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Öğe A novel lanthanide-chelate based molecularly imprinted cryogel for purification of hemoglobin from blood serum: An alternative method for thalassemia diagnosis(Elsevier Sci Ltd, 2020) Dolak, Ibrahim; Canpolat, Gurbet; Onat, Ruken; Kecili, Rustem; Baysal, Zubeyde; Ziyadanogullari, Berrin; Ersoz, ArzuPurification and analyzing of proteins is an essential means for understanding their function and diseases associated with their lack or defect. In this research, a new lanthanide-chelate based molecularly imprinted polymer (MIP) was synthesized for selective separation of Hemoglobin (Hb) from human serum in the presence of various interference molecules. The Hb-imprinted polymer was prepared by using complex functional monomer N-methacryloylamido antipyrine (MAAP)-Ce(III) and 2-Hydroxyethyl methacrylate (HEMA) in accordance of cryopolymerization techniques. The nonimprinted cryogel (NIP) was also prepared at same polymerization conditions in the absence of template Hb molecule. The effects of pH, initial Hb concentration, flow rate, temperature and ionic strength on the binding capacity of both imprinted and nonimprinted cryogels was investigated. The maximum binding capacity for the MIP column was found to be as 79.41 mg g(-1) dry cryogel, that is four times higher than the NIP column under the optimum conditions (pH 5.0, flow rate: 1.0 mL min(-1), T: 25 degrees C). Moreover, selectivity experiments were performed by using two interference proteins as myoglobin (Mb) and cytochrome c (Cyt-c) and the relative selectivity coefficients (k') for Hb/Mb and Hb/Cyt-c pairs were determined as 36.59 and 37.22, respectively.Öğe The antioxidant capacity, total phenolic content and phenolic compounds of Plantago coronopus L. subsp. coronopus in naturally distributed in Akdoğmuş-Siirt(IEEE, 2018) Canpolat, Gurbet; Teğin, İbrahim; Fidan, MehmetName probably for Plantago coronopus L.; in reference to the shape of basal leaves [1]. Plantago coronopus, commonly known as buckshorn banana (“diabelha” in Portugal), is a medical halophyte belonging to the Plantago L., the largest of the Plantaginaceae family [2]. Plantago will be those the vast majority paramount variety of the Plantaginaceae crew, What's more, will be utilized within customary prescription around the reality for separate purposes. Plantago coronopus L., Plantago major L., Plantago media L. also Plantago lanceolata L. would a large portion regularly utilized species of Plantago for a customary solution to turkey [3]. There would 28 taxa of the class Plantago did turkey and two of them are endemic [4]. In this study, extracts of Plantago coronopus L. subsp. the coronopus taxon was prepared with methanol (%80) solvent. Total phenolic contents were assessed by Folin Ciocalteau's method and were found as 32.66 mg gallic acid /mL extract. The antioxidant activity was determined by using DPPH methods as 87.26%. And also, the chemical composition of the phenolic compounds was determined with LC-MS/MS instrument. As a result, 12 phenolic compounds were quantitatively detect, among the 27 phenolic compounds analyzed. These; Quinic acid (618±29.66 mg g -1 ), Malic acid (809.88±42.92 mgg -1 ), tr-Aconitic acid (4.49±0.22 mgg -1 ), Gallic acid (2.73±0.14 mgg -1 ), Protocatechuic acid (24.11±1.73 mgg -1 ), Tannic acid (33.01±1.68 mg g -l ), tr-Coffeic acid (11.06±0.58 mg g -l ), Vanilin (9.18±0.45 mgg -1 ), p-Coumaric acid (61.16±3.12 mgg -1 ), Kaempferol (43.64±2.27 mgg -1 ), Luteolin (5.35±0.37 mgg -1 ) and Apigenin (8.27±0.44 mgg -1 ).