Investigation of the Effect of Some Optically Active Imine Compounds on the Enzyme Activities of hCA-I and hCA-II under In Vitro Conditions: An Experimental and Theoretical Study

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dc.authoridBaykara, Haci/0000-0002-8319-0836
dc.contributor.authorTektas, Osman
dc.contributor.authorAkkemik, Ebru
dc.contributor.authorBaykara, Haci
dc.date.accessioned2024-12-24T19:24:11Z
dc.date.available2024-12-24T19:24:11Z
dc.date.issued2016
dc.departmentSiirt Üniversitesi
dc.description.abstractInhibitors of carbonic anhydrase (hCA; EC 4.2.1.1) are used as medicines for many diseases. Therefore, they are very important. In this study, a known series of Schiff bases were synthesized and their effects on the activities of hCA-I and hCA-II, which are cytosolic isoenzymes of carbonic anhydrase, were investigated under in vitro conditions. The synthesized compounds (H1, H2, H3, and H4) were found to cause inhibition on enzyme activities of hCA-1 and hCA-II. IC50 values of H1, H2, H3, and H4 compounds were 140, 88, 201, and 271 M for hCA-I enzyme activity and 134, 251, 79, and 604 M for hCA-II enzyme activity, respectively. The synthesized Schiff bases were characterized by several methods, including H-1 NMR, FT-IR, elemental analysis, and polarimetric measurements. Correlation coefficient square values (R-2) of comparison of the theoretical and experimental H-1 NMR shifts for H1, H2, H3, and H4 compounds were found as 0.9781, 0.9814, 0.9758, and 0.8635, respectively.
dc.description.sponsorshipScientific Research Projects Unit of Siirt University [2013-SIUFED-F6]
dc.description.sponsorshipContract Grant Sponsor: Scientific Research Projects Unit of Siirt University. Contract Grant Number: 2013-SIUFED-F6.
dc.identifier.doi10.1002/jbt.21788
dc.identifier.endpage286
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue6
dc.identifier.pmid26762592
dc.identifier.scopus2-s2.0-84973439841
dc.identifier.scopusqualityQ2
dc.identifier.startpage277
dc.identifier.urihttps://doi.org/10.1002/jbt.21788
dc.identifier.urihttps://hdl.handle.net/20.500.12604/5882
dc.identifier.volume30
dc.identifier.wosWOS:000378506400002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Biochemical and Molecular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectCarbonic Anhydrase
dc.subjectDFT
dc.subjectChirality
dc.subjectInhibition
dc.subjectTheoretical NMR
dc.titleInvestigation of the Effect of Some Optically Active Imine Compounds on the Enzyme Activities of hCA-I and hCA-II under In Vitro Conditions: An Experimental and Theoretical Study
dc.typeArticle

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