CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges

Basit Öğe Kaydı
dc.authoridWani, Shabir/0000-0002-7456-4090
dc.authoridEL Sabagh, Ayman/0000-0002-4241-192X
dc.authoridVachova, Pavla/0000-0002-6510-7218
dc.authoridCIG, Fatih/0000-0002-4042-0566
dc.authoridTyagi, Anshika/0000-0001-9892-0566
dc.authoridHejnak, Vaclav/0000-0003-0893-1486
dc.authoridDar, Tanvir/0000-0003-2076-4449
dc.authoridDAR, AEJAZ AHMAD/0000-0001-9418-6990
dc.authoridZaid, Abbu/0000-0002-2090-0212
dc.authoridRahman, Muhammad Habib ur/0000-0002-2823-9959
dc.authoridSkalicky, Milan/0000-0002-4114-6909
dc.authoridBrestic, Marian/0000-0003-3470-6100
dc.authoridShah, Muntazir Mushtaq/0000-0002-2725-5455
dc.authoridRai, Gyanendra Kumar/0000-0002-2638-0043
dc.authoridBasu, Umer/0000-0003-3473-9025
dc.contributor.authorMushtaq, Muntazir
dc.contributor.authorAhmad Dar, Aejaz
dc.contributor.authorSkalicky, Milan
dc.contributor.authorTyagi, Anshika
dc.contributor.authorBhagat, Nancy
dc.contributor.authorBasu, Umer
dc.contributor.authorBhat, Basharat Ahmad
dc.date.accessioned2024-12-24T19:33:36Z
dc.date.available2024-12-24T19:33:36Z
dc.date.issued2021
dc.departmentSiirt Üniversitesi
dc.description.abstractGenome-editing (GE) is having a tremendous influence around the globe in the life science community. Among its versatile uses, the desired modifications of genes, and more importantly the transgene (DNA)-free approach to develop genetically modified organism (GMO), are of special interest. The recent and rapid developments in genome-editing technology have given rise to hopes to achieve global food security in a sustainable manner. We here discuss recent developments in CRISPR-based genome-editing tools for crop improvement concerning adaptation, opportunities, and challenges. Some of the notable advances highlighted here include the development of transgene (DNA)-free genome plants, the availability of compatible nucleases, and the development of safe and effective CRISPR delivery vehicles for plant genome editing, multi-gene targeting and complex genome editing, base editing and prime editing to achieve more complex genetic engineering. Additionally, new avenues that facilitate fine-tuning plant gene regulation have also been addressed. In spite of the tremendous potential of CRISPR and other gene editing tools, major challenges remain. Some of the challenges are related to the practical advances required for the efficient delivery of CRISPR reagents and for precision genome editing, while others come from government policies and public acceptance. This review will therefore be helpful to gain insights into technological advances, its applications, and future challenges for crop improvement.
dc.description.sponsorshipMinistry of Education, Youth and Sports of the Czech Republic
dc.description.sponsorshipThis study was supported by an S-grant from the Ministry of Education, Youth and Sports of the Czech Republic.
dc.identifier.doi10.3390/genes12060797
dc.identifier.issn2073-4425
dc.identifier.issue6
dc.identifier.pmid34073848
dc.identifier.scopus2-s2.0-85107429839
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3390/genes12060797
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8218
dc.identifier.volume12
dc.identifier.wosWOS:000667498800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofGenes
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectgenome editing
dc.subjectCRISPR
dc.subjectbase editing
dc.subjectprime editing
dc.subjectDNA-free genome editing
dc.subjectcrop improvement
dc.titleCRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges
dc.typeReview Article

Dosyalar

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu