Evaluation of the use of L-carnosine in short-term storage of ram semen with spermatological and flow-cytometric analyzes

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dc.contributor.authorGüngör, İ.H.
dc.contributor.authorGür, S.
dc.contributor.authorKoca, R.H.
dc.contributor.authorCihangiroğlu, A. Çakır
dc.contributor.authorEkmen, E. Güler
dc.contributor.authorAcısu, T.C.
dc.contributor.authorBadıllı, N.
dc.date.accessioned2024-12-24T19:10:07Z
dc.date.available2024-12-24T19:10:07Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractThis study was conducted to investigate the effect of adding different doses of L-carnosine to semen in vitro on the short-term storage of semen. Semen taken from 6 Akkaraman rams was used in the research. Semen taken from each animal was pooled. It was diluted with tris+egg yolk extender containing L-carnosine at different doses (1, 5, 10, 20, 50, 100 and 200 mM) and stored at +4°C for 96 hours. Motility and kinematic parameters, abnormal spermatozoa ratio, HOS (Hypo-osmotic swelling test), pH and flow-cytometric analyzes were performed in all experimental groups every 24 hours (0, 24, 48, 72 and 96 hours) during storage. Oxidative stress parameters were determined at 0 and 96 hours. It was determined that 1, 5, 10, 20 mM L-carnosine doses increased total, progressive and rapid motility, kinematic parameters VCL (Curvilinear velocity), VSL (Straight line velocity), VAP (Average path velocity) values and high mitochondrial membrane potential level during short-term storage. It was also determined that these doses protected membrane integrity, reduced the rate of abnormal spermatozoon, acrosomal damage and low mitochondrial membrane potential level. It was determined that a dose of 200 mM L-carnosine had a toxic effect and negatively affected the parameters [total and progressive motility, VCL, VSL, VAP, ALH (Amplitude of lateral head displacement), HOS Test, viability, acrosomal damage, HMMP (High Mitochondrial Membrane Potential), LMMP (Low Mitochondrial Membrane Potential)]. In conclusion, high doses of L-carnosine (200 mM) in semen extenders are harmful to ram semen characteristics after a short time storage. Moreover, low doses of L-carnosine (1, 5, 10 and 20 mM) supplementation may have beneficial effect on ram semen traits, depending on the time storage. © 2024, İH Güngör, S Gür, RH Koca, A Çakır Cihangiroğlu, E Güler Ekmen, TC Acısu, N Badıllı, Ş Özer Kaya, A Yüce, M Sönmez, G Türk
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK
dc.identifier.doi10.12681/jhvms.36212
dc.identifier.endpage7992
dc.identifier.issn1792-2720
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85205798032
dc.identifier.scopusqualityQ4
dc.identifier.startpage7977
dc.identifier.urihttps://doi.org10.12681/jhvms.36212
dc.identifier.urihttps://hdl.handle.net/20.500.12604/3934
dc.identifier.volume75
dc.identifier.wosqualityQ4
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherHellenic Veterinary Medical Society
dc.relation.ispartofJournal of the Hellenic Veterinary Medical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectCooling
dc.subjectFlow-cytometric analysis
dc.subjectL-carnosine
dc.subjectRam
dc.subjectSemen
dc.titleEvaluation of the use of L-carnosine in short-term storage of ram semen with spermatological and flow-cytometric analyzes
dc.typeArticle

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