Protective Effects of Boron on Cyclophosphamide-Induced Bladder Damage and Oxidative Stress in Rats

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dc.authoridAppak-Baskoy, Sila/0000-0001-6105-1408
dc.authoridCENGIZ, Mustafa/0000-0002-6925-8371
dc.contributor.authorAyhanci, Adnan
dc.contributor.authorTanriverdi, Dondu Tugce
dc.contributor.authorSahinturk, Varol
dc.contributor.authorCengiz, Mustafa
dc.contributor.authorAppak-Baskoy, Sila
dc.contributor.authorSahin, Ilknur Kulcanay
dc.date.accessioned2024-12-24T19:24:54Z
dc.date.available2024-12-24T19:24:54Z
dc.date.issued2020
dc.departmentSiirt Üniversitesi
dc.description.abstractThis study aims to investigate protective effects of boron against cyclophosphamide-induced bladder toxicity that produces oxidative stress and leads to apoptosis of the cells. In total, 24 rats were divided into 4 equal groups. The control group received saline. The 2nd experimental group received 200 mg kg of cyclophosphamide i.p. on the 4th day while the 3rd group was given only boron (200 mg kg, i.p.) for 6 days. In the 4th group, boron was given for 6 days and cyclophosphamide (200 mg kg, i.p.) was administrated on the 4th day. Twenty-four hours after the last boron or cyclophosphamide administration, rats were sacrificed under anesthesia. Bladder tissues of rats were taken for histological and immunohistochemical (apoptotic markers such as caspase-3, bcl-2, and bax) and blood was taken for the biochemical (serum total thiol, serum natural thiol, serum thiol-disulfide) analysis. Transient epithelial thinning, edema, marked inflammatory reaction, and bleeding were observed in bladders of the group that received cyclophosphamide. Also, the activity of bax and caspase-3-positive cells increased while the number of bcl-2-positive cells decreased. In the same group, serum natural thiol and total thiol levels decreased while serum disulfide levels increased, which indicates oxidative stress. On the other hand, in the boron+cyclophosphamide group pretreatment with boron protected, the bladder tissue and the number of bcl-2-positive cells increased, and bax and caspase-3-positive cells decreased, showing antiapoptotic effects of boron against cyclophosphamide-induced toxicity. In parallel with the findings of this group, native thiol and total thiol levels increased and serum disulfide levels decreased pointing out to a decreased oxidative stress. Our results indicate that boron pretreatment significantly protects rat bladder against cyclophosphamide-induced bladder damage due to its antiapoptotic and antioxidant properties.
dc.identifier.doi10.1007/s12011-019-01969-z
dc.identifier.endpage191
dc.identifier.issn0163-4984
dc.identifier.issn1559-0720
dc.identifier.issue1
dc.identifier.pmid31734911
dc.identifier.scopus2-s2.0-85080939425
dc.identifier.scopusqualityQ1
dc.identifier.startpage184
dc.identifier.urihttps://doi.org/10.1007/s12011-019-01969-z
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6187
dc.identifier.volume197
dc.identifier.wosWOS:000547390800020
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc
dc.relation.ispartofBiological Trace Element Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectCyclophosphamide
dc.subjectBladder
dc.subjectBoron
dc.subjectApoptosis
dc.subjectOxidative stress
dc.subjectThiol
dc.titleProtective Effects of Boron on Cyclophosphamide-Induced Bladder Damage and Oxidative Stress in Rats
dc.typeArticle

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