AMELIORATIVE EFFECT OF ASTAXANTHIN ON ISCHEMIA-REPERFUSION INJURY OF SKELETAL MUSCLES

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dc.authoridbengu, aydin sukru/0000-0002-7635-4855
dc.contributor.authorUyar, A.
dc.contributor.authorAkkoyun, H. T.
dc.contributor.authorBengu, A. S.
dc.contributor.authorAkkoyun, M. B.
dc.contributor.authorKeles, O. f.
dc.contributor.authorAtcali, T.
dc.contributor.authorMelek, S.
dc.date.accessioned2024-12-24T19:33:51Z
dc.date.available2024-12-24T19:33:51Z
dc.date.issued2022
dc.departmentSiirt Üniversitesi
dc.description.abstractThis experimental study aimed to investigate the ameliorative effect of astaxanthin (AST) on the prevention of skeletal muscle injury resulting from lower extremity ischemia/reperfusion (I/R). Twenty-eight (250-300g) male Wistar albino rats were divided into 4 groups as Control, I/R, I/R+AST and AST. In the control group, only anesthesia was induced for 2 h without I/R. In the I/R group, 2 h of reperfusion was facilitated following ischemia under anesthesia. For the I/R+AST group, 7 days prior to ischemia, 125 mg/kg AST was given through a gavage, and 2 h of ischemia and 2 h of reperfusion were facilitated under anesthesia. At the end of the study, blood and gastrocnemius muscle tissue samples were taken for biochemical, histopathological and immunohistochemical examinations. Compared to the control group, there were increased Malondialdehyde (MDA) levels and decreased Superoxide dismutase (SOD) and Catalase (CAT) enzyme activities in the I/R group (p??0.001). Degeneration, necrosis, inflammation, loss of striation, interfibrillar and interfascicular edema were seen in the histopathological examination of the skeletal muscles in the I/R group. These histopathological findings were minimal in the I/R+AST group. In the immunohistochemical examination of muscle tissue with the GPx1 primary antibody, a mild degree of GPx1 reactivity was observed in the I/R group, and a moderate degree of GPx1 reactivity was seen in the I/R+AST group. As a result, the strong ameliorative effect of AST on ischemia-reperfusion injury and its complications on skeletal muscles was demonstrated by biochemical, histopathological and immunohistochemical examinations.
dc.identifier.doi10.36899/JAPS.2022.4.0494
dc.identifier.endpage933
dc.identifier.issn1018-7081
dc.identifier.issn2309-8694
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85138742944
dc.identifier.scopusqualityQ3
dc.identifier.startpage924
dc.identifier.urihttps://doi.org/10.36899/JAPS.2022.4.0494
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8322
dc.identifier.volume32
dc.identifier.wosWOS:000835073500004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPakistan Agricultural Scientists Forum
dc.relation.ispartofJournal of Animal and Plant Sciences-Japs
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241222
dc.subjectIschemia-reperfusion
dc.subjectMuscle
dc.subjectAstaxanthin
dc.subjectMDA
dc.subjectSOD
dc.subjectCAT
dc.subjectHistopathology
dc.titleAMELIORATIVE EFFECT OF ASTAXANTHIN ON ISCHEMIA-REPERFUSION INJURY OF SKELETAL MUSCLES
dc.typeArticle

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