Biogenic Synthesized Bare and Boron-Doped Copper Oxide Nanoparticles from Thymbra spicat ssp. spicata: In Silico and In Vitro Studies
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer/Plenum Publishers
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The biosynthesis technique and biogenic copper oxide nanoparticles (CuONPs) are commonly used in a variety of applications including medicine. Bare (CuONPs) and boron-doped copper oxide nanoparticles (B/CuONPs) were produced via the green synthesis method using Thymbra spicat ssp. spicata due to their nontoxic, coast effective and facile properties. The nanoparticles were characterized by using X-ray diffraction, fourier transform infrared, UV-visible spectroscopy, transmission electron microscopy, and Scanning electron microscopy with Energy Dispersive X-ray spectroscopy analysis. The produced nanoparticles performed antibacterial activity against human pathogenic organisms of both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria by using the microdilution technique. B/CuONPs showed high activity on Gram-positive bacteria, while CuONPs showed high activity on Gram-negative bacteria. The cytotoxic effect synthesized CuONPs and B/CuONPs were evaluated against human hepatocarcinoma (HepG2) cells by using MTT, Annexin-V, Caspase-3/7, and confocal microscopic evaluations. Moreover, the in-silico results have shown for the first time that the active role in the Caspase-3/7 step of the triggered apoptosis pathway is due to the activity of Caspase-7. The results indicated that the biogenic CuONPs and B/CuONPs exerted potential anti-cancer and anti-bacterial activity on HepG2 and S. aureus and E. coli that imply to remarkable biological activity. The green synthesized nanoparticles have clearly proposed promising biogenic nanomaterials for biomedical treatments.
Açıklama
Anahtar Kelimeler
Green synthesis, Caspase-3, 7 activation, In silico modeling, Copper oxide nanoparticle, Hepatocellular carcinoma
Kaynak
Journal of Cluster Science
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
35
Sayı
1