Öğe The antioxidant capacity, total phenolic content and phenolic compounds of Spergularia rubra (L.) J. Presl & C. Presl in naturally distributed five different saline areas in Siirt province(IEEE, 2018) Tegin, Ibrahim; Canpolat, Gurbet; Fidan, MehmetSpergularia rubra has a very wide spread in the world. Infusion of S. rubra is used for diuretic purposes. Although widely used, the antioxidant activity of this species has not been evaluated and its chemical composition is not known [1]. In this study, antioxidant activity (DPPH %), total phenolic content (Folin Ciocalteau method) and phenolic compounds profile (LC-MS/MS) of methanolic extract of Spergularia rubra plant that collected from five different localities in Siirt were analyzed. According to the results of the analysis, the total phenolic content range from 4.33 to 13.16 mg gallic acid/mL extract. The highest antioxidant activity value was found as 77.70 %, on the other hand, the lowest was found as 57.32 %. As a result of the LC- MS/MS analysis for Cöl (Bayraktepe), location, 18 phenolic compounds were quantitatively and qualitatively determined, among the 27 phenolic compounds. The all values obtained (μg analyte/kg extract) were as follows; Quinic acid (4788.53±229.85), Malic acid (9618.56±509.78), tr-Aconitic acid (96.76±4.74), Gallic acid (141.54±7.22), Protocatechuic acid (56.17±2.86), Tannic acid (6068.41±309.49), tr-Coffeic acid (2.55±0.13), Vanilin (30.02±1.47), p-Coumaric acid (48.64±2.48), Rutin (1.13±0.05), Hyperoside (4.47±0.2), 4-OH benzoic acid (9.26±0.4), Salicylic acid (13.3±0.6) Kaempferol (63.68±3.31), Naringenin (2.33±0.12), Luteolin (35.12±2.42) Apigenin (35.14±1.86) and Rhamnetin (29.28±1.78).Öğe Conductive Polymer-Based Nanocomposites as Powerful Sorbents: Design, Preparation and Extraction Applications(Taylor & Francis Inc, 2022) Canpolat, Gurbet; Dolak, Ibrahim; Kecili, Rustem; Hussain, Chaudhery Ghazanfar; Amiri, Amirhassan; Hussain, Chaudhery MustansarConductive polymers as composite materials have been attracted tremendous attention due to their versatile and excellent features such as tunable conductivity, facile synthesis and fabrication, high chemical and thermal stability etc. These characteristics make them versatile and let them being used in numerous fields including microelectronics, optics and biosensors. Throughout the mentioned fields, conductive polymers particularly perform as effective sorbents. Although tremendous efforts have been put into this topic, to the best of our knowledge, a comprehensive up-to-date review on the applications of conductive polymers as efficient sorbents has not been reported. The main objective of this paper is to make a significant contribution to the recent literature toward the synthesis and extraction applications of conductive polymers as efficient sorbents.Öğe Designing copper-doped zinc oxide nanoparticle by tobacco stem extract-mediated green synthesis for solar cell efficiency and photocatalytic degradation of methylene blue(Taylor & Francis Inc, 2024) Ekinci, Arzu; Sahin, Omer; Kutluay, Sinan; Horoz, Sabit; Canpolat, Gurbet; Cokyasa, Mine; Baytar, OrhanThis study presents the green synthesis of copper-doped zinc oxide (Cu-doped ZnO) nanoparticles using tobacco stem (TS) extract. The environmentally friendly synthesis method ensures distinct features, high efficiency, and applicability in various fields, particularly in solar cell technology and photocatalytic applications. ZnO nanostructures are investigated due to their unique properties, cost-effectiveness, and broad range of applications. The nanoparticles are synthesized with varying Cu concentrations, and their structural, morphological, and compositional characteristics are