Metal chelate based site recognition of ceruloplasmin using molecularly imprinted polymer/cryogel system
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Group
Erişim Hakkı
info:eu-repo/semantics/restrictedAccess
Özet
In the present study, N-Acetylneuraminic acid (NANA)-imprinted poly(2-hydroxyethyl methacrylate-
N-methacryloyl-(L)-histidin-Cu(II)) p(HEMA-(MAH)2-Cu(II)) has been synthesized by radical
polymerization for site recognition of ceruloplasmin. Prepared imprinted cryogel has been characterized
by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy
(FTIR). When the binding capacity of NANA-imprinted cryogel (MIP) was compared with nonimprinted
cryogel (NIP), it was found that MIP has higher adsorption capacity. The maximum
amount of binding NANA has found as 83.20 mgg−1 at pH 7.0 with flow rate of 1 mLmin−1 at 25°C.
Selectivity experiments for MIP and NIP cryogel have been carried out via the NANA/D-mannose
and ceruloplasmin/immunoglobulin G (IgG) pair separately and the relative selectivity coefficient
(k’) has found as 77.46 and 17.97, respectively. Applicable of the MIP in human serum has been
studied and resulted successfully.
Açıklama
Anahtar Kelimeler
Ceruloplasmin, NANA-imprinted cryogel, metal-chelate complex, p-hema-mah, selective separation
Kaynak
Separation Science and Technology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
55
Sayı
2
Künye
Dolak, I., Canpolat, G., Ersöz, A., & Say, R. (2019). Metal chelate based site recognition of ceruloplasmin using molecularly imprinted polymer/cryogel system. Separation Science and Technology, 1-10.
