Evaluation of endothelial glycocalyx injury biomarkers in feline hemotropic mycoplasmosis

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dc.authoridCeylan, Ceylan/0000-0001-8072-2983
dc.contributor.authorIder, Merve
dc.contributor.authorCeylan, Ceylan
dc.contributor.authorNaseri, Amir
dc.contributor.authorCeylan, Onur
dc.contributor.authorDurgut, Murat Kaan
dc.contributor.authorOk, Mahmut
dc.contributor.authorIyigun, Suleyman Serhat
dc.date.accessioned2024-12-24T19:27:58Z
dc.date.available2024-12-24T19:27:58Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractThe present study aimed to investigate endothelial glycocalyx (eGCx) damage in cats with feline hemotropic mycoplasmosis caused by Mycoplasma haemofelis using selected biomarkers and to determine the diagnostic and prognostic significance of these biomarkers. The study included 25 cats with feline hemotropic mycoplasmosis and 10 healthy cats. Clinical examination, blood gas analysis, complete blood count, and biochemical analysis were performed. Hemotropic mycoplasmosis diagnosed by microscopic examination and molecularly confirmed by PCR targeting the Mycoplasma haemofelis 16s rRNA gene. To evaluate endothelial glycocalyx damage, syndecan-1, endothelin-1 (ET-1), asymmetric dimethylarginine (ADMA), and vascular endothelial growth factor-A (VEGF-A) concentrations were measured using cat-specific commercial ELISA kits. Of the cats with feline hemotropic mycoplasmosis, 14 (56%) survived and 11 (44%) died. While syndecan-1 and ET-1 concentrations were significantly higher in cats with hemotropic mycoplasmosis compared to the control group (p < 0.001), no statistically significant difference was found for ADMA and VEGF-A concentrations (p > 0.05). Endothelial glycocalyx biomarkers showed significant correlations with each other and with hematological parameters (p < 0.01). The results of the ROC analysis showed that ET-1 with area under the curve (AUC) of 0.821 (p < 0.01) and VEGF-A with AUC of 0.805 (p < 0.010) were found to be significant prognostic indicators. In conclusion, this study demonstrated that serum syndecan-1 and ET-1 can be used as diagnostic and serum ET-1 and VEGF-A as prognostic biomarkers in cats with hemotropic mycoplasmosis. Our results indicate the development of eGCx damage in feline hemotropic mycoplasmosis and suggest that glycocalyx disruption may contribute to the pathogenesis of the disease.
dc.description.sponsorshipSelcuk University Scientific Research Project Office
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1038/s41598-024-62359-7
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid38839816
dc.identifier.scopus2-s2.0-85195355801
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1038/s41598-024-62359-7
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6855
dc.identifier.volume14
dc.identifier.wosWOS:001244399200012
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectBiomarker
dc.subjectCat
dc.subjectEndothelin-1
dc.subjectGlycocalyx
dc.subjectMycoplasma haemofelis
dc.subjectSyndecan-1
dc.titleEvaluation of endothelial glycocalyx injury biomarkers in feline hemotropic mycoplasmosis
dc.typeArticle

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