thoroughly analyzed. The Cu-doped ZnO nanoparticles exhibit improved properties, such as increased surface area and reduced particle size, attributed to the incorporation of Cu dopants. The green synthesis approach using TS extract serves as a stabilizing agent and avoids the toxicity associated with chemical methods. Characterization techniques including SEM, TEM, EDX, FTIR, and XRD confirm the successful synthesis of the nanoparticles. Photocatalytic degradation studies reveal that the 5% Cu-doped ZnO exhibits the highest photocatalytic activity against methylene blue, attributed to synergistic effects between Cu and ZnO, including oxygen vacancy and electron-hole pair recombination rate suppression. The photocatalytic mechanism involves the generation of superoxide and hydroxyl radicals, leading to methylene blue degradation. Furthermore, the Cu-doped ZnO nanoparticles demonstrate promising photovoltaic performance, with the optimal efficiency observed at a 5% Cu concentration. The study suggests that Cu-doped ZnO has the potential to enhance solar cell efficiency and could serve as an alternative material in solar cell applications. Future research should focus on refining Cu-doped ZnO for further improvements in solar energy conversion efficiency The successful synthesis of Cu-doped ZnO nanoparticles from tobacco stem extract suggests an environmentally friendly approach. The photocatalytic degradation studies demonstrate the superior activity of 5% Cu-doped ZnO against methylene blue, attributed to synergistic effects between Cu and ZnO. Cu-doped ZnO has the potential to significantly enhance solar cell efficiency. The demonstrated photocatalytic and photovoltaic activities of Cu-doped ZnO open avenues for further research in optimizing their performance for practical applications in solar energy technologies and sustainable energy production.Öğe Determination of elemental contents of Lepidium Perfoliatum L. plant growing in saline area with ICP-OES(Academic Perspective Procedia, 2018) Teğin, İbrahim; Canpolat, GurbetLepidium Perfoliatum L. is one of the largest species of Brassicaceae family and has about 175 species [1]. It spreads worldwide, primarily in temperate and subtropical regions. In Arctic climates, the breed is rarely represented; and in tropical regions it grows in mountainous areas [2].There are 17 taxa in the genus Lepidium of Turkey [3-4]. In this study, the levels of 17 elements (As, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, Pb, Sb, Ti, V and Zn) were determined in Lepidium L. plants that grown in the Üzümlük and Çaykaya salty areas of Siirt, Turkey by inductively coupled plasma-optical emission spectrometry (ICP-OES). It was determined that the highest values were in Na, K, Mg and Fe for both areas. The all values obtained (mg L-1) were as follows; As (0.03), Co (0.01), Cr (0.06), Cu (0.34), Fe (10.97), K (91.21), Li (0.06), Mg (67.06), Mn (0.90), Mo (0.02), Na (189.20), Ni (0.12), Pb (0.31), Sb (0.02), Ti (0.13), V (0.02), Zn (0.27) for Üzümlük and Co (0.03), Cr (0.25), Cu (0.40), Fe (61.75), K (456.00), Li (0.18), Mg (138.80), Mn (4.22), Mo (0.01), Na (122.90), Ni (0.38), Pb (0.08), Sb (0.02), Ti (0.27), V (0.13) and Zn (0.63) for Çaykaya.