Cultural conditions optimization for production of β-galactosidase from Bacillus licheniformis ATCC 12759 under solid-state fermentation
Yükleniyor...
Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: The aim of this work was to study the optimal
cultivation conditions for β-galactosidase production by
Bacillus licheniformis ATCC 12759.
Materials and methods: The screening of β-galactosidase
production from B. licheniformis ATCC 12759 was performed
by solid state fermentation method on media rich
with rice bran (RB). Different factors were tested for the
optimization of β-galactosidase production.
Results: Certain fermentation parameters involving
incubation time, incubation temperature, inoculum
level, moisture content, initial pH, agitation speed, size
of fermentation medium and optimum temperature of
β-galactosidase activity were studied separately. Maximal
amount of β-galactosidase production was obtained when
solid-state fermentation (SSF) was carried out using RB,
having inoculum level 35%, moisture content of 20%,
initial pH 7.5 at 37°C for 48 h.
Conclusion: Results indicated that optimal fermentation
conditions play a key role in the maximum production
of β-galactosidase from B. licheniformis ATCC 12759. This
study shows the potential of the studied enzymes to be
promoting candidates for the degradation of lactose and
production of important bioproducts.
Açıklama
Anahtar Kelimeler
Bacillus licheniformis; β-galactosidase; Fermentation; Optimization; Rice bran.
Kaynak
Turkish Journal of Biochemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
43
Sayı
3
