Is there a relation between genetic susceptibility with cancer? A study about paraoxanase (PON1) enzyme activity in breast cancer cases

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dc.authoridKAYA, Mustafa Oguzhan/0000-0002-8592-1567
dc.authoridSinan, Selma/0000-0002-0632-3658
dc.contributor.authorKaya, Mustafa Oguzhan
dc.contributor.authorSinan, Selma
dc.contributor.authorGuler, Ozen Ozensoy
dc.contributor.authorArslan, Oktay
dc.date.accessioned2024-12-24T19:30:59Z
dc.date.available2024-12-24T19:30:59Z
dc.date.issued2016
dc.departmentSiirt Üniversitesi
dc.description.abstractHuman serum paraoxonase 1 (PON1, EC 3.1.1.2) is a high density lipoprotein (HDL)-associated antioxidant enzyme that not only decreases oxidative stress, but it is also implicated in development of many cancers. Genetic information provides a means of identifying people who have an increased risk of cancer, thus this knowledge of cancer genetics helps to identify the ability to characterize malignancies leading to the development of new therapeutic approaches. Because of this reason, in this preliminary study we aimed to investigate the role of human serum PON1 enzyme activity and phenotypic distribution in 32 breast cancer (BC) patients (age range 28-82) and 35 cancer free (CF) control group (age range 21-67). PON1 enzyme was prepared from the serum pool of BC patients using hydrophobic interaction chromatography on L-tyrosine-9-aminophenanthrene-coupled Sepharose 4Bgel. The PON1 enzyme activity towards paraoxon substrate was quantified spectrophotometrically. The basal activity of PON1 was statistically decreased in cancer cases compared to the control group. In addition, individuals were classified according to phenotyping of human PON1 Q and R types. In the cohort of BC patients, an increase in the frequency of the PON homozygote Q (AA) genotype was observed (31% in the BC group versus 14% in the CF controls). The frequency of the PON heterozygote QR (AB) genotype was 34.5% in the patients with BC and 37% in the CF group. The same trend was observed in PON homozygote R (BB) genotype frequency (BC cases 34.5% versus controls 49%). We determined that the kinetic parameters of the purified enzyme by Lineweaver-Burk method. We obtained K-m and V-max values of 0.227mM and 62 U/mL min for the BC enzyme, compared with 0.775 mM and 206 U/mL min for the CF control enzyme. As a conclusion, it is clear from our results that while the PON1 AA allele frequency in BC cases is much higher, that of BB allele is much lower, in comparison with the control group. The most significant finding of this study is AA allele activity which is low in BC cases was found high. We concluded that decreased AA allele PON1 activity might have a relation with BC.
dc.description.sponsorshipBalikesir University Scientific Research Foundation [BAP 2008/31]
dc.description.sponsorshipThis study was supported by the Balikesir University Scientific Research Foundation (BAP 2008/31).
dc.identifier.doi10.3109/14756366.2015.1134523
dc.identifier.endpage1355
dc.identifier.issn1475-6366
dc.identifier.issn1475-6374
dc.identifier.issue6
dc.identifier.pmid26763308
dc.identifier.scopus2-s2.0-84954218114
dc.identifier.scopusqualityQ1
dc.identifier.startpage1349
dc.identifier.urihttps://doi.org/10.3109/14756366.2015.1134523
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7757
dc.identifier.volume31
dc.identifier.wosWOS:000385270300062
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofJournal of Enzyme Inhibition and Medicinal Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectBreast cancer
dc.subjectparaoxonase
dc.subjectpolymorphism
dc.subjectpurification
dc.titleIs there a relation between genetic susceptibility with cancer? A study about paraoxanase (PON1) enzyme activity in breast cancer cases
dc.typeArticle

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