Öğe Development of molecular imprinting-based smart cryogels for selective recognition and separation of serum cytochrome-c as a biochemical indicator(Elsevier Sci Ltd, 2021) Canpolat, Gurbet; Dolak, Ibrahim; Onat, Ruken; Kecili, Rustem; Baysal, Zubeyde; Ziyadanogullari, Berrin; Ersoz, ArzuThe recognition and detection of proteins has an important role in the fields of biochemistry and medicine in term of diagnosis and prognosis. In this study, molecularly imprinted cryogel (MIP) was synthesized for the selective recognition of Cytochrome-c (Cyt-c) by cryopolymerization techniques with the lanthanide-chelate approach using (MAAP)2-Ce(III) as complex functional monomer and Cyt-c as a template protein. The incorporation of template with monomer was characterized by near- infrared (Near-IR) spectroscopy. The binding capacity was optimized in accordance with numerous experimental parameters including pH, initial Cyt-c concentration, flow rate, temperature and ionic strength. As a result, the maximum binding capacity reached to 98.33 mg g-1 in a buffer system with a pH of 6.0. Beside of the excellent reusability performance of prepared MIP, it was successfully applied to separation of template Cyt-c in the existence of hemoglobin (Hb) and myoglobin (Mb) chosen as interfering proteins. The relative selectivity constants of the Cyt-imprinted polymer were determined as 13.84 for the Cyt/Mb pair and 16.05 for the Cyt/Hb pair. Consequently, a high selectivity, simple and cheap strategy for efficient separation of proteins was provided with this novel MIP cryogel.Öğe Dicle Nehri'nin Belirli İstasyonlarında Unio tigridis Türü ve Su Örneklerinde Ağır Metal Birikiminin İncelenmesi(2019) Abay, Çiğdem Kaymak; Canpolat, GurbetBu çalışmada, Dicle Nehri üzerinde Diyarbakır (I) ve Hasankeyf (II) olarak belirlenen iki istasyondan örneklenenUnio tigridis türünün iç organ kitlesi ve suda (Cu, Cd, Ni, Pb, Zn, Co ) ağır metallerin birikimleri incelenmiştir.İstasyon sularının bazı fiziko-kimyasal parametreleri çalışma alanında, örneklerdeki ağır metal birikim oranı iseICP-OES ile ölçülmüştür. Su örneklerinde iki istasyonda da en yüksek değerler Zn'da, en düşük değerler Cu'datespit edilmiştir. Ni sadece I. istasyonda ölçülebilmişken Co, Cd ve Pb’da değerler ICP-OES'in analiz limitininaltında kalmıştır. Unio tigridis türünde istasyonlar arasındaki farklılık parametrik bir test olan t-test ile yapılmıştır.Unio tigridis’te istasyonlar arasındaki ağır metal birikimine bakıldığında Ni, Cu ve Pb da en yüksek değerler I.istasyonda; Co ve Zn da ise II. istasyon da ölçülmüştür. Cu, Pb ve Zn yoğunluklarında istatistik açısından farkönemli bulunmuştur (p<0.05).Öğe Eruh bölgesinden toplanan Allıum ampeloprasum L. bitkisinin element içeriklerinin ICP-MS ile belirlenmesi(İKSAD YAYINEVİ, 2018) Teğin, İbrahim; Canpolat, Gurbet; Fidan, MehmetAllium L. (Soğan) cinsi yeryüzünde yaklaşık 850 taksona sahip olup Türkiye'de 179 (197 takson) Allium türü mevcuttur (Keusgen ve ark., 2011; Güner ve ark., 2012). Birçok etnobotanik çalışmada Allium türlerinin farklı amaçlarla kullanıldığı rapor edilmiştir. Genel olarak, Allium'un tüm bitki kısımları insanlar tarafından tüketilebilmekte (tohumlar hariç) (Fritsch ve Friesen, 2002). Bu çalışma kapsamında, Eruh’un yüksek kesimlerinden toplanan Allium ampeloprasum L. bitkisinin element analizi ICP-MS ile yapılmıştır. Bu bitkide bulunan Li, B, Na, Mg, Al, P, K, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Se, Sr, Mo, Ag, Cd, Ba, La, Ce, Pb' nun değerleri sırasıyla 0,76±0,09, 17,38±6,16, 59,35±20,35, 1473,81±0,24, 636,67±0,09, 1253,76±0,14, 1554,25±3,24, 4353,59±0,02, 4,68±1,04, 38,58±9,01, 994,06±0,15, 0,90±0,02, 5,03±0,47, 4,22±1,02, 20,53±1,57, 2,97±0,53, 0,48±0,07, 84,53±6,76, 11,92±1,33, 3,83±0,12, 0,40±0,03, 1,51±0,12, 5,44±1,27, 0,50±0,13, 0,94±0,13 ve 0,79±0,16 mg/L olarak bulunurken; In, Sn, Sb, Pt, Tl ve Bi değerleri 80,29±0,02, 63,74±0,01, 194,93±0,04, 13,23±0,00, 11,97±0,00 ve 7,32±0,00 μg/L olarak bulunmuştur.Öğe Functionalized magnetic nanoparticles as powerful sorbents and stationary phases for the extraction and chromatographic applications(Elsevier Sci Ltd, 2021) Kecili, Rustem; Ghorbani-Bidkorbeh, Fatemeh; Dolak, Ibrahim; Canpolat, Gurbet; Karabork, Muharrem; Hussain, Chaudhery MustansarMaterials in nanodimensions such as magnetic nanoparticles exibit fascinating physico-chemical features (i.e. high surface area, very small size, superparamagnetism, active surface which can be successfully functionalized, low toxicity, etc). Because of these superior features, functionalized magnetic nanoparticles received increasing attention for tremendous amount of studies over the last years and a number of successful applications were published. This paper aims to present and highlight the latest progresses in the development of novel functionalized magnetic nanoparticles for the extraction and chromatographic applications. (C) 2021 Elsevier B.V. All rights reserved.Öğe Green ion–imprinted polymers(Elsevier, 2024) Canpolat, Gurbet; Dolak, İbrahimThe increasing concern over the environmental impact of traditional chemical synthesis methods has driven the need for environmentally friendly and sustainable alternatives. The principles of green chemistry have been established to guide the design of products and processes that minimize hazardous substances. Molecular imprinting, a rapidly growing field, offers a powerful synergy with green chemistry, allowing for the development of effective and sustainable chemical products for the selective recognizing of both ions and molecules. However, limited research has evaluated the compatibility of ion-imprinted polymers (IIPs) with the principles of green chemistry. This chapter provides a thorough overview of environmentally friendly IIP synthesis alternatives, including the use of greener reagents (dummy templates, functional monomers, porogenic solvent). The chapter highlights the use of computer-based simulation to predict functional monomers, identify binding methods, and assess specificity and demonstrates the use of green approaches in the development of selective IIPs for applications in sensing, separation, and purification. This review aims to offer researchers a fresh outlook on ion imprinting technology and inspire further innovative developments in the field of ion separation and purification. © 2024 Elsevier Inc. All rights are reserved including those for text and data mining AI training and similar technologies.Öğe Green nanomaterials in sample pre-treatment processes(Elsevier B.V., 2024) Canpolat, Gurbet; Dolak, İbrahim; Keçili, Rüstem; Hussain, Chaudhery MustansarExtraction is an important step in sample pre-treatment processes for the efficient removal of an analyte of interest from a complex matrix. Removal of harmful pollutants from contaminated food, environmental, and biological samples is a crucial demand. Green extraction techniques have been developed as greener and more sustainable alternatives to traditional sample preparation methods. These techniques aim to improve the selectivity and sensitivity of analytical methods and simultaneously reducing the harmful side effects of conventional extraction techniques. Green nanomaterials have advanced extraction technologies due to their unique physicochemical features and facile surface functionalization. This chapter presents the synthesis of green nanomaterials and their use in analytical applications, with a special focus on their roles as sorbent materials. © 2024Öğe İmmobilize Coriolus versicolor kullanılarak Pb (II) iyonlarının biyosorpsiyonu ve önderiştirilmesi(İKSAD ULUSLARARASI YAYINEVİ, 2018) Okumuş, Veysi; Akcan, Nurullah; Canpolat, GurbetAğır metaller gıda sektörünün başlıca kirleticileri arasında yer alır ve özellikle bizim gibi gelişmekte olan ülkelerde en önemli sorunlardan biri olarak düşünülebilir. Gıdalardaki ağır metallerin aşırı içeriği, özellikle kardiyovasküler, böbrek, sinir ve diğer hastalıklarla birlikte bir dizi hastalığın etiyolojisi ile ilişkilidir. Kurşun, ciddi bir kümülatif vücut zehiridir ve vücut sistemimize hava, su ve yiyecek yoluyla girer. Bazı ülkelerde insanlar, içme suyunun kurşun borulardan kaynaklanan kirlenmeleri nedeniyle önemli ölçüde bu metale maruz kalabilir. Kurşun kirliliğinin çoğu kümülatif etki gösterir ve insanlar kurşun ile uzun süre teması sonucunda, küçük konsantrasyonlarda bile sağlığa zararlı olabilir. Gıda ve su örneklerinde kurşun gibi metal iyonlarının aletli analizle doğrudan belirlenmesi, düşük miktarda olmaları nedeniyle çoğu zaman zordur. Bu nedenle, kantitatif olarak belirlenmeden önce genellikle bir ayırma ve önderiştirme tekniği gereklidir. Bu çalışmada Katı faz ekstraksiyonu (SPE) yöntemi ile Pb iyonlarının önderiştirilmesi için alternatif bir yöntem geliştirilmiştir., Pb'nin indüktif eşleşmiş plazma optik emisyon spektrometrisi (ICP-OES) ile belirlenmesinden önce önderiştirilmesi için immobilize Coriolus versicolor fungal biyosorbent olarak kullanıldı. Geliştirilen SPE yöntemindeki bazı parametreler (pH, akış hızı, biyosorbent miktarı, kolonun tekrar kullanılabilirliği) optimize edildi. Yöntemin doğruluğu sertifikalı referans çay numunelerinin analizi ile doğrulandı. Geliştirilen kolonun 30 kullanımdan sonra bile oldukça stabil olduğu belirlendi.Öğe Metal chelate based site recognition of ceruloplasmin using molecularly imprinted polymer/cryogel system(Taylor & Francis Group, 2020) Dolak, Ibrahim; Canpolat, Gurbet; Ersöz, Arzu; Say, RıdvanIn the present study, N-Acetylneuraminic acid (NANA)-imprinted poly(2-hydroxyethyl methacrylate- N-methacryloyl-(L)-histidin-Cu(II)) p(HEMA-(MAH)2-Cu(II)) has been synthesized by radical polymerization for site recognition of ceruloplasmin. Prepared imprinted cryogel has been characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). When the binding capacity of NANA-imprinted cryogel (MIP) was compared with nonimprinted cryogel (NIP), it was found that MIP has higher adsorption capacity. The maximum amount of binding NANA has found as 83.20 mgg−1 at pH 7.0 with flow rate of 1 mLmin−1 at 25°C. Selectivity experiments for MIP and NIP cryogel have been carried out via the NANA/D-mannose and ceruloplasmin/immunoglobulin G (IgG) pair separately and the relative selectivity coefficient (k’) has found as 77.46 and 17.97, respectively. Applicable of the MIP in human serum has been studied and resulted successfully.Öğe Metal-Free Coal Catalyst for Hydrogen Production: Synthesis and Performance Assessment(2023) Canpolat, Gurbet; Kaya, MustafaIn this study, a coal-based catalyst produced by protonating phosphoric acid was used as a metal-free catalyst for hydrogen production from sodium borohydride (NaBH4) methanolysis. Experiments were conducted with various acid concentrations, impregnation times, and carbonization temperatures and times in order to produce a metal-free coal catalyst with enhanced catalytic activity. The catalyst impregnated with 3M H3PO4 for 12 h and subsequently carbonized at 600°C for 90 min exhibited the highest catalytic activity. The hydrogen production at 60 °C methanolysis with 0.25 g of NaBH4 catalyzed by a metal-free coal catalyst was found to be 11,854 mL min?1g.cat?1. Additionally, the activation energy of the catalyst was determined to be 22.5 kJ mol-1.