Production and Optimization Parameters of Amylases from Bacillus subtilis RSKK96 Under Solid State Fermentation

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dc.authoridakcan, Nurullah/0000-0003-3960-9553
dc.contributor.authorAkcan, N.
dc.contributor.authorSerin, B.
dc.contributor.authorUyar, F.
dc.date.accessioned2024-12-24T19:33:12Z
dc.date.available2024-12-24T19:33:12Z
dc.date.issued2012
dc.departmentSiirt Üniversitesi
dc.description.abstractThe production of extracellular a-amylase by producing Bacillus subtilis RSKK96 was studied under solid state fermentation (SSF). Various agroresidues as substrate were studied for enzyme production. The highest enzyme yield was expressed with cotton stalk substrate (1016.3 +/- 32.6 U g(-1) . 10(-3)). Production parameters were optimized as incubation time 72 hours, incubation temperature 37 degrees C, agitation speed 150 rpm, inoculum size 35 %, initial moisture content 30 %, initial pH 7.0. Supplementation of the fermentation medium with carbon and metal salt sources decreased the enzyme production. Among different nitrogen sources supplemented, ammonium nitrate (1 %) showed maximum enzyme production (1483.1 +/- 32.5 U g(-1) . 10(-3)). Enzyme activity was observed as 73 % at 100 degrees C.
dc.description.sponsorshipscientific Research and Project Coordinator in University (DUAPK) of Dicle [04-FF-47]
dc.description.sponsorshipThis research was supported by the scientific Research and Project Coordinator in University (DUAPK, 04-FF-47) of Dicle.
dc.identifier.endpage239
dc.identifier.issn0352-9568
dc.identifier.issn1846-5153
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84868125215
dc.identifier.scopusqualityQ3
dc.identifier.startpage233
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8023
dc.identifier.volume26
dc.identifier.wosWOS:000310403500010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherCroatian Soc Chemical Engineering Technology
dc.relation.ispartofChemical and Biochemical Engineering Quarterly
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241222
dc.subjectalpha-Amylase
dc.subjectBacillus subtilis RSKK96
dc.subjectsolid substrate fermentation
dc.subjectcotton stalk
dc.titleProduction and Optimization Parameters of Amylases from Bacillus subtilis RSKK96 Under Solid State Fermentation
dc.typeArticle

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