In silico discovery of epitopes of gag and env proteins for the development of a multi-epitope vaccine candidate against Maedi Visna Virus using reverse vaccinology approach

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dc.contributor.authorKockaya, Ecem Su
dc.contributor.authorCan, Hueseyin
dc.contributor.authorYaman, Yalcin
dc.contributor.authorUen, Cemal
dc.date.accessioned2024-12-24T19:25:21Z
dc.date.available2024-12-24T19:25:21Z
dc.date.issued2023
dc.departmentSiirt Üniversitesi
dc.description.abstractMaedi Visna Virus (MVV) causes a chronic viral disease in sheep. Since there is no specific therapeutic drug that targets MVV, development of a vaccine against the MVV is inevitable. This study aimed to analyze the gag and env proteins as vaccine candidate proteins and to identify epitopes in these proteins. In addition, it was aimed to construct a multi-epitope vaccine candidate. According to the obtained results, the gag protein was detected to be more conserved and had a higher antigenicity value. Also, the number of alpha helix in the secondary structure was higher and transmembrane helices were not detected. Although many B cell and MHC-I/II epitopes were predicted, only 19 of them were detected to have the properties of antigenic, non-allergenic, non-toxic, soluble, and non-hemolytic. Of these epitopes, five were remarkable due to having the highest antigenicity value. However, the final multi-epitope vaccine was constructed with 19 epitopes. A strong affinity was shown between the final multi-epitope vaccine and TLR-2/4. In conclusion, the gag protein was a better antigen. However, both proteins had epitopes with high antigenicity value. Also, the final multi-epitope vaccine construct had a potential to be used as a peptide vaccine due to its immuno-informatics results.
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit [TGA-2022-23604]
dc.description.sponsorshipThis study was supported by a project given by the Ege University Scientific Research Projects Coordination Unit (Project number:TGA-2022-23604) to C.U.
dc.identifier.doi10.1016/j.biologicals.2023.101715
dc.identifier.issn1045-1056
dc.identifier.issn1095-8320
dc.identifier.pmid37793308
dc.identifier.scopus2-s2.0-85173156403
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.biologicals.2023.101715
dc.identifier.urihttps://hdl.handle.net/20.500.12604/6376
dc.identifier.volume84
dc.identifier.wosWOS:001149445300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.relation.ispartofBiologicals
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectMVV
dc.subjectB cell epitopes
dc.subjectMHC-I/II epitopes
dc.subjectImmuno-informatics
dc.subjectPeptide-based vaccine
dc.titleIn silico discovery of epitopes of gag and env proteins for the development of a multi-epitope vaccine candidate against Maedi Visna Virus using reverse vaccinology approach
dc.typeArticle

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