A pilot study on the epidemiology, diagnosis and characterization of Echinococcus granulosus sensu lato in sheep, goats and dogs in Siirt province of Türkiye revealed remarkable adaptation of Echinococcus canadensis (G6/ G7) in goats

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dc.authoridTekin, Afra Sena/0009-0009-5637-5075
dc.contributor.authorSelcuk, Muhammed Ahmed
dc.contributor.authorCelik, Burcak Aslan
dc.contributor.authorCelik, Figen
dc.contributor.authorCelik, Ozgur Yasar
dc.contributor.authorErcan, Kerem
dc.contributor.authorUslug, Muhammet
dc.contributor.authorTekin, Afra Sena
dc.date.accessioned2024-12-24T19:27:48Z
dc.date.available2024-12-24T19:27:48Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractCystic echinococcosis (CE), caused by Echinococcus granulosus sensu lato (s.l.), represents one of the most significant zoonotic diseases globally, affecting both humans and animals. The objective of this study was to ascertain the prevalence of E. granulosus sensu lato in sheep and goats in a pilot region with a one-year slaughterhouse follow-up period and to determine the genetic differences and haplotypes among sheep, goat, and dog isolates. To this end, the prevalence of CE cysts was determined by monitoring the slaughter of sheep and goats at least three days a week at a slaughterhouse in the Siirt province of T & uuml;rkiye during 2023. Additionally, faecal samples were collected from stray dogs and analysed using both flotation and molecular techniques. The presence of CE cysts was identified in 569 (11.12 %) of the 5119 sheep and 66 (2.31 %) of the 2860 goats after slaughtering. The highest positivity was observed in November (20.39 %), while the lowest was recorded in July (5.62 %). Of the sheep that detected positive, 25 (4.39 %) were less than one year old, while 544 (95.61 %) were older than one year. Of the infected sheep, 26 (4.57 %) were male and 543 (95.43 %) were female. 204 (35.85 %) sheep exhibited fluid-filled CE cysts, 338 (59.40 %) displayed calcification, and 27 (4.75 %) demonstrated the presence of newly developed cysts. The highest positivity was observed in December (5.83 %), while the lowest was recorded in May (0.62 %) in goats. Of the positive goats, two (3 %) were less than one year old, while the remaining 64 (97 %) were older than one year. Of the goats infected with CE cysts, 10 (15.15 %) were male and 56 (84.85 %) were female. Of the cysts, 56.1 % were fluid-filled, 42.4 % were calcified and 1.5 % were newly developed. Following DNA sequence analysis of CE cyst isolates obtained from the slaughterhouse, all 61 sheep sequences were identified as E. granulosus s.s. (G1/G3). Of the 13 goat isolates, seven were identified as E. granulosus s.s. (G1/G3), while the remaining six were classified as E. canadensis (G6/ G7). The centrifugal flotation method was employed to detect the presence of Isospora spp. oocysts in eight dogs, Toxocara canis and hookworm eggs in three dogs each, and Dipyllidium caninum eggs in one dog. A total of 54 dog faeces were examined. No Taeniid eggs were observed in any of the dogs. Following PCR analysis of the mt-CO1 gene region in the dog faecal samples, four samples were positive for a 875 bp band. Only one of these bands was suitable for sequence analysis, which confirmed it as E. granulosus s.s. (G1/G3).
dc.description.sponsorshipScientific Research Projects Unit of Siirt University [2023-I bull; HTVET-07]
dc.description.sponsorshipThis study was financially supported by a grant (2023-I center dot HTVET-07) from the Scientific Research Projects Unit of Siirt University.
dc.identifier.doi10.1016/j.vetpar.2024.110320
dc.identifier.issn0304-4017
dc.identifier.issn1873-2550
dc.identifier.pmid39383687
dc.identifier.scopus2-s2.0-85205936873
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.vetpar.2024.110320
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6802
dc.identifier.volume332
dc.identifier.wosWOS:001332630500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofVeterinary Parasitology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectEchinococcus granulosus
dc.subjectSheep
dc.subjectGoat
dc.subjectDog
dc.subjectPrevalance
dc.subjectGenotyping
dc.titleA pilot study on the epidemiology, diagnosis and characterization of Echinococcus granulosus sensu lato in sheep, goats and dogs in Siirt province of Türkiye revealed remarkable adaptation of Echinococcus canadensis (G6/ G7) in goats
dc.typeArticle

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