Öğe Microwave-assisted sumac based biocatalyst synthesis for effective hydrogen production(Springer, 2024) Canpolat, GurbetHydrogen (H-2), a renewable energy source with a high energy density and a reputation for being environmentally benign, is being lauded for its potential in various future applications. In the present context, the catalytic methanolysis of sodium borohydride (NaBH4) is of considerable importance due to its provision of a pathway for the efficient production of hydrogen gas (H-2). The main aim of this research attempt was to assess the viability of utilizing refuse defatted sumac seeds as an unusual precursor in microwave-assisted K2CO3 activation to produce a biocatalyst. The primary objective that motivated the synthesis of the biocatalyst was to facilitate the generation of hydrogen via the catalytic methanolysis of NaBH4. With the aim of developing a biocatalyst characterized by enhanced catalytic performance, we conducted an exhaustive investigation of a wide range of experimental parameters. The activation agent-to-sample ratio (IR), impregnation time, microwave power, and irradiation time were among these parameters. Significantly enhanced in catalytic activity, the biocatalyst produced under particular conditions achieved a peak hydrogen production efficiency of 10,941 mL min(- 1) g.cat(- 1). In particular, it was determined that the ideal conditions were as follows: 0.5 IR, 24 h of impregnation, 500 W of microwave power, and 10 min of irradiation. This novel strategy not only demonstrates the impressive potential of eco-friendly biocatalysts, but also positions them as a viable alternative material for the sustainable production of hydrogen via NaBH4 methanolysis. Three significant parameters contribute to the value and renewability of this study. The first is that waste is used as the primary material; the second is that the activator is less hazardous than other activators; and the third is that microwave activation is a green chemistry technique.Öğe Molecularly imprinted polymer-based microspheres for selective extraction of hemoglobin from blood serum(Elsevier Sci Ltd, 2023) Canpolat, GurbetIn this study, a highly selective molecularly imprinted microsphere (Hb-MAFol-Nd(III)-MIP) was prepared with ion-chelate approach for the extraction of hemoglobin (Hb) from blood serum. The Hb-imprinted microspheres were synthesized by suspension polymerization. For this purpose, Hb, N-methacryloylamino folic acid-Nd(III) (MAFol-Nd(III)), ethtylene glycol dimethacrylate (EDMA) and azobisisobutyronitrile (AIBN) were used as the template, complex functional monomer, cross-linker and initiator, respectively. Imprinted and NIP microspheres were characterized by Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). The binding efficiency of the template on both MIP and NIP was optimized with respect to various parameters including pH, contact time, and hemoglobin concentration. As a result, the maximum binding capacity was observed to be as 361 mg g(-1) at the pH value 7.0. For selectivity study, myoglobin (Mb), cytochrome c (Cyt) and lysozyme (Lys) were chosen as competitive proteins. The prepared imprinted microspheres exhibited high selectivity toward template based on the selectivity coefficients (k). The extraction of Hb from spiked blood serum sample was performed with excellent success. From the correlation coefficients, isotherm study indicated that the adsorption of Hb by MIP fit a Langmuir model.