Decreased Na+/K+ pump activity in the erythrocyte membrane due to malondialdehyde in rheumatoid arthritis: an in vivo and in silico study

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dc.authoridGUR, Bahri/0000-0003-0579-6354
dc.authoridCENGIZ, Mustafa/0000-0002-6925-8371
dc.contributor.authorOgul, Yasemin
dc.contributor.authorGur, Fatma
dc.contributor.authorGur, Bahri
dc.contributor.authorCengiz, Mustafa
dc.contributor.authorSari, Refik Ali
dc.contributor.authorKiziltunc, Ahmet
dc.date.accessioned2024-12-24T19:29:39Z
dc.date.available2024-12-24T19:29:39Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractApart from demonstrating the interaction behavior of malondialdehyde (MDA) with Na+/K+-ATPase using in silico, the current study aims to investigate the effect of rheumatoid arthritis-related oxidative stress on Na+/K+-ATPase activity that is present in the erythrocyte cell membrane, which is rich in proteins vulnerable to damage from MDA and other free radicals. The target population of this study consists of 28 rheumatoid arthritis patients and 20 healthy volunteers whose MDA levels and Na+/K+-ATPase activity were determined. It was shown that MDA levels of rheumatoid arthritis patients increased (p < 0.001) and their Na+/K+-ATPase activity noticeably decreased when compared to those of healthy individuals. Also, according to this in silico modeling, MDA decreased Na+/K+-ATPase activity in line with the correlation analyses. Consequently, while elevated levels of MDA in the rheumatoid arthritis group were suggestive of oxidative stress, a decreased Na+/K+-ATPase-activity led us to speculate that the cellular membrane had sustained injury. Therefore, our results could be useful in explaining how MDA affects Na+/K+-ATPase activity in the interior of a specific molecular pathway.
dc.description.sponsorshipAtaturk University
dc.description.sponsorshipThe author thanks Ataturk University for its generous support. The numerical computations in this study were carried out at the TUBITAK ULAKBIM High Performance and Grid Computing (TR-Grid) Centre. The authors genuinely thank Dr. Zeynep Silan Turhan, who works at Igdir University, for her assistance in molecular dynamics and simulation studies.
dc.identifier.doi10.1139/cjpp-2022-0171
dc.identifier.issn0008-4212
dc.identifier.issn1205-7541
dc.identifier.pmid36148907
dc.identifier.scopus2-s2.0-85139483989
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1139/cjpp-2022-0171
dc.identifier.urihttps://hdl.handle.net/20.500.12604/7187
dc.identifier.wosWOS:000861089600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCanadian Science Publishing
dc.relation.ispartofCanadian Journal of Physiology and Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectNa+/K+-ATPase activity
dc.subjectrheumatoid arthritis
dc.subjectlipid peroxidation
dc.subjectnegative binding energy
dc.subjectmolecular docking
dc.titleDecreased Na+/K+ pump activity in the erythrocyte membrane due to malondialdehyde in rheumatoid arthritis: an in vivo and in silico study
dc.typeArticle

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