Newly developed peptide-ELISA successfully detected anti-IgG antibodies against Maedi-Visna virus in sheep

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dc.authoridKANDEMIR, CAGRI/0000-0001-7378-6962
dc.authoridPehlivan, Erkan/0000-0003-2505-1456
dc.contributor.authorKockaya, Ecem Su
dc.contributor.authorCan, Huseyin
dc.contributor.authorYaman, Yalcin
dc.contributor.authorKandemir, Cagri
dc.contributor.authorTaskin, Turgay
dc.contributor.authorKarakavuk, Muhammet
dc.contributor.authorDoskaya, Aysu Degirmenci
dc.date.accessioned2024-12-24T19:27:47Z
dc.date.available2024-12-24T19:27:47Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractMaedi Visna Virus (MVV) is a retrovirus that can infect sheep. There is still no effective therapy or vaccine against this virus and timely diagnosis is important to combat the complications of the disease. In this study, we aimed to develop an ELISA using peptides derived from gag protein as antigen. For this purpose, B cell epitopes of gag protein were predicted and a docking analysis with the B cell receptor was performed to select peptides to be used in ELISA. After three soluble epitopes with the highest antigenicity were produced as peptides, the immunogenicity of each peptide was determined by ELISA using sheep serum samples categorized as MVV positive (n=24) and negative (n=13). Subsequently, in house ELISA using above mentioned immunogenic peptides as antigen was used to investigate MVV seroprevalence in sheep (n=88). According to the results, among three peptides, two of them strongly reacted with MVV positive serum samples and the mean absorbance values detected among positive and negative serum samples were statistically significant, indicating that these peptides were immunogenic (P=0.016 and P=0.038). The third peptide also reacted with positive serum samples but the mean absorbance value was not statistically significant and this peptide was considered non-immunogenic (P=0.175). The immunogenic two peptides showed the same high sensitivity and specificity values of 91.60 and 92.80 according to the commercial kit. Moreover, MVV seroprevalence detected by peptide-ELISAs using CKQGSKE and CRPQGKAGHKG peptides as antigen was 3.40 % and 4.5 %, respectively. As a result, it was shown that these peptides can be successfully used for serological diagnosis of MVV.
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit [TGA-2022-23604]
dc.description.sponsorshipThis study was supported by a project given by the Ege University Scientific Research Projects Coordination Unit (Project number: TGA-2022-23604) to C.UE.
dc.identifier.doi10.1016/j.vetimm.2024.110806
dc.identifier.issn0165-2427
dc.identifier.issn1873-2534
dc.identifier.pmid39002364
dc.identifier.scopus2-s2.0-85198045116
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.vetimm.2024.110806
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6800
dc.identifier.volume274
dc.identifier.wosWOS:001270376000001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofVeterinary Immunology and Immunopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectMVV
dc.subjectPeptide-ELISA
dc.subjectSheep
dc.subjectSeroprevalence
dc.subjectT & uuml;rkiye
dc.titleNewly developed peptide-ELISA successfully detected anti-IgG antibodies against Maedi-Visna virus in sheep
dc.typeArticle

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