Öğe Sample Preparation with Conductive Polymers(American Chemical Society, 2022) Canpolat, Gurbet; Dolak, Ibrahim; Hussain, Chaudhery Ghazanfar; Keçili, Rüstem; Hussain, Chaudhery MustansarThis chapter presents and highlights the latest progress in the synthesis andapplications of conductive polymers and their nanocomposites as powerfulsorbents in different sample preparation techniques including solid-phaseextraction, solid-phase microextraction, dispersive solid-phase extraction, andmagnetic solid-phase extraction. The review covers the extraction of heavy metalsand organic analytes from various food, environmental, and biological samplessuch as water, urine, and blood. In addition, electrochemical electrodes based onCPs and their composites are also summarized. © 2022 American Chemical Society. All rights reserved.Öğe Selective separation of cobalt using a new Co(II)-imprinted polymer/cryogel system(2018) Canpolat, GurbetThe aim of the present study was to investigate selective separation and preconcentration of Co(II) ions in aqueous solutions by ion imprinted technique. For this purpose, Co(II)-methacryloylhistidine((MAH)2-Co(II)) complex monomer has been synthesized and polymerized with presence of 2-hydroxyethyl methacrylate (HEMA) followed by flushed out of bonded Co(II) ions from polymer with 5.0 mol*L-1 HNO3. Thus, specific cavities for re-binding of target ions (Co(II)) have been created onto p-HEMA-MAH cryogel column. Prepared metal complex and Co-imprinted polymer/cryogel system have been characterized by FTIR and SEM. The effect of various parameters such as pH, initial concentration, flow rate, ionic strength have been investigated to determine optimal adsorption conditions. The maximum amount of adsorbed Co(II) has found as 106.0 mg*g-1 at pH 8.0 with flow rate of 1 mL*min-1. For selectivity experiments, binding studies were carried out presence of Cu(II) and Ni(II). The k (selectivity coefficient) values have found as 8.9 and 3.8 for Co(II)/Cu(II) and Co(II)/Ni(II) binary mixture, respectively. The reuse of column also has been investigated and there was no significant decrease at adsorption capacity even in consecutive eleven experiments.Öğe Siirt Bayraktepe (Çöl) tuzlu alanda dağılım gösteren Crypsis schoenoides (L.) Lam'ın antioksidan kapasitesi ve toplam fenolik içeriğinin belirlenmesi(İKSAD YAYINEVİ, 2018) Teğin, İbrahim; Canpolat, Gurbet; Fidan, MehmetCrypsis schoenoides (L.) Lam. Bozkır bölgesinden meşe ormanlarına kadar sporadik olarak bulunabilen tek yıllık bir halofid türdür. Bataklık alanlar, yüzülebilir alanlar, tuzlu topraklar, geçici olarak su altında kalan alanlar, humus içeriği çok düşük alanlarda yetişir (Simeanu, 2003). Eragrostis aegyptiaca (Willd.) Delile, Fimbristylisbis umbellata (Forsk.) Bub. ve Crypsis schoenoides gibi çimler, su baskını sonrası Nasser Gölü'nün kıyısındaki hayvancılık için ana otlatma kaynağıdır (Badri ve Hamed, 2000). Crypsis Poaceae familyasının bir cinsi olup Türkiye’de 7 taksonu bulunmaktadır (Güner ve ark., 2012). Bu çalışmada, Crypsis schoenoides'in metanol ile hazırlanan ekstraksiyonlarının analizi sonucunda; % DPPH 60,50, toplam fenolik içerik 12,16 mg Gallik/mLekstre olarak tespit edilmiştir. Ayrıca fenolik bileşiklerin kimyasal bileşimi LC-MS / MS cihazı ile belirlenmiştir Analiz edilen 27 fenolik bileşik arasında 14 fenolik bileşik kalitatif ve kantitatif olarak belirlenmiştir. Bunlar; Quinic asit (21156,22 ± 1015,50 μg g-1), Malik asit (13266,00 ± 703,10 μg g-1), tr-Aconitic asit (11,10 ± 0,54 μg g-1), Gallik asit (10,95 ± 0,56 μg g-1), Protocatekuik asit (529,29 ± 26,99 μg g-1), Tannik asit (330,61 ± 16,86 μg g-1), tr-Coffeik asit (1,85 ± 0,10 μg g-1), Vanilin (2,19 ± 0,11 μg g-1), p-Coumarikacid (112,85 ± 5,76 μg g-1), Hiperosid (241,00 ± 11,81 μg g-1), Quercetin (193,42 ± 13,73 μg g-1), Kaempferol (1,72 ± 0,09 μg g-1), Hesperetin (10,00 ± 0,53 μg g-1) ve Rhamnetin (68,26 ± 4,16 μg g-1) olarak tespit edilmiştir.
