Investigation of the protective effect of chitosan against arsenic-induced nephrotoxicity and oxidative damage in rat kidney tissue

Basit Öğe Kaydı
dc.authoridBOLAT, ISMAIL/0000-0003-1398-7046
dc.contributor.authorIrak, K.
dc.contributor.authorCelik, O. Y.
dc.contributor.authorBolacali, M.
dc.contributor.authorTufan, T.
dc.contributor.authorOzcan, C.
dc.contributor.authorYildirim, S.
dc.contributor.authorBolat, I.
dc.date.accessioned2024-12-24T19:30:47Z
dc.date.available2024-12-24T19:30:47Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractArsenic is an important metalloid that can cause poisoning in humans and domestic animals. Exposure to arsenic causes cell damage, increasing the production of reactive oxygen species. Chitosan is a biopolymer obtained by deacetylation of chitin with antioxidant and metal ion chelating properties. In this study, the protective effect of chitosan on arsenic -induced nephrotoxicity and oxidative damage was investigated. 32 male Wistar-albino rats were divided into 4 groups of 8 rats each as control group (C), chitosan group (CS group), arsenic group (AS group), and arsenic+chitosan group (AS+CS group). The C group was given distilled water by oral gavage, the AS group was given 100 ppm/day Na-arsenite ad libitum with drinking water, the CS group was given 200 mg/kg/day chitosan dissolved in saline by oral gavage, the AS+CS group was given 100 ppm/day Na-arsenite ad libitum with drinking water and 200 mg/kg/day chitosan dissolved in saline by oral gavage for 30 days. At the end of the 30 -day experimental period, 90 mg/kg ketamine was administered intraperitoneally to all rats, and blood samples and kidney tissues were collected. Urea, uric acid, creatinine, P, Mg, K, Ca, Na, Cystatin C (CYS-C), Neutrophil Gelatinase Associated Lipocalin (NGAL) and Kidney Injury Molecule 1 (KIM -1) levels were measured in serum samples. Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT) and Superoxide dismutase (SOD) levels in the supernatant obtained from kidney tissue were analyzed by ELISA method. Compared with AS group, uric acid and creatinine levels of the AS+CS group were significantly decreased (p<0.001), urea, KIM -1, CYS-C, NGAL, and MDA levels were numerically decreased and CAT, GSH, and SOD levels were numerically increased (p>0.05). In conclusion, based on both biochemical and histopathological-immunohistochemical-immunofluorescence findings, it can be concluded that chitosan attenuates kidney injury and protects the kidney.
dc.description.sponsorshipSiirt University Scientific Research Projects Coordination Office [2021-SIdot;VET-06]
dc.description.sponsorshipThis research was supported by Siirt University Scientific Research Projects Coordination Office within the scope of project number 2021-S & Idot;UEVET-06.
dc.identifier.doi10.24425/pjvs.2024.149339
dc.identifier.endpage105
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue1
dc.identifier.pmid38511628
dc.identifier.scopus2-s2.0-85188499873
dc.identifier.scopusqualityQ3
dc.identifier.startpage95
dc.identifier.urihttps://doi.org/10.24425/pjvs.2024.149339
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7677
dc.identifier.volume27
dc.identifier.wosWOS:001189097600007
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPolska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn
dc.relation.ispartofPolish Journal of Veterinary Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectarsenic
dc.subjectchitosan
dc.subjectnephrotoxicity
dc.subjectoxidative stress
dc.subjectrat
dc.titleInvestigation of the protective effect of chitosan against arsenic-induced nephrotoxicity and oxidative damage in rat kidney tissue
dc.typeArticle

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu