High Level Production of Extracellular Protease from Bacillus licheniformis ATCC 12759 in Submerged Fermentation
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Date
2012
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Research Journal Biotechnology
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Production of extracellular protease by Bacillus licheniformis ATCC 12759 in submerged fermentation was the aim of this study. Various nutrients belonging to three categories, carbon, nitrogen and amino acid sources, were investigated in terms of their effect on the production of extracellular protease by Bacillus licheniformis ATCC 12759. Comparison with the control in media containing the all simple sugars resulted in a decrease in proteolytic activity, while there was significant increase in enzyme yield in the case of the supplementation complex carbon source such as wheat flour and rice flour. Urea, soy flour and sodium nitrate are the best organic and inorganic sources respectively. Among the amino acid sources tested, L-phenylalanine, L-cysteine, Glysine, L-valine, L-tyrosine, L-isoleusine, L-tryptophane and Glutamic acid favored the production respectively. FeSO4, ZnSO4 and CuSO4 inhibited protease production. Maximum protease production (10738.2 +/- 44.2 U/mg) was obtained in a medium containing 0.1% MgSO4 in 24 h 37 degrees C.
Description
Keywords
Bacillus licheniformis ATCC 12759, protease, submerged fermentation, enzyme production
Journal or Series
Research Journal of Biotechnology
WoS Q Value
Q4
Scopus Q Value
Q4
Volume
7
Issue
3
