Bryonia multiflora Extract Induces Autophagy via Regulating Long Non-coding RNAs in Breast Cancer Cells

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dc.authoridTOKGUN, ONUR/0000-0003-0537-9032
dc.authoridTurel, Samet/0000-0003-2282-5938
dc.authoridinci, kubilay/0000-0001-9341-7945
dc.authoridTAN, Secil/0000-0003-1104-7253
dc.authoridTOKGUN, Pervin Elvan/0000-0001-9025-4140
dc.authoridINAL, Behcet/0000-0003-2215-2710
dc.authoridALVUR, Ozge/0000-0002-9802-9404
dc.contributor.authorTokgun, Onur
dc.contributor.authorTokgun, Pervin Elvan
dc.contributor.authorTurel, Samet
dc.contributor.authorInal, Behcet
dc.contributor.authorInci, Kubilay
dc.contributor.authorTan, Secil
dc.contributor.authorCan Alvur, Ozge
dc.date.accessioned2024-12-24T19:28:04Z
dc.date.available2024-12-24T19:28:04Z
dc.date.issued2021
dc.departmentSiirt Üniversitesi
dc.description.abstractBryonia multiflora, one of the species of Bryonia L. (Cucurbitaceae) genus, is a perennial, dioecious, herbaceous plant with rhizome-shaped roots. Bryonia species have anti-inflammatory, antimicrobial, cytotoxic, antioxidant, etc., activities and their components consume antitumoral effects. Purpose of the study to investigate the effect of Bryonia Multiflora extract (BMST) on breast cancer cells. Our results revealed that MCF-7 and MDA-MB-231 cells underwent significant morphological changes leading to cell rounding. No significant changes were observed in the cell viability by MTT. Acridine orange staining of our cells gave rise to think that BMST might lead our cells to autophagy. Therefore, possible molecular mechanisms underlying morphological changes such as autophagy (LC-3B, Beclin, AMBRA1) and apoptosis (Bcl-2) were evaluated on mRNA and protein levels. BMST treated MCF-7 and MDA-MB-231 cells had increased levels of autophagy markers whereas decreased levels of Bcl-2. p21 levels were also found to be increased in both cells. Analysis of lncRNA expressions has shown that BMST treatment led to changes in the expression levels of several lncRNAs playing roles in autophagy. The current study has shown that BMST induces autophagy in MCF-7 and MDA-MB-231 cells via regulating the lncRNAs revealing that BMST could be a promising therapeutic agent.
dc.identifier.doi10.1080/01635581.2021.1922717
dc.identifier.endpage1803
dc.identifier.issn0163-5581
dc.identifier.issn1532-7914
dc.identifier.issue9
dc.identifier.pmid34024207
dc.identifier.scopus2-s2.0-85106329170
dc.identifier.scopusqualityQ1
dc.identifier.startpage1792
dc.identifier.urihttps://doi.org/10.1080/01635581.2021.1922717
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6910
dc.identifier.volume73
dc.identifier.wosWOS:000653133200001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherRoutledge Journals, Taylor & Francis Ltd
dc.relation.ispartofNutrition and Cancer-An International Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.titleBryonia multiflora Extract Induces Autophagy via Regulating Long Non-coding RNAs in Breast Cancer Cells
dc.typeArticle

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