Genome-wide identification of salinity responsive HSP70s in common bean

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dc.authoridBuyuk, Ilker/0000-0002-0843-8299
dc.authoridILHAN, EMRE/0000-0002-8404-7900
dc.contributor.authorBuyuk, Ilker
dc.contributor.authorInal, Behcet
dc.contributor.authorIlhan, Emre
dc.contributor.authorTanriseven, Mehmet
dc.contributor.authorAras, Sumer
dc.contributor.authorErayman, Mustafa
dc.date.accessioned2024-12-24T19:24:42Z
dc.date.available2024-12-24T19:24:42Z
dc.date.issued2016
dc.departmentSiirt Üniversitesi
dc.description.abstractThe present study is aimed to identify and characterize HSP70 (PvHSP70) genes in two different common bean cultivars under salt stress. For this purpose various in silico methods such as RNAseq data and qRT-PCR analysis were used. A total of 24 candidate PvHSP70 gene were identified. Except for chromosome 4 and 7, these candidate PvHSP70 genes were distributed on the remaining chromosomes. While the lowest number of PvHSP70 genes was determined on chromosomes 1, 3, 5, 7, 9, 10 and 11 (one HSP70 gene), the highest number of PvHSP70s was on chromosomes 6 and 8 (seven HSP70 genes each). Three genes; PvHSP70-5, -9, and -10 were found to have no-introns. In addition, four tandemly and six segmentally duplicated gene couples were detected. A total of 13 PvHSP70 genes were targeted by miRNAs of 44 plant species and the most targeted genes were PvHSP70-5 and -23. The expression profile of PvHSP70 genes based on publicly available RNA-seq data was identified and salt treated leaf tissue was found to have more gene expression levels compared to the root. qRT-PCR analysis showed that the transcript concentrations of upregulated PvHSP70 genes in leaves of Zulbiye (sensitive) were mostly higher than those of Yakutiye (resistant). The present study revealed that PvHSP70 genes might play an important role in salt stress response for common bean cultivars and variability between cultivars also suggests that these genes could be used as functional markers for salt tolerance in common bean.
dc.identifier.doi10.1007/s11033-016-4057-0
dc.identifier.endpage1266
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue11
dc.identifier.pmid27558093
dc.identifier.scopus2-s2.0-84983375998
dc.identifier.scopusqualityQ2
dc.identifier.startpage1251
dc.identifier.urihttps://doi.org/10.1007/s11033-016-4057-0
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6103
dc.identifier.volume43
dc.identifier.wosWOS:000387671100008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectHSP70
dc.subjectGenome-wide analysis
dc.subjectRNAseq
dc.subjectqRT-PCR
dc.subjectSalt stress
dc.subjectPhaseolus vulgaris
dc.titleGenome-wide identification of salinity responsive HSP70s in common bean
dc.typeArticle

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