Purification and Characterization of Carbonic Anhydrase from A. gri Balik Lake Trout Gill (Salmo trutta labrax) and Effects of Sulfonamides on Enzyme Activity

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dc.authoridSenturk, Murat/0000-0001-5968-7511
dc.authoridKUZU, Muslum/0000-0002-1375-7673
dc.authoridYERLIKAYA, Emrah/0000-0003-4050-0790
dc.contributor.authorDemirdag, Ramazan
dc.contributor.authorComakli, Veysal
dc.contributor.authorKuzu, Muslum
dc.contributor.authorYerlikaya, Emrah
dc.contributor.authorSenturk, Murat
dc.date.accessioned2024-12-24T19:24:10Z
dc.date.available2024-12-24T19:24:10Z
dc.date.issued2015
dc.departmentSiirt Üniversitesi
dc.description.abstractCarbonic anhydrase (CA) was purified from Ar Balk Lake trout gill (fCA) by affinity chromatography on a sepharose 4B-tyrosine-sulfanilamide column. The fCA enzyme was purified with about a 303.9 purification factor, a specific activity 4130.4 EU (mg-protein)(-1), and a yield of 79.3 by using sepharose-4B-l tyrosine-sulfanilamide affinity gel chromatography. The molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was found to be about 29.9 kDa. The kinetic parameters, K-M and V-max were determined for the 4-nitrophenyl acetate hydrolysis reaction. Some sulfonamides were tested as inhibitors against the purified CA enzymes. The K-i constants for mafenide (1), p-toluenesulfonamide (2), 2-bromo-benzene sulfonamide (3), 4-chlorobenzene sulfonamide (4), 4-amino-6-chloro-1-3 benzenedisulfonamide (5), sulfamethazine (6), sulfaguanidine (7), sulfadiazine (8), and acetozazolamide (9) were in the range of 7.5-108.75 M. (C) 2014 Wiley Periodicals, Inc.
dc.description.sponsorshipAgri Ibrahim Cecen University Scientific Research Projects Unit (BAP) [BAP 2012/MYO-02]
dc.description.sponsorshipContract Grant Sponsor: Agri Ibrahim Cecen University Scientific Research Projects Unit (BAP). Contract Grant Number: BAP 2012/MYO-02.
dc.identifier.doi10.1002/jbt.21675
dc.identifier.endpage128
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue3
dc.identifier.pmid25345690
dc.identifier.scopus2-s2.0-84923929942
dc.identifier.scopusqualityQ2
dc.identifier.startpage123
dc.identifier.urihttps://doi.org/10.1002/jbt.21675
dc.identifier.urihttps://hdl.handle.net/20.500.12604/5881
dc.identifier.volume29
dc.identifier.wosWOS:000350662600004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Biochemical and Molecular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectAgri Balik Lake Trout
dc.subjectCarbonic Anhydrase
dc.subjectSulfonamides
dc.subjectInhibition
dc.subjectPurification
dc.subjectCharacterization
dc.titlePurification and Characterization of Carbonic Anhydrase from A. gri Balik Lake Trout Gill (Salmo trutta labrax) and Effects of Sulfonamides on Enzyme Activity
dc.typeArticle

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