scavenging activities were found to be lower than standards, at 5.9 and 16.1 mu g/mL, respectively. The phenolic and flavonoid contents in EEZP peel were 59.7 mg GAE/g and 88.0 mg QE/g, respectively. Inhibition of ff-glycosidase, ff-amylase, acetylcholinesterase, and human carbonic anhydrase II (hCA II) enzymes was also investigated. EEZP demonstrated IC50 values of 7.3 mu g/mL against ff-glycosidase, 317.7 mu g/mL against ff-amylase, 19.7 mu g/mL against acetylcholinesterase (AChE), and 106.3 mu g/mL against CA II enzymes. A total of 53 phenolic compounds were scanned, and 30 compounds were determined using LC-MS/MS. E. coli and S. aureus bacteria were resistant to all four antibiotics used as standards in hospitals.
dc.description.sponsorshipScientific Research Projects Coordination at Siirt University [2021-SUSBF-040]
dc.description.sponsorshipScientific Research Projects Coordination at Siirt University (2021-SUSBF-040) provided funding for this study.
dc.identifier.doi10.3390/life13030735
dc.identifier.issn2075-1729
dc.identifier.issue3
dc.identifier.pmid36983890
dc.identifier.scopus2-s2.0-85151382113
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/life13030735
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8226
dc.identifier.volume13
dc.identifier.wosWOS:000960506700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofLife-Basel
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectZivzik pomegranate
dc.subjectenzyme inhibition
dc.subjectPunica granatum
dc.subjectantioxidant
dc.subjectalpha-glycosidase
dc.subjectacetylcholinesterase
dc.subjectcarbonic anhydrase
dc.subjectLC-MS/MS analysis
dc.titleDetermination of Antioxidant, Anti-Alzheimer, Antidiabetic, Antiglaucoma and Antimicrobial Effects of Zivzik Pomegranate (Punica granatum)-A Chemical Profiling by LC-MS/MS
dc.typeArticle

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