Biogenic Synthesized Bare and Boron-Doped Copper Oxide Nanoparticles from Thymbra spicat ssp. spicata: In Silico and In Vitro Studies

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dc.authoridBAYTAR, ORHAN/0000-0002-2915-202X
dc.authoridCENGIZ, Mustafa/0000-0002-6925-8371
dc.authoridGUR, Bahri/0000-0003-0579-6354
dc.contributor.authorCengiz, Mustafa
dc.contributor.authorBaytar, Orhan
dc.contributor.authorSahin, Omer
dc.contributor.authorKutlu, Hatice Mehtap
dc.contributor.authorAyhanci, Adnan
dc.contributor.authorSezer, Canan Vejselova
dc.contributor.authorGur, Bahri
dc.date.accessioned2024-12-24T19:24:40Z
dc.date.available2024-12-24T19:24:40Z
dc.date.issued2024
dc.departmentSiirt Üniversitesi
dc.description.abstractThe biosynthesis technique and biogenic copper oxide nanoparticles (CuONPs) are commonly used in a variety of applications including medicine. Bare (CuONPs) and boron-doped copper oxide nanoparticles (B/CuONPs) were produced via the green synthesis method using Thymbra spicat ssp. spicata due to their nontoxic, coast effective and facile properties. The nanoparticles were characterized by using X-ray diffraction, fourier transform infrared, UV-visible spectroscopy, transmission electron microscopy, and Scanning electron microscopy with Energy Dispersive X-ray spectroscopy analysis. The produced nanoparticles performed antibacterial activity against human pathogenic organisms of both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria by using the microdilution technique. B/CuONPs showed high activity on Gram-positive bacteria, while CuONPs showed high activity on Gram-negative bacteria. The cytotoxic effect synthesized CuONPs and B/CuONPs were evaluated against human hepatocarcinoma (HepG2) cells by using MTT, Annexin-V, Caspase-3/7, and confocal microscopic evaluations. Moreover, the in-silico results have shown for the first time that the active role in the Caspase-3/7 step of the triggered apoptosis pathway is due to the activity of Caspase-7. The results indicated that the biogenic CuONPs and B/CuONPs exerted potential anti-cancer and anti-bacterial activity on HepG2 and S. aureus and E. coli that imply to remarkable biological activity. The green synthesized nanoparticles have clearly proposed promising biogenic nanomaterials for biomedical treatments.
dc.identifier.doi10.1007/s10876-023-02481-0
dc.identifier.endpage284
dc.identifier.issn1040-7278
dc.identifier.issn1572-8862
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85165972452
dc.identifier.scopusqualityQ1
dc.identifier.startpage265
dc.identifier.urihttps://doi.org/10.1007/s10876-023-02481-0
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6090
dc.identifier.volume35
dc.identifier.wosWOS:001034206500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer/Plenum Publishers
dc.relation.ispartofJournal of Cluster Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectGreen synthesis
dc.subjectCaspase-3
dc.subject7 activation
dc.subjectIn silico modeling
dc.subjectCopper oxide nanoparticle
dc.subjectHepatocellular carcinoma
dc.titleBiogenic Synthesized Bare and Boron-Doped Copper Oxide Nanoparticles from Thymbra spicat ssp. spicata: In Silico and In Vitro Studies
dc.typeArticle

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