Inhibitory properties of some heavy metals on carbonic anhydrase I and II isozymes activities purified from Van Lake fish (Chalcalburnus Tarichi) gill

Basit Öğe Kaydı
dc.authoridKUZU, Muslum/0000-0002-1375-7673
dc.contributor.authorKuzu, Muslum
dc.contributor.authorComakli, Veysel
dc.contributor.authorAkkemik, Ebru
dc.contributor.authorCiftci, Mehmet
dc.contributor.authorKufrevioglu, Omer Irfan
dc.date.accessioned2024-12-24T19:24:37Z
dc.date.available2024-12-24T19:24:37Z
dc.date.issued2018
dc.departmentSiirt Üniversitesi
dc.description.abstractIn this study, CA I and II isoenzymes were purified from Van Lake fish gills by using Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography and to determine the effects of some metals on the enzyme activities. For purified CA I isoenzyme, yield, specific activity, and purification fold were obtained as 42.07%, 4948.12 EU/mg protein, and 116.61 and for CA II isoenzyme, 7%, 1798.56 EU/mg protein, and 42.38 respectively. Activity of CA was determined by measuring CO2-hydratase activity. Purity control was checked by SDS-PAGE. In vitro inhibitory effect of Cu2+, Ag+, Cd2+, Ni2+ metal ions, and arsenic (V) oxide were also examined for both isozymes activities. Whereas Cu2+, Ag+, Cd2+, and Ni2+ ions showed inhibitory effects on both isozymes, arsenic (V) oxide showed activation effect. IC50 values were calculated by drawing activity %-[I] graphs for metal ions exhibiting inhibitory effects. IC50 values were determined as 3.39, 6.38, 13.52, and 206 mu M for CA I isozyme and 6.16, 20.29, 46, and 223 mu M for CA II isozyme respectively.
dc.identifier.doi10.1007/s10695-018-0499-8
dc.identifier.endpage1125
dc.identifier.issn0920-1742
dc.identifier.issn1573-5168
dc.identifier.issue4
dc.identifier.pmid29629489
dc.identifier.scopus2-s2.0-85045109315
dc.identifier.scopusqualityQ1
dc.identifier.startpage1119
dc.identifier.urihttps://doi.org/10.1007/s10695-018-0499-8
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6052
dc.identifier.volume44
dc.identifier.wosWOS:000437000200010
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofFish Physiology and Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectCarbonic anhydrase
dc.subjectHeavy metal
dc.subjectInhibition
dc.subjectPurification
dc.titleInhibitory properties of some heavy metals on carbonic anhydrase I and II isozymes activities purified from Van Lake fish (Chalcalburnus Tarichi) gill
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu