Prevalence and Subtypes Distribution (ST10, ST14, ST25, ST26) of Blastocystis spp. in Anatolian Water Buffalo (Bubalus bubalis) in Van, Turkiye

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dc.contributor.authorAyan, Adnan
dc.contributor.authorCelik, Burcak Aslan
dc.contributor.authorCelik, Ozgur Yasar
dc.contributor.authorYilmaz, Ali Bilgin
dc.contributor.authorKilinc, Ozlem Orunc
dc.contributor.authorAyan, Ozge Oktay
dc.date.accessioned2024-12-24T19:24:22Z
dc.date.available2024-12-24T19:24:22Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractBackground: Blastocystis spp. is one of the most common protozoa worldwide, living in the gastrointestinal tract of humans and many other animals. On the basis of the genetic heterogeneity of small subunit ribosomal RNA, at least 28 subtypes (ST1-ST17, ST21 and ST23-ST32) are reported to exist in mammals and birds. ObjectivesThis study was carried out to determine the prevalence and subtypes of Blastocystis spp. in Anatolian buffaloes (Bubalus bubalis) in Van province in the Eastern Anatolia Region of Turkey. Methods: DNA was extracted using commercial GeneMATRIX Stool DNA Purification Kit and then stored at -20 degrees C until PCR amplification. After PCR amplification of the SSU rRNA gene region positive Blastocystis spp., amplicons from buffalo faeces were sequenced and then deposited in GenBank (OR576949.1, OR576950.1, OR576970.1, OR576971.1, OR577019.1, PP837943.1, PP837940.1, PP837939.1, PP837604.1, PP837937.1, PP837934.1, PP837601.1, PP837936.1 and PP837603.1). Results: PCR analysis of 120 faecal samples showed a total prevalence of 11.67% (14/120). The prevalence was higher in females and young animals (p > 0.05). Sequence analysis revealed Blastocystis spp., ST10, ST14, ST25 and ST26 subtypes. To our knowledge, Blastocystis subtypes ST25 and ST26 in buffaloes were reported for the first time in this study. Conclusion: It is thought that more large-scale studies should be carried out to determine the zoonotic subtype potential of this protozoan in the region.
dc.identifier.doi10.1002/vms3.70054
dc.identifier.issn2053-1095
dc.identifier.issue6
dc.identifier.pmid39329238
dc.identifier.scopus2-s2.0-85205151581
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/vms3.70054
dc.identifier.urihttps://hdl.handle.net/20.500.12604/5952
dc.identifier.volume10
dc.identifier.wosWOS:001321157200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofVeterinary Medicine and Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectAnatolian buffalo
dc.subjectblastocystis subtypes
dc.subjectT & uuml;rkiye
dc.subjectVan
dc.titlePrevalence and Subtypes Distribution (ST10, ST14, ST25, ST26) of Blastocystis spp. in Anatolian Water Buffalo (Bubalus bubalis) in Van, Turkiye
dc.typeArticle

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