Enzymeless copper microspheres@carbon sensor design for sensitive and selective acetylcholine screening in human serum

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dc.authoridShenashen, Mohamed/0000-0003-1592-5877
dc.authoridEl-Safty, Sherif/0000-0001-5992-9744
dc.authoridSelim, Mahmoud/0000-0002-9727-4520
dc.authoridEmran, Mohammed Y./0000-0002-0605-7118
dc.contributor.authorEmran, Mohammed Y.
dc.contributor.authorShenashen, Mohamed A.
dc.contributor.authorEl Sabagh, Ayman
dc.contributor.authorSelim, Mahmoud M.
dc.contributor.authorEl-Safty, Sherif A.
dc.date.accessioned2024-12-24T19:26:59Z
dc.date.available2024-12-24T19:26:59Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractFollow up of neuronal disorders, such as Alzheimer's and Parkinson's diseases using simple, sensitive, and selective assays is urgently needed in clinical and research investigation. Here, we designed a sensitive and selective enzymeless electrochemical acetylcholine sensor that can be used in human fluid samples. The designed electrode consisted of a micro spherical construction of Cu-metal decorated by a thin layer of carbon (CuMS@C). A simple and one-pot synthesis approach was used for Cu-metal controller formation with a spherical like structures. The spherical like structure was formed with rough outer surface texture, circular build up, homogeneous formation, micrometric spheres size (0.5 -1 mu m), and internal hollow structure. The formation of a thin layer of carbon materials on the surface of CuMS sustained the catalytic activity of Cu atoms and enriched negatively charge of the surface. CuMS@C acted as a highly active mediator surface that consisted of Cu metal as a highly active catalyst and carbons as protecting, charge transport, and attractive surface. Therefore, the CuMS@C surface morphology and composition played a key role in various aspects such as facilitated ACh diffusion/loading, increased the interface surface area, and enhanced the catalytic activity. The CuMS@C acted as an electroactive catalyst for ACh electrooxidation and current production, due to the losing of two electrons. The fabricated CuMS@C could be a highly sensitive and selective enzymeless sensor for detecting ACh with a detection limit of 0.1 mu M and a wide linear range of 0.01 - 0.8 mM. The designed ACh sensor assay based on CuMS@C exhibited fast sensing property as well as sensitivity, selectivity, stability, and reusability for detecting ACh in human serum samples. This work presents the design of a highly active electrode surface for direct detection of ACh and further clinical investigation of ACh levels.
dc.description.sponsorshipJapan Society for the Promotion of Science (JSPS) [P19067]
dc.description.sponsorshipThis work was supported by the Japan Society for the Promotion of Science (JSPS), grant No. P19067.
dc.identifier.doi10.1016/j.colsurfb.2021.112228
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid34839049
dc.identifier.scopus2-s2.0-85119915006
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2021.112228
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6440
dc.identifier.volume210
dc.identifier.wosWOS:000734889800005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofColloids and Surfaces B-Biointerfaces
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectNonenzymatic sensors
dc.subjectCu microspheres
dc.subjectHybrid materials
dc.subjectAcetylcholine
dc.subjectHuman fluids
dc.subjectNeuronal diseases
dc.titleEnzymeless copper microspheres@carbon sensor design for sensitive and selective acetylcholine screening in human serum
dc.typeArticle

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