Comprehensive Metabolite Profiling of Berdav Propolis Using LC-MS/MS: Determination of Antioxidant, Anticholinergic, Antiglaucoma, and Antidiabetic Effects

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dc.authoridGUVEN, LEYLA/0000-0002-3189-6415
dc.authoridGulcin, ilhami/0000-0001-5993-1668
dc.authoridYILMAZ, Mustafa Abdullah/0000-0002-4090-7227
dc.authoridKARAGECILI, HASAN/0000-0001-6912-3998
dc.contributor.authorKaragecili, Hasan
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorErturk, Adem
dc.contributor.authorKiziltas, Hatice
dc.contributor.authorGuven, Leyla
dc.contributor.authorAlwasel, Saleh H.
dc.contributor.authorGulcin, Ilhami
dc.date.accessioned2024-12-24T19:33:43Z
dc.date.available2024-12-24T19:33:43Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractPropolis is a complex natural compound that honeybees obtain from plants and contributes to hive safety. It is rich in phenolic and flavonoid compounds, which contain antioxidant, antimicrobial, and anticancer properties. In this study, the chemical composition and antioxidant activities of propolis were investigated; ABTS(center dot+), DPPH center dot and DMPD center dot+ were prepared using radical scavenging antioxidant methods. The phenolic and flavonoid contents of propolis were 53 mg of gallic acid equivalent (GAE)/g and 170.164 mg of quercetin equivalent (QE)/g, respectively. The ferric ion (Fe3+) reduction, CUPRAC and FRAP reduction capacities were also studied. The antioxidant and reducing capacities of propolis were compared with those of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol and Trolox reference standards. The half maximal inhibition concentration (IC50) values of propolis for ABTS(center dot+), DPPH center dot and DMPD center dot+ scavenging activities were found to be 8.15, 20.55 and 86.64 mu g/mL, respectively. Propolis extract demonstrated IC50 values of 3.7, 3.4 and 19.6 mu g/mL against alpha-glycosidase, acetylcholinesterase (AChE) and carbonic anhydrase II (hCA II) enzyme, respectively. These enzymes' inhibition was associated with diabetes, Alzheimer's disease (AD) and glaucoma. The reducing power, antioxidant activity and enzyme inhibition capacity of propolis extract were comparable to those demonstrated by the standards. Twenty-eight phenolic compounds, including acacetin, caffeic acid, p-coumaric acid, naringenin, chrysin, quinic acid, quercetin, and ferulic acid, were determined by LC-MS/MS to be major organic compounds in propolis. The polyphenolic antioxidant-rich content of the ethanol extract of propolis appears to be a natural product that can be used in the treatment of diabetes, AD, glaucoma, epilepsy, and cancerous diseases.
dc.description.sponsorshipTUEBA; King Saud University's Researchers [RSP-2023/59]
dc.description.sponsorshipIlhami Gulcin is a member of the Turkish Academy of Sciences (TUEBA). He wants to express his heartfelt gratitude to the TUEBA for their financial assistance. In addition, Saleh Alwasel wishes to express his heartfelt gratitude to King Saud University's Researchers Supporting Project (RSP-2023/59).
dc.identifier.doi10.3390/molecules28041739
dc.identifier.issn1420-3049
dc.identifier.issue4
dc.identifier.pmid36838726
dc.identifier.scopus2-s2.0-85149052865
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/molecules28041739
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8250
dc.identifier.volume28
dc.identifier.wosWOS:000941732200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofMolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectpropolis
dc.subjectantioxidant activity
dc.subjectalpha-glycosidase
dc.subjectacetylcholinesterase
dc.subjectcarbonic anhydrase
dc.subjectphenolic compound
dc.titleComprehensive Metabolite Profiling of Berdav Propolis Using LC-MS/MS: Determination of Antioxidant, Anticholinergic, Antiglaucoma, and Antidiabetic Effects
dc.typeArticle

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