The Investigation of Giardiasis (Foodborne and Waterborne Diseases) in Buffaloes in Van Region, Turkiye: First Molecular Report of Giardia duodenalis Assemblage B from Buffaloes

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dc.authoridYuksek, Nazmi/0000-0003-4613-9334
dc.authoridAkyildiz, Gurkan/0000-0002-8610-5174
dc.contributor.authorKilinc, Ozlem Orunc
dc.contributor.authorAyan, Adnan
dc.contributor.authorCelik, Burcak Aslan
dc.contributor.authorCelik, Ozgur Yasar
dc.contributor.authorYuksek, Nazmi
dc.contributor.authorAkyildiz, Gurkan
dc.contributor.authorOguz, Fatma Ertas
dc.date.accessioned2024-12-24T19:33:43Z
dc.date.available2024-12-24T19:33:43Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractGiardia duodenalis (G. duodenalis) is an important zoonotic protozoan agent that causes foodborne and waterborne diarrhea in humans and other mammals. Molecular-based tests are critical in diagnosing giardiasis in humans and animals, identifying species, understanding the zoonotic potential and transmission routes, and evaluating taxonomy. Therefore, this study aimed to investigate the molecular characterization of G. duodenalis in buffaloes in the Van region in Turkiye. Buffaloes are a species that has been poorly studied in this regard. For this purpose, 100 fecal samples were collected from buffaloes in the Van region. The DNA extraction was performed using the GeneMATRIX STOOL DNA Purification Kit from stool samples. The nested PCR test was performed with the appropriate primers from the obtained DNA samples. The obtained bands suitable for sequencing were sent for sequence analysis, and the sequence results were aligned bidirectionally and compared with the database of GenBank by BLAST. As a result of the study, an 11% positivity rate for G. duodenalis was found in buffaloes, and assemblage E and assemblage B were isolated. To our knowledge, assemblage B in buffaloes was reported for the first time in this study. As a result, it was concluded that buffaloes are an important reservoir for waterborne and foodborne giardiasis.
dc.description.sponsorshipVan Yuzuncu Yil University Scientific Research Projects Presidency [THD-2021-9327]
dc.description.sponsorshipThis research was supported by Van Yuzuncu Yil University Scientific Research Projects Presidency (project no: THD-2021-9327).
dc.identifier.doi10.3390/pathogens12010106
dc.identifier.issn2076-0817
dc.identifier.issue1
dc.identifier.pmid36678454
dc.identifier.scopus2-s2.0-85146797347
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3390/pathogens12010106
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8255
dc.identifier.volume12
dc.identifier.wosWOS:000916367600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofPathogens
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectassemblage B
dc.subjectbuffalo
dc.subjectfoodborne
dc.subjectGiardia duodenalis
dc.subjectfirst report
dc.titleThe Investigation of Giardiasis (Foodborne and Waterborne Diseases) in Buffaloes in Van Region, Turkiye: First Molecular Report of Giardia duodenalis Assemblage B from Buffaloes
dc.typeArticle

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