Acinetobacter mesopotamicussp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey

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dc.authoridAcer, Omer/0000-0002-5314-0475
dc.authoridFinore, Ilaria/0000-0003-3606-5140
dc.authoridBuono, Lorena/0000-0002-5457-4515
dc.contributor.authorAcer, Omer
dc.contributor.authorGuven, Kemal
dc.contributor.authorPoli, Annarita
dc.contributor.authorDi Donato, Paola
dc.contributor.authorLeone, Luigi
dc.contributor.authorBuono, Lorena
dc.contributor.authorGuven, Reyhan Gul
dc.date.accessioned2024-12-24T19:24:24Z
dc.date.available2024-12-24T19:24:24Z
dc.date.issued2020
dc.departmentSiirt Üniversitesi
dc.description.abstractA new petroleum-degrading bacterium, designated strain GC2(T), was isolated from Bozkus 1 petroleum station in Diyarbakir, located in the southeast of Turkey. Cells were Gram-negative staining, aerobic, coccoid-rods, non-motile, non-spore-forming. The bacterium was found to degrade 100% ofn-alkanes ranging from C11 to C34 presented in the 1% crude oil after incubation of 7 days. The membrane phospholipids were 1,2 diacylglycero-3-phosphorylethanolamine (PEA), phosphatidylglycerol (PG), dipalmitoyl-sn-glycerol 1- phosphocholine (PC1), 1,2 dipalmitoyl-sn-glycero-3-phosphocholine monohydrate (PC3), cardiolipin also called diphosphatidylglycerol (CL) and l-alpha- phosphatidic acid, dipalmitoyl (AP); predominant respiratory ubiquinone was Q-8 and C16:0, C18:1 omega 9c and C16:1 were the major cellular fatty acids. The 16S rRNA sequence analysis revealed that the strain GC2(T)was a member of genusAcinetobacterand was most closely related toAcinetobacter lwoffiiDSM 2403( T)(99.79%),Acinetobacter pseudolwoffiiANC 5318( T)(98.83%) andAcinetobacter harbinensisHITLi 7( T)(98.14%). TherpoBandgyrBgene sequence analysis confirmed that the strain GC2(T)was a member of genusAcinetobacterand that the closest relative wasAcinetobacter lwoffiiDSM 2403( T)(99.08% and 100% similarity, respectively). DNA-DNA hybridization values between GC2(T)and its closest relatives ranged from 65.6% (withA. lwoffii) to 5.1% (withA. venetianus). The whole genome sequence of strain GC2(T)was obtained. The DNA G + C content of this strain was determined to be 42.9 mol %. ANI indexes, in silico estimations of DDH values and wet lab DDH values demonstrated that strain GC2(T)represents an independent genomospecies. On the basis of phenotypic characteristics, chemotaxonomic, phylogenetic data and DNA-DNA hybridization and whole genome analysis, we propose to assign strain GC2(T)as a new species of the genusAcinetobacter, for which the nameAcinetobacter mesopotamicussp. nov. is proposed. The type strain of this species is GC2(T)(DSM 26953 T = JCM 31073 T). The whole genome of strain GC2(T)has been deposited at DDBJ/ENA/GenBank under the accession JAALFF010000000.
dc.description.sponsorshipScientific Research Commission of Dicle University-Diyarbakir [DUAPK-12-FF-149]; [BioPoliS PON03PE_00107_1]
dc.description.sponsorshipThe paper was partially supported by the Scientific Research Commission of Dicle University-Diyarbakir (Projects DUAPK-12-FF-149) and by project BioPoliS PON03PE_00107_1 'Development of green technologies for production of BIOchemicals and their use in preparation and industrial application of POLImeric materials from agricultural biomasses cultivated in a sustainable way in Campania region'.
dc.identifier.doi10.1007/s00284-020-02134-9
dc.identifier.endpage3200
dc.identifier.issn0343-8651
dc.identifier.issn1432-0991
dc.identifier.issue10
dc.identifier.pmid32725341
dc.identifier.scopus2-s2.0-85088802755
dc.identifier.scopusqualityQ3
dc.identifier.startpage3192
dc.identifier.urihttps://doi.org/10.1007/s00284-020-02134-9
dc.identifier.urihttps://hdl.handle.net/20.500.12604/5973
dc.identifier.volume77
dc.identifier.wosWOS:000553299600002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofCurrent Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.titleAcinetobacter mesopotamicussp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey
dc.typeArticle

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