Oxidative stress, apoptosis, inflammation, and proliferation modulator function of visnagin provide gonadoprotective activity in testicular ischemia-reperfusion injury

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dc.contributor.authorSagir, S.
dc.contributor.authorSeker, U.
dc.contributor.authorOzoner, M. Pekince
dc.contributor.authorYuksel, M.
dc.contributor.authorDemir, M.
dc.date.accessioned2024-12-24T19:33:23Z
dc.date.available2024-12-24T19:33:23Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractOBJECTIVE: Visnagin (Vis) is a compound found in the flowers and seeds of the Ammi visnaga plant with promising antioxidant and anti-inflammatory properties. We aimed to investigate the dose-dependent gonadoprotective effects of visnagin in rats while considering oxidative stress, apoptosis, and inflammation-related protein expression levels.MATERIALS AND METHODS: Twenty-eight adult rats were divided into four groups of seven animals each; control, ischemia/reperfusion (I/R), I/R+30Vis, and I/R+60Vis. Animals in control received no surgical application and were sacrificed at the end of the experiment. The ratsin I/R, I/R + Vis30, and I/R + Vis60 were exposed to testicular ischemia and the animals in I/R + Vis30, and I/R + Vis60 groups received either 30 or 60 mg/kg visnagin intraperitoneal. At the end of the experiment, testis tissues were used for the measurement of oxidative stress, apoptosis, and inflammation.RESULTS: Our microscopic examinations indicated that I/R resulted in testicular degenerations and morphological alterations, which were improved in visnagintreated animals. The bio-chemical analyses demonstrated that oxidative stress in the I/R group increased significantly (p<0.05) compared to the control group. The immunohistochemical examinations showed that pro-apoptotic Bax and Caspase 3 expressions, and pro-inflammatory tumor necrosis factor -al-pha (TNF-alpha) levels were significantly up -regulated (p<0.05) but proliferating nuclear anti-gen (PCNA) levels in I/R group was significantly (p<0.001) down-regulated compared to the con-trol group.CONCLUSIONS: Ischemia leading to testicular torsion is a reproductive health-affecting problem, and current surgical treatment methods might be insufficient to recover the testis due to the accumulation of reactive oxygen species (ROS). Our observations indicate that visnagin is a potential co-modality along with the surgical interventions for the recovery of ischemia encountered testis, but we believe the requirement of more detailed studies to explore the underlying signaling pathways and the strength of visnagin against testicular ischemia-reperfusion injury.
dc.identifier.endpage9977
dc.identifier.issn1128-3602
dc.identifier.issue20
dc.identifier.pmid37916367
dc.identifier.scopus2-s2.0-85175769914
dc.identifier.scopusqualityQ2
dc.identifier.startpage9968
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8123
dc.identifier.volume27
dc.identifier.wosWOS:001107293500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherVerduci Publisher
dc.relation.ispartofEuropean Review For Medical and Pharmacological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectVisnagin
dc.subjectIschemia/reperfusion
dc.subjectApoptosis
dc.subjectInflammation
dc.subjectOxidative stress
dc.titleOxidative stress, apoptosis, inflammation, and proliferation modulator function of visnagin provide gonadoprotective activity in testicular ischemia-reperfusion injury
dc.typeArticle

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