Expression patterns and distribution of aquaporin water channels in cervix as a possible mechanism for cervical patency in bitches affected by pyometra

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dc.authoridALTINBAS, Yunus Furkan/0000-0002-3934-8018
dc.authoridOzoner, Ozgur/0000-0001-7354-0655
dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.authoridOzkan, Huseyin/0000-0001-5753-8985
dc.contributor.authorYazlik, Murat Onur
dc.contributor.authorOzkan, Hueseyin
dc.contributor.authorVural, Sevil Atalay
dc.contributor.authorKaya, Ufuk
dc.contributor.authorOzoner, Ozgur
dc.contributor.authorMutluer, Ipek
dc.contributor.authorAltinbas, Yunus Furkan
dc.date.accessioned2024-12-24T19:27:46Z
dc.date.available2024-12-24T19:27:46Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractPyometra is a life-threatening disease, the severity of which depends on cervical patency status. This study investigated cervical inflammation status as well as the expression patterns and localization of aquaporin (AQP1, AQP2, AQP3, AQP5, and AQP9), and hormone receptors in cervical tissue that influences canine pyometra. Of the 36 animals enrolled in the study, 24 were diagnosed with pyometra and separated into two groups: open cervix pyometra and close cervix pyometra, while 12 healthy animals presented for elective ovariohysterectomies were allocated into the control group. Surgical treatment was performed for treatment of pyometra. After each operation, cervix samples were collected and analyzed for AQP and hormone receptor expression patterns determined by qPCR and protein expression by means of immunohistochemistry. Blood samples were also collected to determine serum progesterone concentrations. AQP9 expression was downregulated approximately 3-fold while and PGR expression was downregulated more than 2 fold in both pyometra groups compared to the control group. AQP3 and AQP5 gene expression levels were upregulated more than 3 fold in the open-cervix pyometra group than the closed-cervix pyometra group (P < 0.05). This is the first study to describe the expression patterns and immunolocalization of AQPs in canine cervical tissue based on pyometra patency status and to report AQP3 and AQP5 expression in cervical tissue linked to cervical patency.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK), Turkiye [120O543]
dc.description.sponsorshipThis study was conducted as a project supported by the The Scientific and Technological Research Council of Turkiye (TUBITAK) (Project number: 120O543) , Turkiye.
dc.identifier.doi10.1016/j.theriogenology.2024.07.022
dc.identifier.endpage143
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.pmid39067211
dc.identifier.scopus2-s2.0-85199449141
dc.identifier.scopusqualityQ1
dc.identifier.startpage138
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2024.07.022
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6792
dc.identifier.volume227
dc.identifier.wosWOS:001284212300001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofTheriogenology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectAquaporin
dc.subjectCanine
dc.subjectCervix
dc.subjectPatency
dc.titleExpression patterns and distribution of aquaporin water channels in cervix as a possible mechanism for cervical patency in bitches affected by pyometra
dc.typeArticle

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