In vitro effect of platelet-rich plasma (PRP) on morpho-functional characteristics, oxidative stress, apoptosis and CatSper-1 ion channel in short-term stored (5 °C) ram semen

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dc.contributor.authorGaffari Türk
dc.contributor.authorİbrahim Halil Güngör
dc.contributor.authorGözde Arkalı
dc.contributor.authorAslıhan Çakır Cihangiroğlu
dc.contributor.authorNida Badıllı
dc.contributor.authorTutku Can Acisu
dc.contributor.authorSerap Dayan Cinkara
dc.contributor.authorŞuheda Yüksel
dc.contributor.authorŞeyma Özer Kaya
dc.contributor.authorMustafa Sönmez
dc.contributor.authorSeyfettin Gür
dc.contributor.authorAbdurrauf Yüce
dc.date.accessioned2025-02-18T08:26:19Z
dc.date.available2025-02-18T08:26:19Z
dc.date.issued2025-03
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü
dc.description.abstractThe objective of this study was to examine the impact of platelet-rich plasma (PRP) on the morpho-functional characteristics, oxidative stress, apoptosis and CatSper-1 ion channel in short-term stored ram semen at 5 °C. The study was conducted in six independent replicates on pooled semen samples collected by artificial vagina from six sexually active Akkaraman rams. The pooled semen samples were diluted to a concentration of 400 million spermatozoa per milliliter with a tris-based diluent including 6 PRP concentration (1, 2, 4, 8, 10, 20 %) and no PRP (control) at 38 °C. Following the reduction of the temperature to 5 °C, diluted semen samples were stored for a period of 96 hours. Motility (total, progressive, rapid, medium, low), kinematic (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), morphological (plasma membrane integrity, abnormality), flow-cytometric (mitochondrial activity, acrosomal damage, viability, apoptosis), oxidative stress (MDA, GSH, GSH-Px, CAT), and CatSper-1 analyses were performed at both hours zero (initial measurement) and 96. In all groups, a time-dependent deterioration was observed in nearly all parameters at 96 h in comparison to the 0 h. Compared to the control, live spermatozoon rates (8 % and 10 %), GSH-Px (4 %, 8 % and 10 %) and CAT (4 % and 8 %) activities, and CatSper-1 levels at 96 h were higher, while the apoptotic spermatozoon rates (8 % and 10 %) and MDA levels (4 %, 8 % and 10 %) were lower in semen samples containing PRP. However, in semen samples containing 20 % PRP, the apoptotic spermatozoon rates and MDA levels at 96 h were higher, while GSH-Px activity and CatSper-1 levels were lower than in the control. As a result, it was concluded that the addition of 4–10 % PRP to the diluent containing tris+egg yolk has an effective role in preventing oxidative, apoptotic and ion channel (CatSper-1) damages in ram spermatozoa stored for a short time at 5 °C, while the concentration of 20 % is harmful.
dc.identifier.citationTürk, G., Güngör, İ. H., Arkali, G., Cihangiroğlu, A. Ç., Badilli, N., Acisu, T. C., ... & Yüce, A. (2025). In vitro effect of platelet-rich plasma (PRP) on morpho-functional characteristics, oxidative stress, apoptosis and CatSper-1 ion channel. in short-term stored (5° C) ram semen. Small Ruminant Research, 107449.
dc.identifier.doi10.1016/j.smallrumres.2025.107449
dc.identifier.issn0921-4488
dc.identifier.scopus2-s2.0-85217026888
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.smallrumres.2025.107449
dc.identifier.urihttps://hdl.handle.net/20.500.12604/8526
dc.identifier.volume244
dc.indekslendigikaynakScopus
dc.institutionauthorCihangiroğlu, Aslıhan Çakır
dc.institutionauthorid0000-0003-3365-8960
dc.language.isoen
dc.publisherElsevier BV
dc.relation.ispartofSmall Ruminant Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApoptosis
dc.subjectCatSper-1
dc.subjectOxidative stress
dc.subjectPlatelet-rich plasma
dc.subjectRam
dc.subjectSemen
dc.titleIn vitro effect of platelet-rich plasma (PRP) on morpho-functional characteristics, oxidative stress, apoptosis and CatSper-1 ion channel in short-term stored (5 °C) ram semen
dc.typejournal-article
oaire.citation.volume244

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